Molecular Interaction of the src Gene Product with Cellular Adhesion Plaques

Effect of transformation by Rous sarcoma virus on the character and distribution of actin in Rat-1 fibroblasts: A biochemical and microscopical study

“…Using the vinculin antibody, Rat-I cells displayed strong staining in the adhesion plaques both at the cell periphery and under the cell body (Figure 4a) whereas the VIT cells showed concentrations of vinculin present only in small plaque-like structures which were largely confined to the cell periphery (Figure 4b). Because both the microfilament architecture and adhesion plaque formation appeared to be significantly perturbed by infection with the Rous sarcoma virus, it was of interest to examine the localisation of pp6Ov-src in these cells, since earlier studies had strongly suggested that this molecule was associated with adhesion plaques in transformed cells (Burr et al, 1980;Rohrschneider et al, 1983). Using a fluorescent labelled pp60src antibody applied to the VIT cells the fluorescence was seen to be mainly localised at the plasma membrane (Figure 5b) but it also seemed to be concentrated in adhesion plaque-like structures on the ventral surface of the cell (not shown here).…”

Altered cytoskeletal structures in transformed cells exhibiting obviously metastatic capabilities

“…Motivated by the cytosketetal changes induced by viral transformation [9,10] and by the indications that the oytoskeleton may be the target site for oncogene products [11], we have investigated the distribution of tubulin, F-actin, α−actinin and vinculin in human tumor DNA transformed cells exhibiting obviously metastatic capabilities. This transformed cell line LM-51 was derived from the lung metastasis of nude mice after subcutaneous inoculation of transformed cell clone which was obtained from the secondary transformants of NIH3T3 cells transfected with human highly metastatic tumor cell DNA [12].…”