Molecular detection and genetic diversity of Babesia gibsoni in dogs in India

Singh, Mahima; Raina, O.K.; Sankar, M.; Rialch, Ajayta; Tigga, M. N.; Kumar, Girish; Banerjee, P. S.

doi:10.1016/j.meegid.2016.03.025

Cited by 23 publications

(15 citation statements)

References 21 publications

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“…Molecular characterization of B. gibsoni based on 18S rRNA gene sequence was effective in differentiating the various genotypes of the organism [ 13 , 29 ]. Apart from employing small subunit ribosomal RNA gene sequence, heat shock protein [ 30 ] and B. gibsoni thrombospondin-related adhesive protein [ 31 ] were also reported to be useful for phylogenetic analysis of B. gibsoni . Sequencing of the PCR product obtained with newly designed primer pairs (BAGIF and BAGIR) yielded a 488 bp sequence of 18S r RNA gene which revealed 99% similarity with several corresponding sequences of B. gibsoni available in online databases.…”

Section: Discussionmentioning

confidence: 99%

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High prevalence of small Babesia species in canines of Kerala, South India

et al. 2017

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Aim:Canine babesiosis is an important vector-borne hemoparasitic disease caused by Babesia canis vogeli and Babesia gibsoni, in India. The communication places on record the salient findings of the study directed to detect and characterize the pathogenic B. gibsoni isolates of Kerala state.Materials and Methods::A total of 150 dogs were examined for the presence of hemoparasites by light microscopy as well as by PCR targeting the 18S rRNA gene of B. gibsoni. Hematological parameters were also analysed. Phylogenetic tree was constructed based on Tamura kei model adopting ML method.Results::A sensitive and specific polymerase chain reaction assay was developed with newly designed primer pair BAGI-F/BAGI-R for the amplification of 488 bp fragment of 18S rRNA gene of B. gibsoni. Out of the 150 dogs examined, molecular evidence of B. gibsoni was recorded in 47.3% animals, while light microscopy detected the infection in 26.67% cases. The phylogenetic analyses revealed that B. gibsoni, Kerala, isolate was closest and occurred together with Bareilly isolate. Anemia and thrombocytopenia were the significant hematological alterations in chronic B. gibsoni infection.Conclusion::A high prevalence of natural infection of B. gibsoni was detected among the study population. The affected animals showed anaemia and thrombocytopenia. Phylogenetic analysis of this pathogenic isolate from south India revealed the closest similarity with Bareilly isolates.

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Section: Discussionmentioning

confidence: 99%

“…Singh et al . [ 31 ] had observed that B. gibsoni endemic in dogs in India and Bangladesh formed a distinct genetic clade.…”

Section: Discussionmentioning

confidence: 99%

High prevalence of small Babesia species in canines of Kerala, South India

et al. 2017

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“…and B. canis vogeli in this study appear to be remarkably similar to previously described species with 99–100% homology to the reference sequences from the world. B. gibsoni TRAP (BgTRAP) gene encodes TRAP, a type-Itransmembrane protein and demonstrates abundant genetic diversity in Asian isolates [ 32 ]. However, fragments of the gene generated in dogs from Jiangxi shared 99.98–99.99% homology to the sequences deposited in GenBank and formed a sub-cluster with isolates from Japan and other regions of China.…”

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confidence: 99%

First molecular detection of tick-borne pathogens in dogs from Jiangxi, China

Zheng

Liu

Moumouni

et al. 2017

The Journal of Veterinary Medical Science

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In this study, blood samples obtained from 162 dogs in Jiangxi, China, were employed in molecular screening of canine tick-borne pathogens by PCR and sequencing. Babesia spp. gene fragment was detected in 12 (7.41%) dogs. All samples were negative for Hepatozoon spp., Ehrlichia canis, Coxiella spp., Borrelia spp., Rickettsia spp. and Anaplasma platys. Species-specific PCR analysis further confirmed that 8 (4.94%) and 4 (2.47%) dogs were infected by Babesia canis vogeli and Babesia gibsoni, respectively. Based on our analyses, Babesia spp. infection in Jiangxi appeared not related to age, gender, breed, usage, activity and health status or tick infestation history of the dogs. This is the first molecular report of Babesia canis vogeli and Babesia gibsoni in dogs from Jiangxi, China.

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“…Babesia gibsoni, an obligate intraerythrocytic parasitic protozoan that can cause canine babesiosis [1][2][3], spreads widely around the world. Canine babesiosis caused by B. gibsoni may cause hyperacute, acute and more commonly chronic infection [3], with the clinical symptoms of fever, hemolytic anemia and even death [4,5].…”

Section: Introductionmentioning

confidence: 99%

Annotation and characterization of Babesia gibsoni apicoplast genome

Liu

Fan

et al. 2020

Parasites Vectors

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Background: Babesia gibsoni is an apicomplexan parasite transmitted by ticks, which can infect canine species and cause babesiosis. The apicoplast is an organelle associated with isoprenoids metabolism, is widely present in apicomplexan parasites, except for Cryptosporidium. Available data indicate that the apicoplast is essential for the survival of apicomplexan parasites. Methods: Here, the apicoplast genome of B. gibsoni was investigated by high-throughput genome sequencing, bioinformatics analysis, and conventional PCR. Results: The apicoplast genome of B. gibsoni-Wuhan strain (B. gibsoni-WH) consists of a 28.4 kb circular molecule, with A + T content of 86.33%, similar to that of B. microti. Specifically, this genome encodes genes involved in maintenance of the apicoplast DNA, transcription, translation and maturation of organellar proteins, which contains 2 subunits of ribosomal RNAs, 17 ribosomal proteins, 1 EF-Tu elongation factor (tufA), 5 DNA-dependent RNA polymerase beta subunits, 2 Clp protease chaperones, 23 tRNA genes and 5 unknown open reading frames (hypothetical proteins). Phylogenetic analysis revealed high similarity of B. gibsoni apicoplast genome to that of B. orientalis and B. bovis. Conclusions: To our knowledge, this is the first report of annotation and characterization of B. gibsoni-WH apicoplast genome. The results will facilitate the development of new anti-Babesia drug targets.

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Molecular detection and genetic diversity of Babesia gibsoni in dogs in India

Cited by 23 publications

References 21 publications

High prevalence of small Babesia species in canines of Kerala, South India

High prevalence of small Babesia species in canines of Kerala, South India

First molecular detection of tick-borne pathogens in dogs from Jiangxi, China

Annotation and characterization of Babesia gibsoni apicoplast genome

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