Molecular detection and phylogenetic analysis of the honeybee (Apismellifera) sacbrood virus in Turkey

Kalaycı, Gülnur; Çağırgan, Abdurrahman Anıl; Pekmez, Kemal; Özkan, Buket; Kaplan, Murat

doi:10.3906/vet-1811-5

Cited by 12 publications

(4 citation statements)

References 17 publications

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“…This range can be explained by the fact that CBPV is a segmented and variable virus. In addition, the genomic variability of honeybee viruses, such as the Sacbrood virus in Turkey has been previously described (Kalayci et al, 2019;Yildirim et al, 2020). The genetic variability of CBPV was also reported in a previous study by Cagirgan (2018).…”

Section: Resultssupporting

confidence: 55%

The phylogenetic analysis of RNA-dependent RNA polymerase gene of chronic bee paralysis virus in Turkey

ÇAGIRGAN¹,

Yıldırım

Usta

2022

J Hellenic Vet Med Soc

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Chronic bee paralysis virus (CBPV) is a major contributor to global bee colony losses. However, among the RNA viruses infecting honeybees, CBPV is still not classified. In this study, samples of asymptomatic adult honey bees were randomly collected from 45 apiaries in three provinces (Burdur, Antalya, and Isparta) in Turkey. Five of the 45 samples were determined to be positive by reverse transcription polymerase chain reaction (RT-PCR). In addition, three positive samples from Burdur, Isparta, and Antalya were confirmed by sequencing. We constructed a phylogenetic tree that was divided into five main groups based on the RNA-dependent RNA polymerase gene region. The Turkish strains TUR/BUR/CBPV1, TUR/ISPT/CBPV2, and TUR/ANT/CBPV3 and the Turkish strains from obtained previous studies formed a different cluster. The sequence homology results of a phylogenetic analysis showed that the Turkish strains shared 97-98.9% of their nucleotide identity and had 96.9-99% similarity rates with each other. The strains obtained in this study and the Turkish strains detected in previous studies were also 81-84.6% similar to European, Chinese, and Uruguay strains. This research underlines the presence of CBPV in apparently healthy Turkish bee colonies and the remarkable differences in Turkish CBPV strains. Further investigation is needed to identify the molecular characterization, complete genome sequence, and pathogenesis of Turkish CBPV strains.

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Section: Resultssupporting

confidence: 55%

The phylogenetic analysis of RNA-dependent RNA polymerase gene of chronic bee paralysis virus in Turkey

ÇAGIRGAN¹,

Yıldırım

Usta

2022

J Hellenic Vet Med Soc

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“…Numerous studies have been performed on bee viruses in Turkey in the past (14,17,18,23,35,42). In these studies, it was reported that the incidence of DWV was high in certain regions in Turkey (24,42).…”

Section: Resultsmentioning

confidence: 99%

“…Although there have been numerous colony losses throughout the world, the reasons for which are mainly unknown, one of the principal factors that leads to colony loss is viruses. For this reason, bee viruses have been the subject of many different studies conducted in Turkey (14,17,18,23,35,42).…”

mentioning

confidence: 99%

Phylogenetic analysis of deformed wing virus, black queen cell virus and acute bee paralysis viruses in Turkish honeybee colonies

Çağırgan¹,

Yıldırım²,

Usta³

2020

Medycyna Weterynaryjna

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Viruses are chiefly responsible for colony losses globally, and deformed wing virus (DWV), black queen cell virus (BQCV), and acute bee paralysis virus (ABPV) are the most common viral infections in honeybee colonies. As Turkey ranks third in the world in terms of bee colonies, honeybees are of great ecological and economic importance. Thus, the aim of this study was to conduct a phylogenetic analysis of DWV, BQCV, and ABPV, all of which were detected in the southern city of Burdur, which is located along the route that migratory bees take and is a dwelling place for them. Phylogenetic trees were constructed for DWV, BQCV, and ABPV partial nucleotide sequences of the RNA helicase region, the structural polypeptide coding region, and the capsid protein region, respectively. Burdur DWV1 and DWV14 isolates were highly conserved, with 99-100% similarity, and they also shared 93-100% similarity with isolates from Europe and Korea. The two Burdur DWVs had 98% similarity. The DWV1 genome was more similar to the other Turkish isolates than the DWV14. Burdur BQCV2 and BQCV19 isolates, which were highly conserved with 97-99% similarity, formed the same cluster with other Turkish isolates but excluding the Turkey Koycegiz isolate. Burdur ABPV isolates were highly conserved with 99% similarity. Although they showed 94-97% similarity with other European isolates, they were in the same cluster as Turkish isolates. Thus, it was found that although the DWV, BQCV, and ABPV isolates obtained as a result of the present study were highly conserved, they showed differences in relation to the DWV, BWCV, and ABPV isolates that were isolated from different geographical regions.

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“…Karapınar et al (2018) detected four viruses (ABPC, CBPV, DWV, BQCV) in Van honey bees [19]. Kalaycı et al (2019) detected SBV in honey bees from Muğla province [46]. Rüstemoglu and Sipahioglu (2019) defined 6 viruses (BQCV, DWV, SBV, CBPV, KBV, IAPV) in honey bees in Hakkari province [15].…”

Section: Discussionmentioning

confidence: 99%

Determination of Mixed Virus Infection in Honey Bees from Erzurum, Türkiye

SELVİTOPİ,

EROĞLU

2023

Türk Doğa Ve Fen Dergisi

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Honey bees are the most important pollinators of agricultural products and provide pollination. Especially the worker bees, which make up the majority of the honey bee population, produce products with high economic value such as honey, pollen, propolis, royal jelly, bee venom, and beeswax. Mass deaths are observed in honey bees grown in Erzurum province, where beekeeping activities are carried out intensively in the Eastern Anatolia region of Türkiye. Epidemic diseases seen in honey bees in Erzurum have a very negative effect on the development and progress of beekeeping activities. Most of the diseases that cause epidemics in bees and cause sudden death and colony loss are of viral origin. In this study, viral pathogens that cause death in honey bees in Erzurum province were investigated and it was determined that two honeybee viruses, the black queen cell virus (BQCV), and the deformed wing virus, caused intense epidemics. In addition, phylogenetic analyzes revealed that all BQCV isolates found in this study clustered quite far from BQCV isolates previously isolated from Turkiye, while DWV isolates clustered close to Hakkari and Lithuania isolates.

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Molecular detection and phylogenetic analysis of the honeybee (Apismellifera) sacbrood virus in Turkey

Cited by 12 publications

References 17 publications

The phylogenetic analysis of RNA-dependent RNA polymerase gene of chronic bee paralysis virus in Turkey

The phylogenetic analysis of RNA-dependent RNA polymerase gene of chronic bee paralysis virus in Turkey

Phylogenetic analysis of deformed wing virus, black queen cell virus and acute bee paralysis viruses in Turkish honeybee colonies

Determination of Mixed Virus Infection in Honey Bees from Erzurum, Türkiye

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