Molecular Detection of Enterococcal Surface Protein (esp) Gene in Enterococcus faecalis Isolated from Dental Calculus of Patients in Sari, Iran

Akhondnezhad, Mona; Bakhti, Mehrnaz; Nasrolahei, Mohtaram; Shabankhani, Bizhan; Goli, Hamid Reza

doi:10.29252/rmm.5.3.21

Cited by 3 publications

(2 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Philadelphia, USA, 53.2% of E. faecalis isolates from subgingival were resistant to tetracycline and 100% sensitive to vancomycin [10]. In another study on E. faecalis from dental calculus in Iran, the resistance rate to vancomycin, tetracycline, and ciprofloxacin was estimated as 4.6%, 11.6%, and 6.9%, respectively [26]. The differences in results of our study and previous studies are probably due to geographical areas.…”

Section: Discussioncontrasting

confidence: 72%

Distribution and Antibiotic Resistance of Streptococci and Enterococci Isolated from Dental Caries and Healthy People

Al-Taie¹,

Musawi²,

Rasheed³

2022

JoMMID

View full text Add to dashboard Cite

Introduction: Antimicrobial resistance is one of the most significant challenges globally that threatens our ability to treat infectious diseases. This study investigated Streptococci and Enterococci in dental caries and healthy individuals and determined antibiotic resistance in the recovered bacteria. Methods: One hundred and twelve samples were collected, 56 from patients with dental caries and 56 samples from the teeth and saliva of healthy people. The samples were cultured on blood agar and purified on Mitis Salivarius agar. All isolates were identified by biochemical tests and Vitek 2 system and then examined for antibiotic susceptibility by disc diffusion method. Results: Streptococci and enterococci were the most isolated agents from dental caries and the teeth and saliva of healthy people. Streptococcus spp. comprised 48.61% of bacteria in dental caries and 28.40% in healthy individuals, while Enteroccus spp. was 22.22% in dental caries and 30.84% in healthy individuals. Streptococcus mutans and Streptococcus salivarius were more prevalent in dental caries and healthy individuals, respectively, while Enterococcus faecium was detected in both dental caries and healthy individuals. Also, there were significant differences between the number of streptococci isolated from healthy and caries people (P<0.05). In isolates recovered from healthy people, the streptococcal spp. exhibited high resistance to azithromycin (82.6%), cefixime and tetracycline (91.3%), and amoxicillin (60.8%). In contrast, enterococci were resistant to tetracycline (92%) and cefixime (76%). In dental caries isolates, the streptococcal and enterococcal spp. showed the highest resistance to amoxicillin (> 68%). Conclusions: The streptococcal and enterococcal spp. comprised the most isolated bacteria from healthy individuals and dental caries and exhibited multidrug resistance.

show abstract

Section: Discussioncontrasting

confidence: 72%

Distribution and Antibiotic Resistance of Streptococci and Enterococci Isolated from Dental Caries and Healthy People

Al-Taie¹,

Musawi²,

Rasheed³

2022

JoMMID

View full text Add to dashboard Cite

show abstract

“…Antibiotic susceptibility profile of the isolates was identified by the disc agar diffusion method as recommended by the Clinical and Laboratory Standards Institute 21 on Mueller Hinton agar (Merck, Germany). The susceptibility of the enterococcal isolates was tested against ampicillin, vancomycin, tetracycline, ciprofloxacin, and erythromycin (MAST Co, United Kingdom), which has been reported in our previous study 22 along with linezolid, chloramphenicol, penicillin G, teicoplanin, and amoxicillin-clavulanic acid in the present study. Enterococcus faecalis ATCC 29212 was used as a quality control strain in the antimicrobial susceptibility testing.…”

Section: Methodsmentioning

confidence: 99%

Antibiotic Resistance and Virulence Genes in Enterococcus faecalis Isolated From Human Dental Plaque

Bakhti

Akhondnezhad

Gholami

et al. 2021

Infect Dis Clin Pract

Self Cite

View full text Add to dashboard Cite

Background: Enterococcus faecalis causing serious infections have many virulence factors to be involved in the pathogenesis. Its pathogenicity ranges from life-threatening diseases in compromised patients to less severe features, such as infection of acquired root canals with chronic periodontitis. The aim of the present study was to determine the antibiotic resistance patterns and virulence genes in E. faecalis isolated from human dental plaque. Methods:The duration of this study was from October 2017 to March 2018. The samples were collected from dental plaques of 207 Iranian patients according to a standard protocol. Enterococcus faecalis strains were identified and confirmed by microbiological and biochemical tests. Antimicrobial susceptibility was ascertained by disk agar diffusion, using the recommended protocol of the Clinical and Laboratory Standards Institute. Finally, polymerase chain reaction for the detection of E. faecalis virulence genes (efaA, ahrC, and eep) was performed with specific primers.Results: A total of 43 (76.7%) E. faecalis were identified of which 5 (11.6%), 4 (9.3%), and 1 (2.3%) isolates were resistant against linezolid, chloramphenicol, and teicoplanin, respectively, whereas all isolates were susceptible to penicillin G and amoxicillin-clavulanic acid. Most of the E. faecalis isolates were contained efaA (65.1%), and the frequencies of eep and ahrC were 44.1% and 34.8%, respectively.Conclusions: It was concluded that E. faecalis isolated from dental plaques might represent a reservoir of biofilm encoding genes. Further epidemiological studies are necessary to investigate the status of virulence factors in enterococci isolated from dental plaques.

show abstract

Molecular investigation of enterococcal surface protein (esp) gene of Enterococcus faecalis isolated from endodontic patients

Mustafa,

Al-Rawi

2023

Medicinski časopis

View full text Add to dashboard Cite

Objective. Enterococci are generally considered transient components of oral bacteria that may be a reason for several oral and systemic infections, particularly those related to dental root canal infections. The current study aims to examine the occurrence of Enterococcus surface protein, esp in Enterococcus faecalis, which is isolated from infected root canals. Methods. Forty samples were collected from endodontic patients who attended the Conservative Treatment Department in the College of Dentistry/Mosul University/Dental Teaching Hospital. Materials and Methods: All samples were traditionally examined using HiCrom TM Enterococcus faecium Agar base medium and biochemical tests. 16srRNA sequencing was performed using the polymerase chain reaction technique to confirm their identity. Then, all Enterococcus faecalis isolates were examined for the existence of esp gene coding for enterococcal surface protein using PCR assay. Results. From 40 clinical samples obtained, 31 isolates were recognized as E. faecalis by traditional methods; unexpectedly, other non-enterococci genera were also grown on HiCromTM Enterococcus faecium Agar base medium. The PCR products for the sequence-specific primers obtained from the full-length of 16S rRNA gene sequence, which belongs to E. faecalis, and the PCR products for specific primer of esp genes created bands at the position of 138bp and 932 bp on the agarose gel, respectively. The gene correlating with the aggregation of this bacteria on the canal walls was detected in a high proportion (91%) of the isolates. Conclusions. PCR assay provides an accurate, rapid, and more sensitive detection of E. faecalis. A positive correlation between esp gene and enterococcal infections in root canals has been found.

show abstract

Molecular Detection of Enterococcal Surface Protein (esp) Gene in Enterococcus faecalis Isolated from Dental Calculus of Patients in Sari, Iran

Cited by 3 publications

References 16 publications

Distribution and Antibiotic Resistance of Streptococci and Enterococci Isolated from Dental Caries and Healthy People

Distribution and Antibiotic Resistance of Streptococci and Enterococci Isolated from Dental Caries and Healthy People

Antibiotic Resistance and Virulence Genes in Enterococcus faecalis Isolated From Human Dental Plaque

Molecular investigation of enterococcal surface protein (esp) gene of Enterococcus faecalis isolated from endodontic patients

Contact Info

Product

Resources

About