Molecular detention of efflux pumps in Pseudomonas aeruginosa obtained from different clinical cases

Abstract

150 samples were collected from different clinical sources from Al-Diwaniyah city hospitals from September 2021 to January 2022. The samples were grown on Maconkey agar and blood agar medium and incubated at 37°C for 24 hours. Bacterial isolates were diagnosed through culture characteristics, microscopic examination and biochemical tests, as 25 isolates belonging to p. aeruginosa. The phenotypic detection of these isolates was conducted and it was found that 16 isolates contain efflux pumps, after which the genetic detection was done by PCR technique. The isolates were subjected to the mexAB-OprM efflux pump detection using PCR technique, and after the replication product was migrated in agarose gel, it was observed that 93.75 %) of the isolates possess the mexA gene encoding the above efflux pump, and (43.75%) the oprM gene encoding the above efflux pump also.