Molecular identification of yeasts from the order Trichosporonales causing superficial infections

Martínez‐Herrera, Erick; Duarte-Escalante, Esperanza; Reyes-Montes, María del Roció; Arenas, Roberto; Acosta-Altamirano, Gustavo; Moreno‐Coutiño, Gabriela; Vite-Garín, Tania; Meza-Robles, Alejandro; Frías-De-León, María Guadalupe

doi:10.1016/j.riam.2021.01.004

Cited by 6 publications

(3 citation statements)

References 23 publications

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“…Trichosporonales consists of a diverse group of members involved in fermentation processes and biotransformation of biarylic compounds (Takashima et al 2019 ; Martínez-Herrera et al 2021 ). Saccharomycetales, a common group in maize, were only present in the MRI sample (Sun et al 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Analysis of the bacterial and fungal populations in South African sorghum beer (umqombothi) using full-length 16S rRNA amplicon sequencing

Hlangwani,

Abrahams,

Masenya

et al. 2023

World J Microbiol Biotechnol

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There is a need to profile microorganisms which exist pre-and-post-production of umqombothi, to understand its microbial diversity and the interactions which subsequently influence the final product. Thus, this study sought to determine the relative microbial abundance in umqombothi and predict the functional pathways of bacterial and fungal microbiota present. Full-length bacterial 16S rRNA and internal transcribed spacer (ITS) gene sequencing using PacBio single-molecule, real-time (SMRT) technology was used to assess the microbial compositions. PICRUSt2 was adopted to infer microbial functional differences. A mixture of harmful and beneficial microorganisms was observed in all samples. The microbial diversity differed significantly between the mixed raw ingredients (MRI), customary beer brew (CB), and optimised beer brew (OPB). The highest bacterial species diversity was observed in the MRI, while the highest fungal species diversity was observed in the OPB. The dominant bacterial species in the MRI, CB, and OPB were Kosakonia cowanii, Apilactobacillus pseudoficulneus, and Vibrio alginolyticus, respectively, while the dominant fungal species was Apiotrichum laibachii. The predicted functional annotations revealed significant (p < 0.05) differences in the microbial pathways of the fermented and unfermented samples. The most abundant pathways in the MRI were the branched-chain amino acid biosynthesis super pathway and the pentose phosphate pathway. The CB sample was characterised by folate (vitamin B9) transformations III, and mixed acid fermentation. Biotin (vitamin B7) biosynthesis I and l-valine biosynthesis characterised the OPB sample. These findings can assist in identifying potential starter cultures for the commercial production of umqombothi. Specifically, A. pseudoficulneus can be used for controlled fermentation during the production of umqombothi. Likewise, the use of A. laibachii can allow for better control over the fermentation kinetics such as carbohydrate conversion and end-product characteristics, especially esters and aroma compounds.

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Section: Discussionmentioning

confidence: 99%

Analysis of the bacterial and fungal populations in South African sorghum beer (umqombothi) using full-length 16S rRNA amplicon sequencing

Hlangwani,

Abrahams,

Masenya

et al. 2023

World J Microbiol Biotechnol

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“…The prevalence of non-T. asahii species exhibits geographical differences, T. asteroides, T. inkin and T. faecale being frequently reported. In addition, T. inkin is the most prevalent agent in superficial infections [2,8,9,35].…”

Section: Epidemiology Prevention and Risk Groups Epidemiologymentioning

confidence: 99%

JMM Profile: Trichosporon yeasts: from superficial pathogen to threat for haematological-neutropenic patients

Francisco

Hagen

2022

Journal of Medical Microbiology

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Trichosporon yeasts are classical agents of superficial mycoses, and they are ranked as the first to second predominant basidiomycetous yeast able to cause invasive infections. The clinical presentation of Trichosporon infections varies with the affected anatomical site, with fungaemia present in the majority of invasive trichosporonosis cases. Only a limited number of antifungal compounds can be used to treat Trichosporon infections. Azoles are the first choice due to their intrinsic resistance to echinocandins. Better laboratory methods and up-to-date databases of commercial platforms are required to improve identification, susceptibility testing and surveillance of this potentially threating infection.

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“…Entretanto, este patógeno pode ser considerado um fungo oportunista uma vez que é capaz de causar infecções cutâneas, profundas e disseminadas principalmente em pacientes imunossuprimidos (Francisco et al, 2019;Li et al, 2020). A patogenicidade de fungos pertencentes ao gênero Trichosporon pode ser identificada a partir de modelos in vitro, que por sua vez, são capazes de identificar a presença de fungos patogênicos através da reprodução de suas estrututas morfologicas (blastoconídios, hifas, artroconídios) (Li et al, 2020;Martínez-Herrera et al, 2021). Além disso, através dos modelos in vitro é possivel definir a suscetibilidade deste patógenos fungicos à compostos sintéticos e semi-sintéticos (Jesus, 2018).…”

Section: Introductionunclassified

Modelo experimental in vitro de piedra branca e avaliação do perfil de susceptibilidade de Trichosporon frente a derivados de tiofeno-tiosemicarbazona (L10)

Cavalcanti

Falcão

Ferreira³

et al. 2021

RSD

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O objetivo do presente estudo foi avaliar o potencial de patogenicidade in vitro e o perfil de susceptibilidade de isolados de Trichosporon spp. aos derivados de tiofeno-tiosemicarbazona (L10). Inicialmente, foi realizada uma revisão taxonômica de 3 isolados de Trichosporon spp. de importância clínica estocados na Micoteca URM da Universidade Federal de Pernambuco (UFPE). Posteriormente foi avaliada a capacidade dessas espécies de crescer in vitro em amostras de pelos saudáveis (isentos de tratamentos químicos), a 25 °C e a 37 °C durante um período de 10 dias. Após este período, foi realizado exame direto dos pelos, utilizando KOH a 10%, e cultura em meio Ágar Sabouraud com cloranfenicol das amostras que cresceram no pelo, assim como análises da macro e micromorfologia dessas espécies fúngicas. Por fim, o perfil de susceptibilidade in vitro dessas espécies fúngicas aos derivados de tiofeno-tiosemicarbazona (L10) de acordo com o Clinical and Laboratory Standards Institute (CLSI) foi realizado para determinar as concentrações inibitórias mínimas (CIMs) e as concentrações fungicidas mínimas (CFMs). Através do modelo experimental in vitro foi possível observar que as seguintes espécies: Trichosporon asahii, T. ovoides e T. cutaneum são agentes etiológicos da piedra branca e apresentam potencial patogênico mesmo em um modelo experimental in vitro e sem a necessidade de sinergismo com outros tipos de microrganismos. Através da análise do perfil de susceptibilidade in vitro foi possível observar que os novos derivados de tiofeno testados não são eficazes em combater o crescimento das espécies de Trichosporon avaliadas, sendo assim inadequados para o tratamento das manifestações clínicas observadas na piedra branca. Em conclusão, esse estudo mostrou que é possível cultivar Trichosporon em um modelo in vitro e que são necessárias buscas por outras abordagens terapêuticas eficazes para o combate de infecções causadas pelos fungos do gênero Trichosporon.

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Molecular identification of yeasts from the order Trichosporonales causing superficial infections

Cited by 6 publications

References 23 publications

Analysis of the bacterial and fungal populations in South African sorghum beer (umqombothi) using full-length 16S rRNA amplicon sequencing

Analysis of the bacterial and fungal populations in South African sorghum beer (umqombothi) using full-length 16S rRNA amplicon sequencing

JMM Profile: Trichosporon yeasts: from superficial pathogen to threat for haematological-neutropenic patients

Modelo experimental in vitro de piedra branca e avaliação do perfil de susceptibilidade de Trichosporon frente a derivados de tiofeno-tiosemicarbazona (L10)

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