Molecular detection and characterization of Anaplasma spp. in sheep and cattle from Xinjiang, northwest China

Yang, Jifei; Li, Youquan; Liu, Zhijie; Liu, Junlong; Niu, Qingli; Ren, Qiaoyun; Chen, Ze; Guan, Guiquan; Luo, Jianxun; Yin, Hong

doi:10.1186/s13071-015-0727-3

Cited by 92 publications

(65 citation statements)

References 31 publications

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“…In Hebei province, A. phagocytophilum was detected in H. longicornis and D. nuttalli , where H. longicornis is the predominant tick species for the transmission of this pathogen (Yaxue et al, 2011). Phylogenetically, A. phagocytophilum detected in the study was more likely to infect ruminants (Yang et al, 2015a). …”

Section: Discussionmentioning

confidence: 93%

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

et al. 2016

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Tick-borne diseases are considered as emerging infectious diseases in humans and animals in China. In this study, Ixodes persulcatus (n = 1699), Haemaphysalis concinna (n = 412), Haemaphysalis longicornis (n = 390), Dermacentor nuttalli (n = 253), and Dermacentor silvarum (n = 204) ticks were collected by flagging from northeastern China, and detected for infection with Anaplasma, Ehrlichia, Babesia, and Hepatozoon spp. by using nested polymerase chain reaction assays and sequencing analysis. Anaplasma phagocytophilum was detected in all tick species, i.e., I. persulcatus (9.4%), H. longicornis (1.9%), H. concinna (6.5%), D. nuttalli (1.7%), and D. silvarum (2.3%); Anaplasma bovis was detected in H. longicornis (0.3%) and H. concinna (0.2%); Ehrlichia muris was detected in I. persulcatus (2.5%) and H. concinna (0.2%); Candidatus Neoehrlichia mikurensis was only detected in I. persulcatus (0.4%). The Ehrlichia variant (GenBank access number KU921424), closely related to Ehrlichia ewingii, was found in H. longicornis (0.8%) and H. concinna (0.2%). I. persulcatus was infected with Babesia venatorum (1.2%), Babesia microti (0.6%), and Babesia divergens (0.6%). Additionally, four Babesia sequence variants (GenBank access numbers 862303–862306) were detected in I. persulcatus, H. longicornis, and H. concinna, which belonged to the clusters formed by the parasites of dogs, sheep, and cattle (B. gibsoni, B. motasi, and B. crassa). Two Hepatozoon spp. (GenBank access numbers KX016028 and KX016029) associated with hepatozoonosis in Japanese martens were found in the collected ticks (0.1–3.1%). These findings showed the genetic variability of Anaplasma, Ehrlichia, Babesia, and Hepatozoon spp. circulating in ticks in northeastern China, highlighting the necessity for further research of these tick-associated pathogens and their role in human and animal diseases.

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Section: Discussionmentioning

confidence: 93%

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

et al. 2016

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“…Furthermore, among all the risk factors associated, tick presence and unhygienic condition were highly associated with infection as shown by coefficient of correlation (Table 2). Indirect ELISA Real-time PCR [2,8,[16][17][18]. In small ruminants, the overall prevalence rate was 33.87%…”

Section: Resultsmentioning

confidence: 99%

“…It is an important issue for animal breeders because of the economic losses associated with it and its threat to human health [7][8][9]. Pakistan is an agricultural country and livestock is an important sector with 11.9% contribution in national GDP and 56.2% share in agricultural economy [10].…”

Section: Introductionmentioning

confidence: 99%

First detection on prevalence of Anaplasma marginale in sheep and goat in Karak District, Pakistan

Hussain¹,

Junaid²,

Gul³

et al. 2017

APJTD

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“…Previous studies on A. phagocytophilum infection in sheep and goats have been conducted in a forest area of northeastern China [22], four counties in southeastern China [7] and six counties in Xinjiang, northwest China [20]. The results of the present study in the six provinces of China showed that the overall infection rate of A. phagocytophilum was 3.6%, which was much lower than that found in livestock from Changbai Mountain in Jilin Province, where there was a prevalence of 6.7% [22], as well as in sheep and goats in four counties in southeastern China, where there was a prevalence of 6.1% [7].…”

Section: Discussionmentioning

confidence: 99%

“…A. phagocytophilum has been detected in rodents, sheep, rabbits, cattle, goats and ticks in different areas of China [720212223]. In addition, the presence of A. bovis and A. ovis in sheep and goats, as well as A. marginale in cattle has been reported in China [72025]. In a previous study, 262 field blood samples of goats collected from four cities in China were analyzed for the presence of A. phagocytophilum , A. bovis and A. ovis [7].…”

Section: Introductionmentioning

confidence: 99%

Molecular and phylogenetic analysis ofAnaplasmaspp. in sheep and goats from six provinces of China

Zhang

et al. 2016

J Vet Sci

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Members of the genus Anaplasma are important emerging tick-borne pathogens in both humans and animals in tropical and subtropical areas. Here, we investigated the presence of Anaplasma spp. in 621 sheep and 710 goats from six provinces of China. Polymerase chain reaction (PCR) and DNA sequencing were conducted to determine the prevalence of Anaplasma (A.) phagocytophilum, A. ovis and A. bovis targeting the 16S ribosomal RNA or the major surface protein 4 gene. PCR revealed Anaplasma in 39.0% (240/621) of sheep and 45.5% (323/710) of goats. The most frequently detected species was A. ovis (88/621, 14.2% for sheep; 129/710, 18.2% for goats), followed by A. bovis (60/621, 9.7% for sheep; 74/710, 10.4% for goats) and A. phagocytophilum (33/621, 5.3% for sheep; 15/710, 2.1% for goats). Additionally, eight sheep and 20 goats were found to be infected with three pathogens simultaneously. DNA sequencing confirmed the presence of these three Anaplasma species in the investigated areas, and phylogenetic analysis indicated that there was geographic segregation to a certain extent, as well as a relationship between the host and cluster of A. ovis. The results of the present study provide valuable data that helps understand the epidemiology of anaplasmosis in ruminants from China.

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Molecular detection and characterization of Anaplasma spp. in sheep and cattle from Xinjiang, northwest China

Cited by 92 publications

References 31 publications

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

First detection on prevalence of Anaplasma marginale in sheep and goat in Karak District, Pakistan

Molecular and phylogenetic analysis ofAnaplasmaspp. in sheep and goats from six provinces of China

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