Molecular Evidence for Cryptic Speciation in the Cyclophorus fulguratus (Pfeiffer, 1854) Species Complex (Caenogastropoda: Cyclophoridae) with Description of New Species

Nantarat, Nattawadee; Wade, Christopher M.; Jeratthitikul, Ekgachai; Sutcharit, Chirasak; Panha, Somsak

doi:10.1371/journal.pone.0109785

Cited by 23 publications

(41 citation statements)

References 43 publications

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“…In this study, results suggested that the two species could be unequivocally 274 regarded as separate. In short, the findings of this study are in line with past research in this 275 region which proposed that a combination of morphology and molecular approaches could 276 improve taxonomy of land snails (Nantarat et al, , 2014bLiew et al, 2009;Liew et al, 277 2014). However, we aware that this findings still need to be verified with more specimens of 278 both species from their entire distribution range outside of Sabah.…”

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confidence: 83%

“…Shell colour patterns are usually used as one of the key determinants to discriminate 307 between species in traditional taxonomic classification. One of the sister taxa of Leptopoma, the 308 species in genus Cyclophorus, was distinguished unambiguously based on shell patterns that 309 were also supported by molecular data (Nantarat et al, 2014b). Compared to genus Cyclophorus, 310 shell colour patterns of the L. sericatum and L. pellucidum, exhibited a weak phylogenetic signal.…”

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confidence: 89%

“…Significant inter-and intra-specific variations in quantitative shell characters have been 324 noted both in family Cyclophoridae (Lee et al, 2012;Nantarat et al, 2014b) and in other 325 gastropods (Kameda et al, 2007;Desouky & Busais, 2012;Hirano et al, 2014). In Vermeulen's 326 (1999) descriptions of L. sericatum and L. pellucidum, the ratio of shell height to width of L. 327 sericatum is slightly smaller than L. pellucidum.…”

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confidence: 99%

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Peer Review #1 of "Land snails of Leptopoma Pfeiffer, 1847 in Sabah, Northern Borneo (Caenogastropoda: Cyclophoridae): an analysis of molecular phylogeny and geographical variations in shell form (v0.1)"

2017

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Leptopoma is a species rich genus with approximately 100 species documented. Specieslevel identification in this group has been based on shell morphology and colouration, as well as some anatomical features based on small sample sizes. However, the implications of the inter-and intra-species variations in shell form to the taxonomy of Leptopoma species and the congruency of its current shell based taxonomy with its molecular phylogeny are still unclear. There are four Leptopoma species found in Sabah, Borneo, and their taxonomy status remains uncertain due to substantial variation in shell forms. This study focuses on the phylogenetic relationships and geographical variation in shell form of three Leptopoma species from Sabah.The phylogenetic relationship of these species was first estimated by performing Maximum Likelihood and Bayesian analysis based on mitochondrial genes (16S and COI) and nuclear gene (ITS-1). Then, a total of six quantitative shell characters (i.e. shell height, shell width, aperture height, aperture width, shell spire height, and ratio of shell height to width) and three qualitative shell characters (i.e. shell colour patterns, spiral ridges, and dark apertural band) of the specimens were mapped across the phylogenetic tree and tested for phylogenetic signals. Data on shell characters of Leptopoma sericatum and Leptopoma pellucidum from two different locations (i.e. Balambangan Island and Kinabatangan) where both species occurred sympatrically were then obtained to examine the geographical variations in shell form. The molecular phylogenetic analyses suggested that each of the three Leptopoma species was monophyletic and indicated congruence with only one of the shell characters (i.e. shell spiral ridges) in the current morphological-based classification. Although the geographical variation analyses suggested some of the shell characters indicating inter-species differences between the two Leptopoma species, these also pointed to intra-species differences between populations from different locations. This study on Leptopoma species is based on small sample size and the findings appear only applicable to Leptopoma species in Sabah. Nevertheless, we anticipate this study to be a starting point for more PeerJ reviewing PDF |

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confidence: 89%

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confidence: 99%

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Peer Review #1 of "Land snails of Leptopoma Pfeiffer, 1847 in Sabah, Northern Borneo (Caenogastropoda: Cyclophoridae): an analysis of molecular phylogeny and geographical variations in shell form (v0.1)"

2017

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“…The current classification of many Cyclophorids was based solely on morphology characteristics where its reliability remains doubtful as this taxa possesses exceptionally diverse variations in morphology. Past research has shown that molecular phylogenetic analyses could provide insights into the taxonomy of morphologically-ill land snails in this region (Nantarat et al, 2014a(Nantarat et al, , 2014bLiew et al, 2009;Liew et al, 2014). This study presents the first molecular phylogeny investigation on genus Leptopoma in Sabah, one of the understudied taxa within Cyclophoridae, and examines the concordance between morphology and phylogeny as well as geographical variations in shell form.…”

Section: Discussionmentioning

confidence: 96%

Land snails of Leptopoma Pfeiffer, 1847 in Sabah, Northern Borneo (Caenogastropoda: Cyclophoridae): an analysis of molecular phylogeny and variations in shell form due to geography

Phung

Heng

Liew

2017

Preprint

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Leptopoma is a species rich genus with approximately 100 species documented according to shell morphology and animal anatomy. Many of the Leptopoma species are described in terms of shell size, shape, sculpture and colour patterns of a small number of examined materials. However, the implications of the inter-and intra-species variations in shell form to the taxonomy of Leptopoma species and the congruency of its current shell based taxonomy with its molecular phylogeny are still unclear. Over the last decade, more than 900 collection lots consisting of more than 4000 Leptopoma specimens have been obtained in Sabah and deposited in BORNEENSIS at Universiti Malaysia Sabah. Access to this collection gave us the opportunity to examine the geographical variations in shell forms and the phylogenetic relationship of Leptopoma species in Sabah. The phylogenetic relationship of three Leptopoma species was first estimated by performing maximum likelihood and Bayesian analysis based on mitochondrial genes (16S and COI) and nuclear gene (ITS-1). After this, a total of six quantitative shell characters (i.e. shell height, shell width, aperture height, aperture width, shell spire height, and ratio of shell height and width) and three qualitative shell characters (i.e. shell colour patterns, spiral ridges, and dark ring band in aperture) of the specimens were mapped across the phylogenetic tree two Leptopoma species, these also pointed to intra-species differences between populations from different locations. This study provides an initiation to resolve the taxonomy conundrum for the remaining 100 little known Leptopoma species from other regions and highlights a need to assess variations in shell characters before they could be used in species classification. 8 Abstract. Leptopoma is a species rich genus with approximately 100 species documented 9 according to shell morphology and animal anatomy. Many of the Leptopoma species are described 10 in terms of shell size, shape, sculpture and colour patterns of a small number of examined 11 materials. However, the implications of the inter-and intra-species variations in shell form to the 12 taxonomy of Leptopoma species and the congruency of its current shell based taxonomy with its 13 molecular phylogeny are still unclear. Over the last decade, more than 900 collection lots 14 consisting of more than 4000 Leptopoma specimens have been obtained in Sabah and deposited in 15 BORNEENSIS at Universiti Malaysia Sabah. Access to this collection gave us the opportunity to 16 examine the geographical variations in shell forms and the phylogenetic relationship of Leptopoma 17 species in Sabah. The phylogenetic relationship of three Leptopoma species was first estimated by 18 performing maximum likelihood and Bayesian analysis based on mitochondrial genes (16S and 19 COI) and nuclear gene (ITS-1). After this, a total of six quantitative shell characters (i.e. shell 20 height, shell width, aperture height, aperture width, shell spire height, and ratio of shell height and 21 width) and three...

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“…Sequence data from 32 additional Cyclophorus specimens and three outgroup taxa (Table S2) were taken from the literature (Nantarat et al, 2014b;Nantarat, Wade, Jeratthitikul, Sutcharit, & Panha, 2014c; see there for locality details of this material). The sequences of the protein-coding COI gene were aligned using CLUSTAL W (Thompson, Higgins, & Gibson, 1994) in BIOEDIT version 7.2.6 (Hall, 1999).…”

Section: Sequence Alignment and Phylogenetic Analysismentioning

confidence: 99%

Competition matters: Determining the drivers of land snail community assembly among limestone karst areas in northern Vietnam

Oheimb

Hirano

et al. 2018

Ecology and Evolution

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The insular limestone karsts of northern Vietnam harbor a very rich biodiversity. Many taxa are strongly associated with these environments, and individual species communities can differ considerably among karst areas. The exact processes that have shaped the biotic composition of these habitats, however, remain largely unknown. In this study, the role of two major processes for the assembly of snail communities on limestone karsts was investigated, interspecific competition and filtering of taxa due to geographical factors. Communities of operculate land snails of the genus Cyclophorus were studied using the dry and fluid‐preserved specimen collections of the Natural History Museum, London. Phylogenetic distances (based on a Bayesian analysis using DNA sequence data) and shell characters (based on 200 semilandmarks) were used as proxies for ecological similarity and were analyzed to reveal patterns of overdispersion (indicating competition) or clustering (indicating filtering) in observed communities compared to random communities. Among the seven studied karst areas, a total of 15 Cyclophorus lineages were found. Unique communities were present in each area. The analyses revealed phylogenetic overdispersion in six and morphological overdispersion in four of seven karst areas. The pattern of frequent phylogenetic overdispersion indicated that competition among lineages is the major process shaping the Cyclophorus communities studied. The Coastal Area, which was phylogenetically overdispersed, showed a clear morphological clustering, which could have been caused by similar ecological adaptations among taxa in this environment. Only the community in the Cuc Phuong Area showed a pattern of phylogenetic clustering, which was partly caused by an absence of a certain, phylogenetically very distinct group in this region. Filtering due to geographical factors could have been involved here. This study shows how museum collections can be used to examine community assembly and contributes to the understanding of the processes that have shaped karst communities in Vietnam.

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Molecular Evidence for Cryptic Speciation in the Cyclophorus fulguratus (Pfeiffer, 1854) Species Complex (Caenogastropoda: Cyclophoridae) with Description of New Species

Cited by 23 publications

References 43 publications

Peer Review #1 of "Land snails of Leptopoma Pfeiffer, 1847 in Sabah, Northern Borneo (Caenogastropoda: Cyclophoridae): an analysis of molecular phylogeny and geographical variations in shell form (v0.1)"

Peer Review #1 of "Land snails of Leptopoma Pfeiffer, 1847 in Sabah, Northern Borneo (Caenogastropoda: Cyclophoridae): an analysis of molecular phylogeny and geographical variations in shell form (v0.1)"

Land snails of Leptopoma Pfeiffer, 1847 in Sabah, Northern Borneo (Caenogastropoda: Cyclophoridae): an analysis of molecular phylogeny and variations in shell form due to geography

Competition matters: Determining the drivers of land snail community assembly among limestone karst areas in northern Vietnam

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