2012
DOI: 10.4269/ajtmh.2012.11-0346
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Abstract: Abstract. Diagnosis of dengue virus (DENV) infection in fatal cases is challenging because of the frequent unavailability of blood or fresh tissues. For formalin-fixed, paraffin-embedded (FFPE) tissues immunohistochemistry (IHC) can be used; however, it may not be as sensitive and serotyping is not possible. The application of reverse transcriptionpolymerase chain reaction (RT-PCR) for the detection of DENV in FFPE tissues has been very limited. We evaluated FFPE autopsy tissues of 122 patients with suspected … Show more

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Cited by 41 publications
(30 citation statements)
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“…Previous studies examining variation in serotype detection by the Standard Diagnostics (24) and Panbio/Bio-Rad Platelia (12) NS1 ELISAs reported generally higher sensitivities than those presented here. Interestingly, the Bio-Rad and Standard Diagnostics NS1 antigen ELISAs had relatively low sensitivities for dengue virus serotype 2 compared to previously reported sensitivities of the Bio-Rad assay for the other serotypes (7), which is significant as serotype 2 is highly prevalent in both the Americas and Asia (1,8,10,21).…”
Section: Discussionmentioning
confidence: 69%
“…Previous studies examining variation in serotype detection by the Standard Diagnostics (24) and Panbio/Bio-Rad Platelia (12) NS1 ELISAs reported generally higher sensitivities than those presented here. Interestingly, the Bio-Rad and Standard Diagnostics NS1 antigen ELISAs had relatively low sensitivities for dengue virus serotype 2 compared to previously reported sensitivities of the Bio-Rad assay for the other serotypes (7), which is significant as serotype 2 is highly prevalent in both the Americas and Asia (1,8,10,21).…”
Section: Discussionmentioning
confidence: 69%
“…Negative controls were RNA extracted from FFPE cell culture controls or tissue specimens from persons with previously confirmed infection with the following viruses: dengue types 1–4, West Nile, yellow fever, Japanese encephalitis, St. Louis encephalitis, eastern and western equine encephalitis, chikungunya, herpes, parvovirus B19, cytomegalovirus, adenovirus, enterovirus, rubella, Powassan, and Lacrosse. We extracted RNA from FFPE tissues of all 52 case-patients (multiple FFPE tissue blocks per patient) by using an optimized extraction protocol as previously described ( 26 ) and tested the samples by newly developed Zika virus NS5 and E-gene RT-PCR and by RT-PCR for dengue and chikungunya viruses ( 27 , 28 ). RT-PCR assays were performed by using a QIAGEN OneStep RT-PCR Kit (Valencia, CA, USA) and 5 μl of RNA template, according to the manufacturer’s instructions.…”
Section: Methodsmentioning
confidence: 99%
“…Similar testing was conducted on frozen and paraffin-embedded liver and small bowel tissue biopsy specimens from the multi–visceral organ recipient. Methods of viral extraction from paraffin-embedded tissue have been described previously ( 6 ). All HAV RNA–positive samples were used to sequence the HAV VP1/P2B (viral protein 1/amino terminus of 2B ) genomic region, and phylogenetic analysis was performed by comparing these sequences with archived HAV sequences contained within the CDC HAV sequence database ( 7 ).…”
Section: Methodsmentioning
confidence: 99%