Molecular epidemiology of enterovirus outbreaks in Canada during 1991–1992: Identification of echovirus 30 and coxsackievirus B1 strains by amplicon sequencing

Abstract

The relatedness of enteroviral isolates associated with two recent outbreaks in Canada was assessed using direct sequencing of amplicons derived from a large portion of the 5' nontranslated region (NTR) of the viral genome. The amplicons of 60 echovirus 30 isolates originating from seven different provinces in 1991 were found to share 99% or greater sequence identity. Recent coxsackievirus B1 isolates characterised in the same manner were identical to each other. When the 5' NTR sequence of these isolates was compared to prototype strains a difference of 11-15% in nucleotide composition was observed. These results indicate that the variability of nucleotide sequence found in 5' NTRs can be utilized to identify rapidly enteroviral strains associated with particular outbreaks and distinguish them from other strains and serotypes.

Enterovirus meningitis in Brazil, 1998–2003

“…This is also important to help increasing the accuracy of clinical diagnosis, eliminating unnecessary laboratory tests [Drebot et al, 1994].…”

Enterovirus infection of the central nervous system of humans: lack of association with chronic neurological disease

“…Biotinylated nested-PCR products were sequenced using a direct solid-phase T7-polymerase-mediated nucleotide sequencing strategy, using either a fluorescein-labelled sequencing primer for analysis with an ALF automated DNA sequencer (Pharmacia) as described previously by Nicholson et al (1995), or an unlabelled sequencing primer and Dyedeoxy terminators (Applied Biosystems) for analysis with a 373 automated DNA sequencer (Applied Biosystems). Sequences thus derived were compared with published enterovirus sequences (Chang et al, 1989;Drebot et al, 1994;Hughes et al, 1989;Dahllund e~ al., 1995;Iizuka et al, 1987;Jenkins et al, 1987;Klump et al, 1990;Kraus et al, 1995;Lindberg et al, 1987;Pulli et al, 1995;P6yry et al, 1994;Romero & Rotbart, 1995;Ryan et al, 1990;Supanaranond et al, 1992;Toyoda et al, 1984;Zhang et at., 1993;Zheng et al, 1995), using DNASIS to estabish the likely serotypic identity of viruses detected by PCR.…”

Laboratory diagnosis and genetic analysis of a family clustering outbreak of aseptic meningitis due to echovirus 30

“…[5][6][7] In China, outbreaks of aseptic meningitis associated with E30 have been sequentially reported in Jiangsu and Shandong in recent years. 8,9 In our prior study, we found that E30 was the predominant serotype in enterovirus-associated encephalitis from 2010 to 2012.…”