Molecular epidemiology and antifungal susceptibility of Candida parapsilosis sensu stricto, Candida orthopsilosis, and Candida metapsilosis in Taiwan

Chen, Yee‐Chun; Lin, Yu-Hsin; Chen, Kuo-Wei; Lii, Joanne; Teng, Hwa-Jen; Li, Shuying

doi:10.1016/j.diagmicrobio.2010.07.004

Cited by 47 publications

(51 citation statements)

References 48 publications

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“…This suggests that C. parapsilosis may have undergone a population bottleneck and that all global isolates are closely related. C. parapsilosis is much more commonly isolated from clinical samples than either C. metapsilosis or C. orthopsilosis (15,43,66). Our analysis shows that the degeneration of MTLa1 is a recent event in C. parapsilosis, because the gene is intact in C. orthopsilosis.…”

Section: Discussionmentioning

confidence: 70%

Evolution of Mating within the Candida parapsilosis Species Group

et al. 2011

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Candida orthopsilosis and Candida metapsilosis are closely related to Candida parapsilosis, a major cause of infection in premature neonates. Mating has not been observed in these species. We show that ϳ190 isolates of C. parapsilosis contain only an MTLa idiomorph at the mating-type-like locus. Here, we describe the isolation and characterization of the MTL loci from C. orthopsilosis and C. metapsilosis. Among 16 C. orthopsilosis isolates, 9 were homozygous for MTLa, 5 were homozygous for MTL␣, and 2 were MTLa/␣ heterozygotes. The C. orthopsilosis isolates belonged to two divergent groups, as characterized by restriction patterns at MTL, which probably represent subspecies. We sequenced both idiomorphs from each group and showed that they are 95% identical and that the regulatory genes are intact. In contrast, 18 isolates of C. metapsilosis contain only MTL␣ idiomorphs. Our results suggest that the role of MTL in determining cell type is being eroded in the C. parapsilosis species complex. The population structure of C. orthopsilosis indicates that mating may occur. However, expression of genes in the mating signal transduction pathway does not respond to exposure to alpha factor. C. parapsilosis is also nonresponsive, even when the GTPase-activating protein gene SST2 is deleted. In addition, splicing of introns in MTLa1 and MTLa2 is defective in C. orthopsilosis. Mating is not detected. The alpha factor peptide, which is the same sequence in C. parapsilosis, C. orthopsilosis, and C. metapsilosis, can induce a mating response in Candida albicans. It is therefore likely either that mating of C. orthopsilosis takes place under certain unidentified conditions or that the mating pathway has been adapted for other functions, such as cross-species communication.

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Section: Discussionmentioning

confidence: 70%

Evolution of Mating within the Candida parapsilosis Species Group

et al. 2011

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“…In Portugal and Denmark, analogous proportions of C. orthopsilosis and C. metapsilosis isolates were also detected (Silva et al, 2009;Mirhendi et al, 2010). Chen et al (2010) described one of the highest rates of C. metapsilosis occurrence (4.4 %) among 45 nonduplicating bloodstream isolates from Taiwan. In Malaysia, higher percentages of C. orthopsilosis (23.8 %) and C. metapsilosis (4.8 %) have been described (Tay et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Although C. parapsilosis sensu stricto is not considered to be a resistant species (Gomez-Lopez et al, 2008;Lockhart et al, 2008;van Asbeck et al, 2008;Tay et al, 2009;Chen et al, 2010), previous reports have suggested that both C. orthopsilosis and C. metapsilosis possess amphotericin B and caspofungin MICs that are lower than those for C. parapsilosis but have slightly higher fluconazole MICs overall than C. parapsilosis (Lockhart et al, 2008;Silva et al, 2009;Pfaller et al, 2011). As described by van Asbeck et al (2008), the distinct affinity of azoles for the key ergosterolsynthesizing enzyme 14-demethylase, or other enzymes involved in this metabolic pathway, could explain the differences in susceptibility to voriconazole and fluconazole among the three species.…”

Section: Discussionmentioning

confidence: 99%

Prevalence, distribution and antifungal susceptibility profiles of Candida parapsilosis, Candida orthopsilosis and Candida metapsilosis bloodstream isolates

Bonfietti

Martins

Szeszs

et al. 2012

Journal of Medical Microbiology

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“…Similarly, FLC resistance in C. metapsilosis and C. parapsilosis sensu stricto has been observed by Chen et al (40) Moreover, Gomez-Lopez et al (41) found an MIC of greater than 4µg/mL FLC for these species, while Silva et al (6) found an MIC of 16µg/mL FLC for one C. parapsilosis sensu stricto isolate. According to Van Asbeck et al (42) , the differences in FLC susceptibility may also refl ect the different affi nities of azoles for the key ergosterol-synthesizing enzyme 14-α-demethylase or for other enzymes in this pathway.…”

Section: In Vitro Susceptibility Testingmentioning

confidence: 95%

Molecular identification and antifungal susceptibility profiles of Candida parapsilosis complex species isolated from culture collection of clinical samples

Ataídes

Costa

Souza

et al. 2015

Rev. Soc. Bras. Med. Trop.

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Introduction:Candida parapsilosis is a common yeast species found in cases of onychomycosis and candidemia associated with infected intravascular devices. In this study, we differentiated Candida parapsilosis sensu stricto, Candida orthopsilosis, and Candida metapsilosis from a culture collection containing blood and subungual scraping samples. Furthermore, we assessed the in vitro antifungal susceptibility of these species to fl uconazole, itraconazole, voriconazole, posaconazole, amphotericin B, and caspofungin. Methods: Differentiation of C. parapsilosis complex species was performed by amplifi cation of the secondary alcohol dehydrogenase (SADH) gene and digestion by the restriction enzyme BanI. All isolates were evaluated for the determination of minimal inhibitory concentrations using Etest, a method for antifungal susceptibility testing. Results: Among the 87 isolates, 78 (89.7%) were identifi ed as C. parapsilosis sensu stricto, fi ve (5.7%) were identifi ed as C. orthopsilosis, and four (4.6%) were identifi ed as C. metapsilosis. Analysis of antifungal susceptibility showed that C. parapsilosis sensu stricto isolates were less susceptible to amphotericin B and itraconazole. One C. parapsilosis sensu stricto isolate was resistant to amphotericin B and itraconazole. Moreover, 10.2% of C. parapsilosis sensu stricto isolates were resistant to caspofungin. Two C. parapsilosis sensu stricto isolates and one C. metapsilosis isolate were susceptible to fl uconazole in a dose-dependent manner. Conclusions: We reported the fi rst molecular identifi cation of C. parapsilosis complex species in State of Goiás, Brazil. Additionally, we showed that although the three species exhibited differences in antifungal susceptibility profi les, the primary susceptibility of this species was to caspofungin.

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Molecular epidemiology and antifungal susceptibility of Candida parapsilosis sensu stricto, Candida orthopsilosis, and Candida metapsilosis in Taiwan

Cited by 47 publications

References 48 publications

Evolution of Mating within the Candida parapsilosis Species Group

Evolution of Mating within the Candida parapsilosis Species Group

Prevalence, distribution and antifungal susceptibility profiles of Candida parapsilosis, Candida orthopsilosis and Candida metapsilosis bloodstream isolates

Molecular identification and antifungal susceptibility profiles of Candida parapsilosis complex species isolated from culture collection of clinical samples

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