Molecular Detection of Circulating Tumor Cells in the Peripheral Blood of Patients with Colorectal Cancer Using RT‐PCR: Significance of the Prediction of Postoperative Metastasis

Wang, Jaw‐Yuan; Wu, Ching‐Shuang; Lu, Chien‐Yu; Hsieh, Jan-Sing; Wu, Deng‐Chyang; Huang, Sung–Yu; Lin, Shiu‐Ru

doi:10.1007/s00268-005-0485-z

Cited by 105 publications

(83 citation statements)

References 35 publications

(56 reference statements)

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“…There are tumor markers detectable in gastric patients that can predict early stages of metastasis; some of these markers are detectable in lymph nodes, while others are present in peripheral blood (Zieglschmid et al, 2005;Ohlsson et al, 2006). The detection rate of circulating tumor cells in peripheral blood from gastric cancer patients is in the range from 27 to 88% (Conzelmann et al, 2005;Wang et al, 2006;Lloyd et al, 2006). Although many markers have been investigated individually or in several panels, the lack of both sensitivity and specificity was shown in these experiments.…”

Section: Discussionmentioning

confidence: 78%

Absolute quantification of free tumor cells in the peripheral blood of gastric cancer patients

Bayat¹,

Mokhtari²,

Rezaei‐Tavirani³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Gastric cancer remains the third most common cancer in the world. Metastatic disease is a major cause of death in about half of the patients; therefore, early diagnosis is crucial for successful outcome. This study applied a sensitive method for the detection of circulating tumor cells using specific tumor markers for early detection. A total of 80 blood samples from 40 patients and 40 age-matched healthy controls were collected for the study. Circulating mRNA levels of two tumor markers, tumor endothelial marker 8 (TEM-8) and carcinoembryogenic antigen (CEA) were evaluated using absolute quantitative real-time PCR assay in the Stratagene Mx-3000P real-time PCR system. GAPDH was used to normalize the data. TEM-8 and CEA were detected in patients' blood more than in controls, 22/40 vs 9/40, P = 0.005, and 30/40 vs 11/40, P = 0.008, respectively. The mRNA level of these markers in patients was significantly higher in comparison to normal controls (P = 0.018, 0.01). This panel showed an overall sensitivity of 64% and specificity of 73%. Statistical analysis for demographic variants did not show any significant differences. Both markers were detected more frequently and in significantly higher levels in blood samples of patients

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Section: Discussionmentioning

confidence: 78%

Absolute quantification of free tumor cells in the peripheral blood of gastric cancer patients

Bayat¹,

Mokhtari²,

Rezaei‐Tavirani³

et al. 2014

Genet. Mol. Res.

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show abstract

“…Forty-six references were assessed in full, and a further 32 studies were excluded (Figure 1). Eight studies were excluded as they reported only on preoperative blood sampling (Bessa et al, 2001;Fujita et al, 2001;Wong et al, 2001;Zhang et al, 2005;Douard et al, 2006;Iinuma et al, 2006;Wang et al, 2006;Friederichs et al, 2007). Nine studies were excluded as they contained data from which outcomes could not be extracted, of which four were excluded as they combined positive results of molecular detection of multiple samples and their effect on prognosis (Hardingham et al, 2000;Guller et al, 2002;Bosch et al, 2003;Koyanagi et al, 2008), and five were excluded as it was impossible to extract the data for outcomes of interest (White and Griffiths, 1976;Funaki et al, 1998;Wyld et al, 1998;Conzelmann et al, 2005;Wang et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

Prognostic significance of circulating tumour cells following surgical resection of colorectal cancers: a systematic review

et al. 2010

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Background: The role of adjuvant chemotherapy after resection of colorectal cancers (CRCs) is well understood for patients with stage-I or stage-III disease. Its efficacy for those with stage-II disease remains much less clear. Many investigators have sought to identify prognostic markers that might clarify which patients have the highest risk of recurrence and would, therefore, be most likely to benefit from chemotherapy. This systematic review examines evidence for the use of peripherally sampled, circulating tumour cells (CTCs) as such a prognostic marker. Methods: A comprehensive literature search was used to identify studies reporting on the significance of CTCs in the postoperative blood of CRC patients. Results: Fourteen studies satisfied the inclusion criteria. Six of the nine studies that took blood samples 24 h or more postoperatively found detection of postoperative CTCs to be an independent predictor of cancer recurrence. Conclusion: The presence of CTCs in peripheral blood at least 24 h after resection of CRCs is an independent prognostic marker of recurrence. Further studies are needed to clarify the optimal time point for blood sampling and determine the benefit of chemotherapy in CTC-positive patients with stage-II disease.

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“…In patients with colorectal cancer, the presence of CTC in peripheral blood was repeatedly confirmed and their detection ranged from 20% for localized disease and 60% in advanced CRC [10][11][12][13][14][15][16][17][18]. The clinical relevance of CTC as an independent prognostic factor was documented for cases in patients with positive CTC at diagnosis who had poorer prognosis than patients with negative CTC [10,[12][13][14][16][17][18][19][20][21][22][23][24].…”

mentioning

confidence: 99%

“…Interestingly, some authors have performed RT-PCR even without prior pre-concentration [10,27]. Aside from just detecting their presence, the molecular profile of CTC may serve as a predictor of treatment response and survival as demonstrated in several studies [11,12,[28][29][30]. The technique known as the "liquid biopsy" has recently generated a strong interest among molecular oncologists as a viable alternative in cases where tissue sampling is not available [31].…”

mentioning

confidence: 99%

Monitoring of Circulating Tumor Cells by a Combination of Immunomagnetic Enrichment and RT-PCR in Colorectal Cancer Patients Undergoing Surgery

et al. 2016

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Background. The presence of circulating tumor cells (CTC) has been reported in patients with advanced colorectal cancer. Monitoring CTC (also known as a liquid-biopsy) has recently become the center of interest for low-invasive monitoring of cancer progression and predictive biomarkers testing. Along with high-cost technology and a complex methodology, a straightforward method based on magnetic beads enrichment followed by RT-PCR is set to allow for routine CTC analysis in colorectal cancer patients. Objectives. The main purpose of this study was to evaluate the possibility of CTC detection in routine monitoring of patients starting before and continuing after surgery. Material and Methods. The investigated group consisted of 30 patients mainly in advanced stages of colorectal cancer. In all patients, CTC detection was performed prior to surgery, in a subset of 14 patients additional sampling was done during and after surgery. In all cases, peripheral blood was processed using AdnaTest ColonCancer kit, which relies on enriching CTCs using EpCAM-functionalized magnetic beads and subsequently identifying tumorspecific CEA, EGFR and GA733-2 mRNA transcripts. Results. Out of all the tested samples, CTC were found in one patient suffering from advanced disease with lung and liver metastases. There, however, the positive finding was confirmed in 3 consecutive samples acquired before, during and shortly after palliative R2 resection. Conclusions. The presence of CTC may be used to observe post-operative disease development. Due to the overall low CTC detection, further technology development may be necessary before its universal applicability to manage colorectal cancer patients (Adv Clin Exp Med 2016, 25, 6, 1273-1279).

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Molecular Detection of Circulating Tumor Cells in the Peripheral Blood of Patients with Colorectal Cancer Using RT‐PCR: Significance of the Prediction of Postoperative Metastasis

Cited by 105 publications

References 35 publications

Absolute quantification of free tumor cells in the peripheral blood of gastric cancer patients

Absolute quantification of free tumor cells in the peripheral blood of gastric cancer patients

Prognostic significance of circulating tumour cells following surgical resection of colorectal cancers: a systematic review

Monitoring of Circulating Tumor Cells by a Combination of Immunomagnetic Enrichment and RT-PCR in Colorectal Cancer Patients Undergoing Surgery

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