Molecular Diversity within Chitinolytic Actinomycetes Determined by In Situ Analysis.

Metcalfe, Angela C.; Williamson, Neil R.; Krsek, Martin; Wellington, E. M. H.

doi:10.3209/saj.17_18

Cited by 7 publications

(4 citation statements)

References 24 publications

(28 reference statements)

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“…Actinobacteria are considered as the dominant organisms involved in the decomposition of chitin in soil [1] and also promising antagonist agents for biocontrol due to the hydrolysis reaction over the fungi mycelium [97]. The species that belong to Streptomyces genus are considered as the principal chitinolytic microbial group in soil, due to its capacity to degrade this polymer [98].…”

Section: Chitinases Productionmentioning

confidence: 99%

Actinobacteria as Plant Growth-Promoting Rhizobacteria

Franco-Correa¹,

Chavarro-Anzola²

2016

Actinobacteria - Basics and Biotechnological Applications

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Abstract"ctinobacteria commonly inhabit the rhizosphere, being an essential part of this environment due to their interactions with plants. Such interactions have made possible to characterize them as plant growth-promoting rhizobacteria PGPR . "s PGPR, they possess direct or indirect mechanisms that favor plant growth. "ctinobacteria improve the availability of nutrients and minerals, synthesized plant growth regulators, and specially, they are capable of inhibiting phytopathogens. Different activities that are performed by actinobacteria have been studied, such as phosphate solubilization, siderophores production, and nitrogen fixation. Furthermore, actinobacteria do not contaminate the environment instead, they help to maintain the biotic equilibrium of soil by cooperating with nutrients cycling. The aforementioned is directly related to the quality and productivity of crops. Moreover, different aspects of these microorganisms have been studied, such as production of metabolites that improve plant growth, resilience against unfavorable environmental conditions, and beneficial and synergic interactions with arbuscular mycorrhizal fungi. Taking into account the above-mentioned activities, actinobacteria can be considered as possible plant fertilizers.

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Section: Chitinases Productionmentioning

confidence: 99%

Actinobacteria as Plant Growth-Promoting Rhizobacteria

Franco-Correa¹,

Chavarro-Anzola²

2016

Actinobacteria - Basics and Biotechnological Applications

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“…Streptomycetes are said to be the major chitinovorous microbial groups in soil due to their ability to degrade chitin. This has been long regarded as characteristic feature of a soil Streptomycetes [14]. The organisms which produce chitinase can be used directly in biological control of fungi, or indirectly by the use of purified protein or through the gene manipulation [15].…”

Section: Introductionmentioning

confidence: 99%

Influence of bioprocess variables on the production of extracellular chitinase under submerged fermentation by Streptomyces pratensis strain KLSL55

A.Shivalee

Lingappa

Mahesh

2018

Journal of Genetic Engineering and Biotechnology

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Chitinases are the enzymes which are capable of hydrolyzing chitin to its monomer N-acetyl glucosamine (GlcNac). Present study emphasizes on the impact of critical process variables on the production of chitinase from Streptomyces pratensis strain KLSL55. Initially the isolate was noticed to produce 84.67 IU chitinase in basal production medium. At optimization of bioprocess variables, the physical parameters pH of 8.00, 40 °C of incubation temperature, agitation speed of 160 rpm and 1.25 mL of spore suspension were found optimum for improved production of chitinase. Further, formulated production medium with 1.5% colloidal chitin, 1.25% fructose greatly influenced the chitinase production. At all described optimum conditions with formulated production media, a total of 14.30-fold increment was achieved in the chitinase production with final activity of 1210.67 IU when compared to the initial fermentation conditions in basal production medium.

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“…So far, a few studies have assessed bacterial chitinase gene diversities in different terrestrial and aquatic ecosystems (Hjort et al 2010 ; Hobel et al 2005 ). Also, the biochemical and functional properties (including antibacterial and antifungal activities) of chitinases obtained from specific bacteria isolated from different habitats have been investigated (LeCleir et al 2004 ; Metcalfe et al 2002b ; Williamson et al 2000 ). By using culture-dependent and -independent methods, considerable diversities of chiA genes and predicted catalytic activities were found.…”

Section: Introductionmentioning

confidence: 99%

Mining of unexplored habitats for novel chitinases—chiA as a helper gene proxy in metagenomics

Cretoiu

Kielak

Al‐Soud

et al. 2012

Appl Microbiol Biotechnol

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The main objective of this study was to assess the abundance and diversity of chitin-degrading microbial communities in ten terrestrial and aquatic habitats in order to provide guidance to the subsequent exploration of such environments for novel chitinolytic enzymes. A combined protocol which encompassed (1) classical overall enzymatic assays, (2) chiA gene abundance measurement by qPCR, (3) chiA gene pyrosequencing, and (4) chiA gene-based PCR-DGGE was used. The chiA gene pyrosequencing is unprecedented, as it is the first massive parallel sequencing of this gene. The data obtained showed the existence across habitats of core bacterial communities responsible for chitin assimilation irrespective of ecosystem origin. Conversely, there were habitat-specific differences. In addition, a suite of sequences were obtained that are as yet unregistered in the chitinase database. In terms of chiA gene abundance and diversity, typical low-abundance/diversity versus high-abundance/diversity habitats was distinguished. From the combined data, we selected chitin-amended agricultural soil, the rhizosphere of the Arctic plant Oxyria digyna and the freshwater sponge Ephydatia fluviatilis as the most promising habitats for subsequent bioexploration. Thus, the screening strategy used is proposed as a guide for further metagenomics-based exploration of the selected habitats.Electronic supplementary materialThe online version of this article (doi:10.1007/s00253-012-4057-5) contains supplementary material, which is available to authorized users.

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Molecular Diversity within Chitinolytic Actinomycetes Determined by In Situ Analysis.

Cited by 7 publications

References 24 publications

Actinobacteria as Plant Growth-Promoting Rhizobacteria

Actinobacteria as Plant Growth-Promoting Rhizobacteria

Influence of bioprocess variables on the production of extracellular chitinase under submerged fermentation by Streptomyces pratensis strain KLSL55

Mining of unexplored habitats for novel chitinases—chiA as a helper gene proxy in metagenomics

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