Molecular detection of bat coronaviruses in three bat species in Indonesia

Dharmayanti, Ni Luh Putu Indi; Nurjanah, Diana; Nuradji, Harimurti; Maryanto, Ibnu; Exploitasia, Indra; Indriani, Risa

doi:10.4142/jvs.2021.22.e70

Cited by 2 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Japan, sarbecoviruses were detected by PCR in Rhinolophus cornutus bats [142]. In Indonesia, Bat CoVs were detected in Cynopterus brachyotis, Macroglossus minimus, and Rousettus amplexicaudatus [143]. In Pakistan, out of 70 samples collected from three species of bats, including Rousettus leschenaultia, Cynopterus sphinx, and Pteropus spp, beta coronaviruses were detected in Rousettus leschenaultia bats which were closely related to MERS-like CoVs [144].…”

Section: Asiamentioning

confidence: 99%

Global Distribution and Molecular Evolution of Bat Coronaviruses

El Sayes,

Badra,

Ali

et al. 2024

Zoonotic Diseases

View full text Add to dashboard Cite

Bat coronaviruses cause a wide range of illnesses in humans and animals. Bats are known to harbor a wide diversity of Alphacoronaviruses and Betacoronaviruses. Betacoronaviruses have been linked to Severe Acute Respiratory Syndrome (SARS), Middle East Respiratory Syndrome (MERS), and other diseases such as gastroenteritis, bronchiolitis, and pneumonia. In the last 20 years, three betacoronaviruses emerged and caused widespread outbreaks in humans, including two deadly betacoronavirus epidemics, SARS-CoV, with mortality rate of 10%, and MERS-CoV, with mortality rate of 34.7%, and SARS-CoV-2, which caused the COVID-19 pandemic, with mortality rate of 3.4%. Studies have shown that bats are the main natural reservoirs for these viruses or their ancestral viruses. Observed variations in bat coronavirus genomes indicate that these viruses may have a potential to transmit to other hosts in close contact with humans and subsequently transmit to humans. As of today, there are no reported cases of direct coronavirus transmission from bats to humans. One reason for this might be that intermediate hosts are required for the transmission of bat coronaviruses to humans. Further studies are needed to map the amino acids and genomic regions responsible for the interactions between the spike of coronavirus and its receptors.

show abstract

Section: Asiamentioning

confidence: 99%

Global Distribution and Molecular Evolution of Bat Coronaviruses

El Sayes,

Badra,

Ali

et al. 2024

Zoonotic Diseases

View full text Add to dashboard Cite

show abstract

“…In Indonesia, bats and other animals are widely traded in several live animal markets. Collectors catch bats in forests or caves and sell them to traders at animal markets and restaurant owners (7). Until now, there has been a lot of hunting for bats for human consumption.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Analysis and Mutation of Sars-Cov-2 in Bats in Karst Malang City, Indonesia

Keman,

Moch Irfan Hadi,

Dedy Suprayogi

et al. 2024

JKL

View full text Add to dashboard Cite

Introduction: A group of people in China were hospitalized with an initial diagnosis of pneumonia of unknown cause. The patients were linked to a wholesale wet seafood and animal market in Wuhan, Hubei Province, China. The disease has spread to other provinces in China, Thailand, Japan, and South Korea in less than a month. SARS-CoV-2 was found to originate from bats. Therefore, this research aims to analyze SARS-CoV-2 mutation in bats in Malang Karst, Indonesia. Methods: Other bat body parts used as research samples include the brain, liver, kidneys, intestines, pancreas, fetus, blood, lungs, and ectoparasites. The samples were taken separately and placed in a container containing 10% PBF. For further analysis, we used RNA Extraction, Real-Time PCR, Sequencing, and CoV Gisaid mutation analysis software to analyze the sequencing data. Then, EMBL software will be used to analyze the phylogenetically. Results and Discussion: There was 1 sample that showed a positive result for Covid-19, namely the intestine of the Cynoptera brachyotis species. There were differences between SARS-CoV-2 in bats in Malang Karst in Indonesia compared to SARS-CoV from 2000 to 2019. The spike protein's receptor binding domain (RBD) is the most variable part of the coronavirus genome. Conclusion: From the research results, one positive sample was obtained using Real-Time PCR, and based on mutation analysis, mutations were found in SARS-CoV-2 against the SARS-CoV virus from 2000-2019. Further research is needed, especially regarding SARS-CoV-2 as a vaccine.

show abstract

Molecular detection of bat coronaviruses in three bat species in Indonesia

Cited by 2 publications

References 26 publications

Global Distribution and Molecular Evolution of Bat Coronaviruses

Global Distribution and Molecular Evolution of Bat Coronaviruses

Phylogenetic Analysis and Mutation of Sars-Cov-2 in Bats in Karst Malang City, Indonesia

Contact Info

Product

Resources

About