Molecular epidemiology of American trypanosomiasis in Brazil based on dimorphisms of rRNA and mini-exon gene sequences

Usefulness of PCR-based assays to assess drug efficacy in Chagas disease chemotherapy: value and limitations

“…Consequently, genetic strain differences may influence parasitaemia in man and partially explain the discrepancies of PCR sensitivity between studies carried out in different endemic areas. For instance, in Bolivia and most part of Brazil, in areas where T. cruzi II stocks circulate, PCR shows high sensitivity, contrasting with areas from the Brazilian Amazon and Mexico, where only T. cruzi I stocks occur (Avila et al 1993, Wincker et al 1994a, b, 1997, Zingales et al 1998, Fernandes et al 2001.…”

Programa de Controle da Doença de Chagas no Estado de São Paulo: sorologia de moradores como parte de investigação de unidades domiciliares com presença de triatomíneos vetores na década de 1990

“…cruzi pudessem ser analisados quanto à relação parasita-hospedeiro. Deveriam ser então usadas técnicas de aquisição recente (Diotaiuti et al, 1995;Fernandes et al, 1997;Zingales et al, 1998), para possibilitar a identificação de possíveis elos epidemiológicos entre cepas silvestres e domésticas de Tr. cruzi.…”

Biology and ultra-structure of Trypanosoma cruzi: a 90-years old challenge for scientists

“…Recent reviews from Dr Wanderley De Souza (1984,1995) summarize the knowledge in this area. A large knowledge was obtained on the understanding of its clonal composition (Heckert et al 1994, Tibayrenc 1995, as well as in its population distribution and associated biological and molecular markers (Zingales et al 1998). Another important field on parasite biology investigation concerns its metabolic pathways (reviewed in Cazzulo 1992), that engages the design of new drugs based on differences encountered.…”