Molecular diagnostics

Bullman, Susan; Lucey, Brigid; Sleator, Roy D.

doi:10.4161/bbug.3.1.19011

Cited by 19 publications

(13 citation statements)

References 55 publications

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“…In 2009 our group established a partnership with Serosep (an Irish molecular diagnostics company) and the Clinical Microbiology Department of Cork University Hospital (CUH), focused on the changing culture of medical microbiology in Ireland. 71 A retrospective analysis of faecal samples submitted to CUH in 2009 was performed to identify all Campylobacter species detected using Serosep's EntericBio Ò multiplex PCR system, in a single calendar year. The results confirmed that routine culture fails to detect over a third of Campylobacter positive samples.…”

Section: Bacterial Diagnosticsmentioning

confidence: 99%

Under the microscope: From pathogens to probiotics and back

Sleator¹

2015

Bioengineered

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Section: Bacterial Diagnosticsmentioning

confidence: 99%

Under the microscope: From pathogens to probiotics and back

Sleator¹

2015

Bioengineered

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“…However, advances in molecular detection systems continue to highlight the fact that routine Campylobacter culture methods, employed by the majority of clinical laboratories, are incapable of detecting the fastidious and non-thermophilic Campylobacter spp [5]. Recent work in our laboratory has focused on the identification and characterisation of these atypical species of potential clinical importance [3,6].…”

Section: Correspondencementioning

confidence: 99%

Molecular-based detection of the gastrointestinal pathogen Campylobacter ureolyticus in unpasteurized milk samples from two cattle farms in Ireland

et al. 2012

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Campylobacter jejuni and coli are collectively regarded as the most prevalent cause of bacterial foodborne illness worldwide. An emerging species, Campylobacter ureolyticus has recently been detected in patients with gastroenteritis, however, the source of this organism has, until now, remained unclear. Herein, we describe the molecular-based detection of this pathogen in bovine faeces (1/20) and unpasteurized milk (6/47) but not in poultry (chicken wings and caeca). This is, to the best of our knowledge, the first report of the presence of this potential gastrointestinal pathogen in an animal source, possibly suggesting a route for its transmission to humans.

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“…The random nature of SCRaMbLE events can also lead to a number of sub-optimal outcomes with regards to studying rearrangements in an unbiased fashion. SCRaMbLE of haploid strains bearing one or more synthetic chromosomes results in a high lethality rate due to the deletion of one or more essential genes 3 , 6 . Additionally, deletion of important but nonessential genes may mask an otherwise apparent change of phenotype.…”

Section: Introductionmentioning

confidence: 99%

Heterozygous diploid and interspecies SCRaMbLEing

Shen

Yang

et al. 2018

Nat Commun

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SCRaMbLE (Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution) is a genome restructuring technique that can be used in synthetic genomes such as that of Sc2.0, the synthetic yeast genome, which contains hundreds to thousands of strategically positioned loxPsym sites. SCRaMbLE has been used to induce rearrangements in yeast strains harboring one or more synthetic chromosomes, as well as plasmid DNA in vitro and in vivo. Here we describe a collection of heterozygous diploid strains produced by mating haploid semisynthetic Sc2.0 strains to haploid native parental strains. We subsequently demonstrate that such heterozygous diploid strains are more robust to the effects of SCRaMbLE than haploid semisynthetic strains, rapidly improve rationally selected phenotypes in SCRaMbLEd heterozygous diploids, and establish that multiple sets of independent genomic rearrangements are able to lead to similar phenotype enhancements. Finally, we show that heterozygous diploid SCRaMbLE can also be carried out in interspecies hybrid strains.

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Molecular diagnostics

Cited by 19 publications

References 55 publications

Under the microscope: From pathogens to probiotics and back

Under the microscope: From pathogens to probiotics and back

Molecular-based detection of the gastrointestinal pathogen Campylobacter ureolyticus in unpasteurized milk samples from two cattle farms in Ireland

Heterozygous diploid and interspecies SCRaMbLEing

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