Molecular mapping of<i>YrTZ2</i>, a stripe rust resistance gene in wild emmer accession TZ-2 and its comparative analyses with<i>Aegilops tauschii</i>

Wang, Zhenzhong; Xie, Jingzhong; Guo, Li; Zhang, Deyun; Li, Genqiao; Fang, Tilin; Chen, Yongxing; Li, Jun; Wu, Qiuhong; Lü, Ping; Wang, Yong; Li, Miaomiao; Wu, Haixin; Zhang, Yan; Yang, Wuyun; Luo, Ming‐Cheng; Fahima, Tzion; Li, Yong

doi:10.1101/131003

Cited by 5 publications

(4 citation statements)

References 55 publications

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“…5, Table S3). Thus, other genes reported to be located on 1BS chromosome, especially these originating from WEW, such as YrSM139-1B (Zhang et al , 2016) and YrTz2 (Wang et al , 2018), should be tested for allelism/identity to Wtk1 .…”

Section: Discussionmentioning

confidence: 99%

Three previously characterized resistances to yellow rust are encoded by a single locusWtk1

Klymiuk

Fatiukha

Raats

et al. 2020

Preprint

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The wild emmer wheat (Triticum turgidum ssp. dicoccoides; WEW) yellow (stripe) rust resistance genes Yr15, YrG303 and YrH52 were discovered in natural populations from different geographic locations. They all localize to chromosome 1B but were thought to be non-allelic based on differences in resistance response. We recently cloned Yr15 as a Wheat Tandem Kinase 1 (WTK1) and showed here that these three resistance loci co-segregate in fine-mapping populations and share identical full-length genomic sequence of functional Wtk1. Independent EMS mutagenized susceptible yrG303 and yrH52 lines carried single nucleotide mutations in Wtk1 that disrupted function. A comparison of the mutations for yr15, yrG303 and yrH52 mutants showed that while key conserved residues were intact, other conserved regions in critical kinase subdomains were frequently affected. Thus, we concluded that Yr15-, YrG303- and YrH52-mediated resistances to yellow rust are encoded by a single locus Wtk1. Introgression of Wtk1 into multiple genetic backgrounds resulted in variable phenotypic responses, confirming that Wtk1-mediated resistance is part of a complex immune response network. WEW natural populations subjected to natural selection and adaptation have potential to serve as a good source for evolutionary studies of different traits and multifaceted gene networks.HighlightWe demonstrate that Yr15, YrG303 and YrH52 resistances are encoded by the Wtk1 locus, but express variable resistance responses to yellow rust in a genetic background dependent manner.

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Section: Discussionmentioning

confidence: 99%

Three previously characterized resistances to yellow rust are encoded by a single locusWtk1

Klymiuk

Fatiukha

Raats

et al. 2020

Preprint

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“…Wild emmer wheat accession IW2 was also resistant to stripe rust (Wang et al ., 2018). Sequence amplification revealed that IW2 also contains the broad‐spectrum stripe rust resistance gene Yr15 .…”

Section: Discussionmentioning

confidence: 99%

A CNL protein in wild emmer wheat confers powdery mildew resistance

Dong

et al. 2020

New Phytologist

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Powdery mildew, a fungal disease caused by Blumeria graminis f. sp. tritici (Bgt), has a serious impact on wheat production. Loss of resistance in cultivars prompts a continuing search for new sources of resistance. Wild emmer wheat (Triticum turgidum ssp. dicoccoides, WEW), the progenitor of both modern tetraploid and hexaploid wheats, harbors many powdery mildew resistance genes. We report here the positional cloning and functional characterization of Pm41, a powdery mildew resistance gene derived from WEW, which encodes a coiled-coil, nucleotide-binding site and leucine-rich repeat protein (CNL). Mutagenesis and stable genetic transformation confirmed the function of Pm41 against Bgt infection in wheat. We demonstrated that Pm41 was present at a very low frequency (1.81%) only in southern WEW populations. It was absent in other WEW populations, domesticated emmer, durum, and common wheat, suggesting that the ancestral Pm41 was restricted to its place of origin and was not incorporated into domesticated wheat. Our findings emphasize the importance of conservation and exploitation of the primary WEW gene pool, as a valuable resource for discovery of resistance genes for improvement of modern wheat cultivars.

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“…A. tauschii is the ancestor of the D genome in wheat and it was previously shown to encode resistance to powdery mildew (Majka et al, 2017), stem rust (Periyannan et al, 2013), leaf rust (Mohler et al, 2019), stripe rust (Wang et al, 2018), andSTB (McKendry &Henke, 1994). The latter study highlighted both qualitative and quantitative resistance among A. tauschii lines to STB disease, some of which exhibited isolate-specific resistance.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the susceptibility of modern, wild, ancestral, and mutational wheat lines to Septoria tritici blotch disease

et al. 2021

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Globally, bread wheat production is threatened by fungal diseases, including the devastating disease Septoria tritici blotch (STB). Given the global importance of STB, and the difficulty in identifying novel sources of resistance to this disease, we screened a variety of wheat genotypes, including wild, ancestral, and mutagenized lines, for their STB response. This delineated a panel of wild wheat relatives and Watkins collection lines with exceptional resistance to a range of Zymoseptoria tritici isolates, some of which are highly virulent on modern, elite wheat varieties. Additionally, we characterized the STB susceptibility of 500 lines of the wheat cultivar Cadenza TILLING population and developed backcross derivatives of two TILLING lines that show dominant partial resistance to STB. These backcross lines are partially resistant to multiple isolates of Z. tritici, and, with the wild and ancestral lines identified, provide a useful reservoir of STB‐resistant germplasm for use in wheat breeding programmes.

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Molecular mapping ofYrTZ2, a stripe rust resistance gene in wild emmer accession TZ-2 and its comparative analyses withAegilops tauschii

Cited by 5 publications

References 55 publications

Three previously characterized resistances to yellow rust are encoded by a single locusWtk1

Three previously characterized resistances to yellow rust are encoded by a single locusWtk1

A CNL protein in wild emmer wheat confers powdery mildew resistance

Evaluation of the susceptibility of modern, wild, ancestral, and mutational wheat lines to Septoria tritici blotch disease

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