Molecular dynamics simulation to rationalize regioselective hydroxylation of aromatic substrates by soluble methane monooxygenase

Sigdel, Sujan; Gao, Hui; Smith, Thomas J.; Murrell, J. Colin; Lee, Jung-Kul

doi:10.1016/j.bmcl.2015.01.069

Cited by 11 publications

(3 citation statements)

References 29 publications

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“…In its oxidation mechanism, MMO splits an oxygen molecule into two single oxygen atoms and introduces one of these oxygen atoms into CH 4 . The oxidation of CH 4 into methanol is the first step of the metabolic pathway of methanotrophs [40]. However, methanol is further oxidized to formaldehyde in the metabolic pathway; therefore, high methanol production using methanotrophs remains a challenge.…”

Section: Introductionmentioning

confidence: 99%

Potential of Immobilized Whole-Cell Methylocella tundrae as a Biocatalyst for Methanol Production from Methane

Mardina¹,

Li²,

Patel³

et al. 2016

Journal of Microbiology and Biotechnology

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Methanol is a versatile compound that can be biologically synthesized from methane (CH4) by methanotrophs using a low energy-consuming and environment-friendly process. Methylocella tundrae is a type II methanotroph that can utilize CH4 as a carbon and energy source. Methanol is produced in the first step of the metabolic pathway of methanotrophs and is further oxidized into formaldehyde. Several parameters must be optimized to achieve high methanol production. In this study, we optimized the production conditions and process parameters for methanol production. The optimum incubation time, substrate, pH, agitation rate, temperature, phosphate buffer and sodium formate concentration, and cell concentration were determined to be 24 h, 50% CH4, pH 7, 150 rpm, 30°C, 100 mM and 50 mM, and 18 mg/ml, respectively. The optimization of these parameters significantly improved methanol production from 0.66 to 5.18 mM. The use of alginate-encapsulated cells resulted in enhanced methanol production stability and reusability of cells after five cycles of reuse under batch culture conditions.

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Section: Introductionmentioning

confidence: 99%

Potential of Immobilized Whole-Cell Methylocella tundrae as a Biocatalyst for Methanol Production from Methane

Mardina¹,

Li²,

Patel³

et al. 2016

Journal of Microbiology and Biotechnology

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“…However, Type II methanotrophs, including Methylosinus, Methylocella and Methylocystis, produce both soluble (sMMO) and particulate (pMMO) forms of MMO. The Type X methanotrophs possess certain properties common to both Type I and II methanotrophs [5,29,41].…”

Section: Introductionmentioning

confidence: 99%

Production of Methanol from Methane by Encapsulated Methylosinus sporium

Patel¹,

Jeong²,

Mehariya³

et al. 2016

Journal of Microbiology and Biotechnology

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Massive reserves of methane (CH₄) remain unexplored as a feedstock for the production of liquid fuels and chemicals, mainly because of the lack of economically suitable and sustainable strategies for selective oxidation of CH₄ to methanol. The present study demonstrates the bioconversion of CH₄ to methanol mediated by Type I methanotrophs, such as and. Furthermore, immobilization of a Type II methanotroph, , was carried out using different encapsulation methods, employing sodium-alginate (Na-alginate) and silica gel. The encapsulated cells demonstrated higher stability for methanol production. The optimal pH, temperature, and agitation rate were determined to be pH 7.0, 30°C, and 175 rpm, respectively, using inoculum (1.5 mg of dry cell mass/ml) and 20% of CH₄ as a feed. Under these conditions, maximum methanol production (3.43 and 3.73 mM) by the encapsulated cells was recorded. Even after six cycles of reuse, the Na-alginate and silica gel encapsulated cells retained 61.8% and 51.6% of their initial efficiency for methanol production, respectively, in comparison with the efficiency of 11.5% observed in the case of free cells. These results suggest that encapsulation of methanotrophs is a promising approach to improve the stability of methanol production.

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“…For example, alkane hydroxylations are catalyzed by completely dissimilar enzymes such as methane monooxygenase41, cytochrome P450s (CYP153A642 or CYP52 from Candida 43, alkB from Pseudomonas 44), or fungal peroxygenases45, indicating that convergent evolution is well represented in nature for important biochemical reactions. Plants adopt several defense tactics against herbivory, chemical or mechanical attack.…”

Section: Discussionmentioning

confidence: 99%

Enzyme discovery beyond homology: a unique hydroxynitrile lyase in the Bet v1 superfamily

Lanfranchi

Pavkov‐Keller

Koehler

et al. 2017

Sci Rep

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Homology and similarity based approaches are most widely used for the identification of new enzymes for biocatalysis. However, they are not suitable to find truly novel scaffolds with a desired function and this averts options and diversity. Hydroxynitrile lyases (HNLs) are an example of non-homologous isofunctional enzymes for the synthesis of chiral cyanohydrins. Due to their convergent evolution, finding new representatives is challenging. Here we show the discovery of unique HNL enzymes from the fern Davallia tyermannii by coalescence of transcriptomics, proteomics and enzymatic screening. It is the first protein with a Bet v1-like protein fold exhibiting HNL activity, and has a new catalytic center, as shown by protein crystallography. Biochemical properties of D. tyermannii HNLs open perspectives for the development of a complementary class of biocatalysts for the stereoselective synthesis of cyanohydrins. This work shows that systematic integration of -omics data facilitates discovery of enzymes with unpredictable sequences and helps to extend our knowledge about enzyme diversity.

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Molecular dynamics simulation to rationalize regioselective hydroxylation of aromatic substrates by soluble methane monooxygenase

Cited by 11 publications

References 29 publications

Potential of Immobilized Whole-Cell Methylocella tundrae as a Biocatalyst for Methanol Production from Methane

Potential of Immobilized Whole-Cell Methylocella tundrae as a Biocatalyst for Methanol Production from Methane

Production of Methanol from Methane by Encapsulated Methylosinus sporium

Enzyme discovery beyond homology: a unique hydroxynitrile lyase in the Bet v1 superfamily

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