Molecular evidence of Borrelia burgdorferi sensu lato in patients in Brazilian central-western region

Abstract

We aimed to detect DNA of Borrelia burgdorferi in whole blood and serum samples of patients with clinical symptoms and epidemiology compatible with Brazilian Lyme-like disease. Four patients with positive epidemiological histories were recruited for the study. Blood samples were collected, screened by serologic testing by ELISA and Western blotting and molecular identification of B. burgdorferi by amplifying a fragment of the conserved gene that synthesizes the hook flagellar flgE. The results showed positive serology and for the first time, the presence of B. burgdorferi sensu lato in humans in the Midwest region of Brazil. The resulting sequences were similar to GenBank corresponding sequences of B. burgdorferi flgE gene. By neighbor-joining the phylogenetic analysis, the flgE sequence of the Brazilian strain clustered in a monophyletic group with the sequence of B. burgdorferi sensu lato under 100% bootstrap support. This study opens up promising perspectives and reinforces the need for additional studies to determine the epidemiological characteristics of the disease, as well as the impact of the prevalence of Brazilian borreliosis in Mato Grosso do Sul State, Brazil.

Caracterização Das Infecções Relacionadas À Assistência À Saúde Em Uma Unidade De Terapia Intensiva Pediátrica Do Extremo Norte Do Brasil

“…Dessa forma, pode-se observar que na pesquisa realizada na UTIP de Fortaleza, Ceará, a média alcançada foi de 15,52 ± 0,94 dias, sendo assim, muito próximo da encontrada no HCSA (OLIVEIRA, et al, 2017). Ademais, dados obtidos em uma UTI pediátrica indiana, revelam uma média de 14 dias de internação (SODHI et al, 2016).…”

Síndrome de Baggio-Yoshinari: uma revisão da literatura

“…However, there are case reports from 1988, in Rio de Janeiro, there was no description of clinical and laboratory symptoms. Subsequently, some cases were identified in humans and animals, by serological methods in Espírito Santo, Rio de Janeiro, São Paulo, Minas Gerais and Mato Grosso do Sul, Amazonas, Tocantins and Paraná, showing a large geographical distribution area for this infection 3,16,23,24 . According to Dall'Agnol et al 22 , from 2009 to 2016, 4.078 suspected cases of BYS were recorded in Brazil.…”

“…From this perspective, it is assumed that the different geographical conditions of Brazil, combined with the great Brazilian biodiversity, mainly the existence of exotic species of ticks and other natural reservoirs, have contributed to the evolutionary adaptation of the pathogen in the country. Corroborating these investigations, it is described that the spirochetes found in the blood of patients with BYS are in their "L" form, characterized by the presence of a deficient cell wall, loss of flagella and with atypical morphology with a vegetative presentation, a characteristic probably resulting from changes due to unfavorable survival conditions 3,6,[9][10][11][12][13][14][15] .…”

“…B aggio-Yoshinari Syndrome (BYS), also known as Brazilian Lyme-like disease, Lyme borreliosis or Brazilian borreliosis 1,2 , is an emerging Brazilian zoonosis with the first case report was dated in 1992, in the city of São Paulo, Brazil [3][4][5] . It is similar to Lyme disease (LD), a typical disease in the northern hemisphere, presenting all the classic clinical manifestations of LD, but it differs in terms of the frequency of autoimmune episodes and events [6][7][8] .…”

“…Concerning the laboratory alterations, the production of antibodies against autologous cellular components produced during the clinical evolution of the disease is noted, nevertheless, there are still few studies that address this theme 1,3 . The main changes reported by Costa and Yoshinari 7 refer to the presence of ACF (anti-core factor), anti-Ro/La, anti-cardiolipin IgG/IgM, anti-neutrophil cytoplasm, neuronal and cutaneous anti-constituent antibodies, hypergammaglobulinemia and, in some cases, the elevation of IgE.…”