Molecular epidemiology of Brazilian pseudorabies viral isolates

Fonseca, Antônio Augusto; Camargos, Marcelo Fernandes; Oliveira, Anapolino Macedo de; Zanella, J. R. C.; Patrício, Maria A. Carvalho; Braga, Alexandre C.; Cunha, Eliane S.; Dambros, Régia Maria Feltrin; Heinemann, Marcos Bryan; Leite, Rômulo Cerqueira; Reis, Jenner Karlisson Pimenta dos

doi:10.1016/j.vetmic.2009.09.018

Cited by 45 publications

(53 citation statements)

References 32 publications

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“…Clinical samples used were brains of domestic swine positive for PR and were described in a previous experiment (FONSECA JR et al, 2010b). DNA was extracted using phenol/chloroform method according to SAMBROOK et al (2001).…”

Section: Methodsmentioning

confidence: 99%

Different methods of real-time PCR for detection of pseudorabies virus

et al. 2017

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Pseudorabies (PR) is a highly contagious viral disease of great animal health and economic importance in swine industry. The aim of this study was to evaluate different genomic regions, real-time PCR chemistries and equipment for the molecular diagnosis of PR. Eight primer pairs targeting four genes (gB, gC, gE, gD), three different qPCR chemistries (SybrGreen, hydrolysis probes and plexor) and two equipment (ABI7500, Rotorgene 3000) were evaluated. Oligonucleotides targeting gB using hydrolysis probes showed the best performance after evaluating efficiency (99%), the detection limit (10-1.5 TCID50 mL-1) and diagnostic sensitivity and; therefore, those primers were selected for performance verification factors such as repeatability, reproducibility and robustness (1.39% variance between days, 24% variance between analysts and 4.07% variance in analysis error). The qPCR standardized and validated in this research proved to be reliable for the diagnosis of PR and may be used in diagnostic laboratories that follow ISO 17025 and ISO 16140.

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Section: Methodsmentioning

confidence: 99%

Different methods of real-time PCR for detection of pseudorabies virus

et al. 2017

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“…These tools were used to analyze outbreaks occurring in areas of high swine production in Brazil between 1983 and 2003 (9) and in the United States in 1989 (10). Another relevant analysis is the genetic profiling of the strains circulating in wild boars or pigs (10,12) or the investigation of the spread of live vaccine strains among feral pigs (12).…”

Section: Glycoprotein C Sequences Of Pseudorabies Virusmentioning

confidence: 99%

“…SuHV-1 is classified into different genotypes based on restriction endonuclease analysis (3), but more recent studies conducted in Brazil, the United States and Europe have characterized viral strains based on partial sequencing of UL44 focusing on the region encoding the N-terminal portion of glycoprotein C (10,9,20,12). The UL44 gene is one of the most variable regions of the SuHV-1 genome (17).…”

Section: Introductionmentioning

confidence: 99%

Pseudorabies virus can be classified into five genotypes using partial sequences of UL44

Fonseca¹,

Camargos²,

Sales³

et al. 2012

Braz. J. Microbiol.

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Suid herpesvirus 1 (SuHV-1) is the causative agent of pseudorabies (PR), a disease of great importance due to the huge losses it causes in the swine industry. The aim of this study was to determine a method for genotyping SuHV-1 based on partial sequences of the gene coding for glycoprotein C (gC) and to elucidate the possible reasons for the variability of this region. A total of 109 gCsequences collected from GenBank were divided into five major groups after reconstruction of a phylogenetic tree by Bayesian inference. The analysis showed that a portion of gC (approximately 671 bp) is under selective pressure at various points that coincide with regions of protein disorder. It was also possible to divide SuHV-1 into five genotypes that evolved under different selective pressures. These genotypes are not specific to countries or continents, perhaps due to multiple introduction events related to the importation of swine.

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“…Restriction fragment length polymorphism (RFLP) analysis has been used for genomic characterization of PRV, revealing three major genetically distinct PRV groups, or genomic types. More recently, investigators have used UL44 gene (gC gene, encoding glycoprotein C) sequencing for molecular characterization of PRV, introducing the concept of phylogenetic clusters to classify viral strains (Fonseca et al 2010(Fonseca et al , 2012Serena et al 2011;Sozzi et al 2013).…”

mentioning

confidence: 99%