Molecular Identification, Production and Optimization of Pectinase by using Stenotrophomonas maltophilia P9 Isolated from Algal Biomass of Himachal Pradesh, India

Sharma, Poonam; Sharma, Nupur

doi:10.20546/ijcmas.2018.701.082

Cited by 5 publications

(2 citation statements)

References 10 publications

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“…The results show that isolate B40, B32, B35 and B31 were the most effective isolates in pectinolytic activities with isolates B40 and B32 being the highest significant pectinase produces as compared with other isolates (Table 4). These results are in line with reports published by other authors [38][39][40].…”

Section: Screening Of Potential Pectinase Production By Ten Promisingsupporting

confidence: 94%

Retting and degumming of flax using biotechnology eco-friendly approach

Moawad¹,

El‐Rahim

Hashem

et al. 2019

Egypt. J. Chem.

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F LAX retting is a treatment process employed to dissolve or rot away much of the cellular tissues and pectins surrounding bast/fiber bundles thus facilitating separation of the fiber from the stem. The microbial retting is one of the most environmentally friendly process. The rich source of microbes contributing to flax retting is the retting water in commercial retting plants. The main objective of this work is to accelerate the flax retting using selected bacterial strains contributing to retting and degumming of flax in order to avoid chemicals causing environmental pollution. In this work, reuse of retting water for acceleration of flax retting was tested. The results show that, the reuse of retted water in new cycle of flax retting has accelerated the retting completion by 8.3-25% depending on the ratio of reused retted water in the retting liquor. Therefore, samples of flax straw retted water and soils from flax fields located in Nile Valley and Delta soils were collected for isolation of flax retting bacteria predominant in the retting effluents. Identification of the isolates was done using morphological, physiological and biochemical tests. Ten bacterial isolates were identified as Lactococcus cremoris, Chryseobacterium culicis, Serratia marcescens, Capnocytophage granulose, Micrococcus luteus, Enterobacter homraechei, Klebsiella oxytoca, Paenibacillus polymyxa, Proteus mirabilis and Bacillus humi. Three efficient strains in pectinase production necessary for flax retting were selected to test their role in enhancing the retting process. These strains are Bacillus humi, Chryseobacterium culicis and Micrococcus luteus which showed specific pectinase activity 37.17, 33.53 and 28 Umg 1 respectively. The effect of these strains on retting duration and fiber quality were studied. The combinations of the three strains were tested in the lab scale cylindrical experimental bioreactor and each of the combinations was compared with the classical retting process of the industrial units. The combined treatment using strains: Chryseobacterium culicis, Micrococcus luteus and Bacillus humi resulted in the reduction of the retting duration by 30%, whereas, the mixed inocula containing Chryseobacterium culicis and Micrococcusluteus reduced the retting time by 25%. The mixture of Micrococcus luteus and Bacillus humi reduced the retting duration by 20%. The determination of weight loss, tensile strength, whiteness and yellowness of the fibers after microbial retting was monitored. These results indicate that the obtained specific bacterial strains enhanced the retting process.

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Section: Screening Of Potential Pectinase Production By Ten Promisingsupporting

confidence: 94%

Retting and degumming of flax using biotechnology eco-friendly approach

Moawad¹,

El‐Rahim

Hashem

et al. 2019

Egypt. J. Chem.

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“…In addition to that, the three isolates of the genus Stenotrophomonas isolated from healthy tomato plants were able to produce indole-3acetic acid; two of these strains had phosphate solubilization ability (Ben Abdallah et al, 2018). S. maltophilia P9 was isolated from the algal biomass and identified as a potential pectinase producing with biotechnological significance (Sharma and Sharma, 2018). In a study, Woźniak et al (2019) reported that Stenotrophomonas strain ES2 promotes growth under in vitro conditions.…”

Section: Plant Growth Promoting Activity Of Stenotrophomonas Sppmentioning

confidence: 99%

Stenotrophomonas in diversified cropping systems: friend or foe?

Kumar,

Rithesh,

Kumar

et al. 2023

Front. Microbiol.

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In the current scenario, the use of synthetic fertilizers is at its peak, which is an expensive affair, possesses harmful effects to the environment, negatively affecting soil fertility and beneficial soil microfauna as well as human health. Because of this, the demand for natural, chemical-free, and organic foods is increasing day by day. Therefore, in the present circumstances use of biofertilizers for plant growth-promotion and microbe-based biopesticides against biotic stresses are alternative options to reduce the risk of both synthetic fertilizers and pesticides. The plant growth promoting rhizobacteria (PGPR) and microbial biocontrol agents are ecologically safe and effective. Owning their beneficial properties on plant systems without harming the ecosystem, they are catching the widespread interest of researchers, agriculturists, and industrialists. In this context, the genus Stenotrophomonas is an emerging potential source of both biofertilizer and biopesticide. This genus is particularly known for producing osmoprotective substances which play a key role in cellular functions, i.e., DNA replication, DNA-protein interactions, and cellular metabolism to regulate the osmotic balance, and also acts as effective stabilizers of enzymes. Moreover, few species of this genus are disease causing agents in humans that is why; it has become an emerging field of research in the present scenario. In the past, many studies were conducted on exploring the different applications of Stenotrophomonas in various fields, however, further researches are required to explore the various functions of Stenotrophomonas in plant growth promotion and management of pests and diseases under diverse growth conditions and to demonstrate its interaction with plant and soil systems. The present review discusses various plant growth and biocontrol attributes of the genus Stenotrophomonas in various food crops along with knowledge gaps. Additionally, the potential risks and challenges associated with the use of Stenotrophomonas in agriculture systems have also been discussed along with a call for further research in this area.

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