Molecular dissection of Tomato leaf curl virus resistance in tomato line TY172 derived from Solanum peruvianum

Anbinder, Ilana; Reuveni, Moshe; Azari, Raviv; Paran, Ilan; Nahon, Sahadia; Shlomo, Haviva; Chen, Lea; Lapidot, Moshe; Levin, Ilan

doi:10.1007/s00122-009-1060-z

Cited by 150 publications

(114 citation statements)

References 34 publications

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“…With increasing international importance of leaf curl disease in tomatoes, increased efforts have been made to identify resistance to tomato leaf curl disease and incorporate it into improved tomato cultivars (Kenyon et al, 2014). To date, five major genes for resistance or tolerance to TYLCV (Ty-1-Ty-5) have been identified from wild tomato relatives, and some major genes have been incorporated into commercial cultivars in different areas (Anbinder et al, 2009;Hutton et al, 2012;Ji et al, 2007Ji et al, , 2009Lapidot et al, 2015;Verlaan et al, 2013;Zamir et al, 1994). However, whether Ty-1-Ty-5 genes also show resistance or tolerance to local begomovirus species of Southeast Asia is unknown.…”

Section: Introductionmentioning

confidence: 99%

Highly Efficient Agroinoculation Method for Tomato Plants with Tomato Yellow Leaf Curl Kanchanaburi Virus

Koeda¹,

Homma²,

Tanaka³

et al. 2017

The Hortic J

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Tomato yellow leaf curl disease caused by begomoviruses is a serious threat to tomato (Solanum lycopersicum L.) production. If begomoviruses, transmitted by whitefly (Bemisia tabaci), infect tomato plants during early growth, production can be almost entirely lost. Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV), a bipartite Begomovirus, is emerging as an important threat to solanaceous crop production in Southeast Asia. The lack of mechanical transmission of some begomoviruses is a major experimental constraint. In this study, an agroinoculation method using TYLCKaV in tomato plants was established. Partial tandem repeats of TYLCKaV DNA A and DNA B were constructed and cloned to a binary pGreenII vector, and their infectivity was tested. Co-inoculation of TYLCKaV DNA A and DNA B to Nicotiana benthamiana L. produced typical begomoviral symptoms, and both of the viral DNA components accumulated in the upper uninoculated leaves, suggesting systemic infection of TYLCKaV. Two agroinoculation methods were conducted on tomatoes. First, excised sections of tomato shoots were agroinoculated with a soaking procedure. Although two Agrobacterium tumefaciens strains were tested, approximately 40% of inoculated plants only showed viral symptoms for EHA105. Second, agrobacterium from a cultured petri dish was directly inoculated with a colony inoculation procedure. When EHA105 was used, approximately 92% of inoculated plants showed viral symptoms. Sequencing the recovered viral DNA from the upper uninoculated leaf clarified that TYLCKaV had successfully infected the tomato plants. The colony inoculation procedure is labor-saving, and viral symptoms develop in susceptible tomato plants within approximately a month from sowing the seeds. This method could contribute to simple and speedy evaluation of TYLCKaV resistance of tomato plants.

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Section: Introductionmentioning

confidence: 99%

Highly Efficient Agroinoculation Method for Tomato Plants with Tomato Yellow Leaf Curl Kanchanaburi Virus

Koeda¹,

Homma²,

Tanaka³

et al. 2017

The Hortic J

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“…An example is the tomato yellow leaf curl virus (TYLCV) that has been introgressed into the tomato breeding line TY172. The TYLCV resistance in TY172 is controlled by a previously unknown major QTL (Ty-5, mapped to chromosome 4), originating from the resistant line, and four additional minor QTLs (mapped to chromosomes 1, 7, 9 and 11) [28]. Several accessions of S. peruvianum have been identified with resistance to root-knot nematode controlled by Mi gene located on chromosome six [29].…”

Section: Resultsmentioning

confidence: 99%

Relative Response of Four Tomato Species to Rotylenchulus reniformis Infestation

McEwan¹,

Kantety²,

Nyaku³

et al. 2014

AJPS

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The reniform nematode (Rotylenchulus reniformis) is among the most economically damaging plant pathogens in the United States. This nematode is mostly known for its damage to cotton but tomato is also well-within its vast host range that includes 314 plant species across 77 plant families. Nematode-resistant genotypes offer an effective, environmentally safe alternative to agro-chemicals for reniform nematode management. Resistance genes can be introgressed into cultivars through plant improvement efforts. Tomato is a diploid species which is more amenable to identification of resistance genes in contrast to cotton where cultivars are either tetraploid or hexaploid.This greenhouse study examined cultivated and wild Solanum species represented by 40 tomato accessions, to identify resistance and susceptibility responses to R. reniformis. Accessions were evaluated by using single plants in six replicates. Seeds were germinated in sterile soil and inoculated with mixed vermiform R. reniformis. After seven weeks, eggs and vermiform stages were extracted from the root system and counted. A susceptible control S. lycopersicum "Rutgers" (LA1090) was included. Seven putatively resistant tomato genotypes were identified. These genotypes in increasing order of resistance are S. chilense (LA1029), S. lycopersicum (LA1792), S. chilense (LA1932), S. peruvianum var. humifusum (LA0385) S. pimpinellifolium (LA2934), S. peruvianum f. glandulosum (LA1283) and S. pimpinellifolium (LA1579).

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“…Tomato yellow leaf curl virus, Tomato mosaic virus S. chilense [198] Ty-4 Tomato yellow leaf curl virus S. chilense [197] Ty-5 S. peruvianum [199] tcm-1 …”

Section: Tomato Resistance Genes and Their Modes Of Actionmentioning

confidence: 99%

Resistance-Gene-Mediated Defense Responses against Biotic Stresses in the Crop Model Plant Tomato

Chaudhary¹,

Hs²

2017

J Plant Pathol Microbiol

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Complex series of defense response activation, consistent with the studies conducted in the model plant Arabidopsis thaliana, has been demonstrated in tomato during incompatible pathogen/pest interactions. During the past two decades, numerous tomato genes have been identified conferring resistance to diverse pathogens/ pests in a gene-for-gene manner. A few of these cloned resistance (R)-genes (Cf and Pto) have been extensively studied and excellent existing reviews describe R-gene function, interacting proteins and the mechanism of Avirulence effector perception. Recent comprehensive gene expression analysis of tomato responses to biotic stresses resulted in identification of genes and potential molecular processes that are associated with several of the tomato R-genemediated resistance responses. The purpose of this review is to provide an overview of tomato R-gene-mediated defense responses to different pathogens/pests together with the components involved in the organization of this highly complex network of plant defenses. Global transcriptome profiling is an important initial step for dissecting biological systems particularly with systems where not much is known about the molecular basis of the resistance response. The enrichment of the tomato EST databases initiated several genomewide profiling studies [32][33][34][35]. This facilitated significant progress in the characterization of tomato incompatible responses to Avr pathogens, contributing to future gene identification and to the understanding of the potential molecular processes that are associated with the different tomato R-gene-mediated resistances [33]. With the advent of next generation sequencing technologies, and the tomato genome sequence [36], additional genome wide studies have been conducted. With a genome sequence and a high-density linkage and molecular maps [37], combined with being a host for numerous pathogens and pests, tomato has emerged as a powerful model system for crop plant defense response studies. Moreover, the introgression of R-genes from wild species into cultivated tomato provides a unique opportunity to study different resistance mechanisms against very diverse biotic agents in a single plant. In this review, we summarize the current understanding of R-genes in tomato and the downstream signaling components that are critical for activating defense responses. In addition, we discuss the current and future technologies that will significantly enhance our knowledge about tomato-pathogen interactions and will provide alternative strategies to develop a sustainable resistance. Tomato Resistance Genes and their Modes of ActionHost resistance is an important component of a sustainable disease management system [38]. It is an environmentally benign method that can be used as an alternative to chemicals, as their applicability against Verticillium. The Ve locus contains two closely linked and inversely oriented genes, Ve1 and Ve2, both encoding a RLP-type cell surface receptor. Ve1 R-gene provides resistance against ra...

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Molecular dissection of Tomato leaf curl virus resistance in tomato line TY172 derived from Solanum peruvianum

Cited by 150 publications

References 34 publications

Highly Efficient Agroinoculation Method for Tomato Plants with <i>Tomato Yellow Leaf Curl Kanchanaburi Virus</i>

Highly Efficient Agroinoculation Method for Tomato Plants with <i>Tomato Yellow Leaf Curl Kanchanaburi Virus</i>

Relative Response of Four Tomato Species to <i>Rotylenchulus reniformis</i> Infestation

Resistance-Gene-Mediated Defense Responses against Biotic Stresses in the Crop Model Plant Tomato

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Molecular dissection of Tomato leaf curl virus resistance in tomato line TY172 derived from Solanum peruvianum

Cited by 150 publications

References 34 publications

Highly Efficient Agroinoculation Method for Tomato Plants with <i>Tomato Yellow Leaf Curl Kanchanaburi Virus</i>

Highly Efficient Agroinoculation Method for Tomato Plants with <i>Tomato Yellow Leaf Curl Kanchanaburi Virus</i>

Relative Response of Four Tomato Species to &lt;i&gt;Rotylenchulus reniformis&lt;/i&gt; Infestation

Resistance-Gene-Mediated Defense Responses against Biotic Stresses in the Crop Model Plant Tomato

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Relative Response of Four Tomato Species to <i>Rotylenchulus reniformis</i> Infestation