Molecular evolution of the pDo500 satellite DNA family in Dolichopoda cave crickets (Rhaphidophoridae)

Abstract

BackgroundNon-coding satellite DNA (satDNA) usually has a high turn-over rate frequently leading to species specific patterns. However, some satDNA families evolve more slowly and can be found in several related species. Here, we analyzed the mode of evolution of the pDo500 satDNA family of Dolichopoda cave crickets. In addition, we discuss the potential of slowly evolving satDNAs as phylogenetic markers.ResultsWe sequenced 199 genomic or PCR amplified satDNA repeats of the pDo500 family from 12 Dolichopoda species. For the 38 populations under study, 39 pDo500 consensus sequences were deduced. Phylogenetic analyses using Bayesian, Maximum Parsimony, and Maximum Likelihood approaches yielded largely congruent tree topologies. The vast majority of pDo500 sequences grouped according to species designation. Scatter plots and statistical tests revealed a significant correlation between genetic distances for satDNA and mitochondrial DNA. Sliding window analyses showed species specific patterns of variable and conserved regions. The evolutionary rate of the pDo500 satDNA was estimated to be 1.63-1.78% per lineage per million years.ConclusionsThe pDo500 satDNA evolves gradually at a rate that is only slightly faster than previously published rates of insect mitochondrial COI sequences. The pDo500 phylogeny was basically congruent with the previously published mtDNA phylogenies. Accordingly, the slowly evolving pDo500 satDNA family is indeed informative as a phylogenetic marker.

A new taxonomic status for Iberoformica (Hymenoptera, Formicidae) based on the use of molecular markers

“…Previously, several molecular studies have demonstrated that monomer sequences indicate phylogenies similar to those found with classical markers, and particularly with ribosomal markers, as both exhibit concerted evolution (e.g. Pons and Gillespie 2004;Palomeque and Lorite 2008;Martinsen et al 2009). The consequence of the concerted evolution is an intraspecific variability lower than the inter-specific divergence.…”

Isolation and characterization of two satellite DNAs in some Iberian rock lizards (Squamata, Lacertidae)

“…Unfortunately, very few examples are found in the literature with both fast‐evolving and slow‐evolving satDNAs found within the same species. For instance, in the genus Dolichopoda , a comparison among three satDNA families showed a trend of sequence variability and copy number being positively correlated, and a trend of sequence variability and length of repeats being negatively correlated (Martinsen et al, ). Like in Dolichopoda , it seems that also in the studied lizards an increase in copy number is linked to a trend of sequence homogenization.…”

Comparative phylogeography of two codistributed subgenera of cave crickets (Orthoptera: Rhaphidophoridae: Ceuthophilus spp.)

“…Within the Rhaphidophoridae, both Martinsen et al . () and Allegrucci et al . () found that both vicariance and dispersal were important factors in southern Europe, and similar patterns of variation in geographic genetic structuring of rhaphidophorids previously has also been documented in the United States (Caccone & Sbordoni, ).…”

“…Cavernicoles span a range of ecological adaptations to cave living-troglobites, troglophiles, and trogloxenes-and a concomitant difference in degree of genetic isolation or speciation due to a combination of factors including vicariance and dispersal (Porter, 2007), which may include isolation of cavernous rock units and reduced dispersal capacity across harsh surface habitats (Barr, 1967;Caccone, 1985). Within the Rhaphidophoridae, both Martinsen et al (2009) and Allegrucci et al (2011) found that both vicariance and dispersal were important factors in southern Europe, and similar patterns of variation in geographic genetic structuring of rhaphidophorids previously has also been documented in the United States (Caccone & Sbordoni, 1987). Our findings suggest that for the two codistributed raphidophorid subgenera in the caves of central Texas, colonization occurred either at different times or in different ways, a finding consistent with Allegrucci et al's (2009Allegrucci et al's ( , 2011 Both Ceuthophilus and Geotettix exhibit strong geographic structure in their phylogenies.…”

“…We used mitochondrial DNA sequences from two genes, cytochrome oxidase I (COI) and nicotinamide adenine dinucleotide dehydrogenase subunit 5 (ND5) to reconstruct phylogeographical history of codistributed cave associated species from these two subgenera, because mtDNA evolves rapidly over relatively short timescales and has been particularly useful for comparing levels of population genetic structure in other cavernicoles (e.g. Caccone & Sbordoni, 2001;Martinsen et al, 2009;Bryson et al, 2014). We compare the level of genetic structure between these two cricket subgenera to assess the scale at which differences in dispersal ability might translate into differences in genetic structure.…”