Molecular cytogenetic characterization of a novel wheat–Psathyrostachys huashanica Keng 5Ns (5D) disomic substitution line with stripe rust resistance

Li, Jiachuang; Yao, Xiaoni; Yang, Zujun; Cheng, Xueni; Yuan, Fengping; Liu, Yang; Wu, Jun; Yang, Qin; Zhao, Jixin; Chen, Xinhong

doi:10.1007/s11032-019-1014-3

Cited by 15 publications

(19 citation statements)

References 59 publications

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“…A wheat-L. mollis 2Ns, 3Ns (2D, 3D) double substitution line and a wheat-P. huashanica 5Ns (5D) substitution line were identified by Li J. C. et al (2019) and Zhao et al (2019) using SSR, EST-STS, and SCAR markers. SSR markers located at a specific position of wheat chromosome could be regarded as tags for the presence of chromosomes (arms) (Röder et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Molecular Cytogenetic and Agronomic Characterization of the Similarities and Differences Between Wheat–Leymus mollis Trin. and Wheat–Psathyrostachys huashanica Keng 3Ns (3D) Substitution Lines

Cheng

et al. 2021

Front. Plant Sci.

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Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs) and Leymus mollis Trin. (2n = 4x = 28, NsNsXmXm) are valuable resources for wheat breeding improvement as they share the Ns genome, which contains diverse resistance genes. To explore the behaviors and traits of Ns chromosomes from the two species in wheat background, a series of wheat–P. huashanica and wheat–L. mollis substitution lines were developed. In the present study, line DH109 (F7 progeny of wheat–P. huashanica heptaploid line H8911 × durum wheat Trs-372) and line DM131 (F8 progeny of wheat–L. mollis octoploid line M842 × durum wheat Trs-372) were selected. Cytological observation combined with genomic in situ hybridization experiments showed that DH109 and DM131 each had 20 pairs of wheat chromosomes plus a pair of alien chromosomes (Ns chromosome), and the pair of alien chromosomes showed stable inheritance. Multiple molecular markers and wheat 55K SNP array demonstrated that a pair of wheat 3D chromosome in DH109 and in DM131 was substituted by a pair of P. huashanica 3Ns chromosome and a pair of L. mollis 3Ns chromosome, respectively. Fluorescence in situ hybridization (FISH) analysis confirmed that wheat 3D chromosomes were absent from DH109 and DM131, and chromosomal FISH karyotypes of wheat 3D, P. huashanica 3Ns, and L. mollis 3Ns were different. Moreover, the two lines had many differences in agronomic traits. Comparing with their wheat parents, DH109 expressed superior resistance to powdery mildew and fusarium head blight, whereas DM131 had powdery mildew resistance, longer spike, and more tiller number. Therefore, Ns genome from P. huashanica and L. mollis might have some different effects. The two novel wheat–alien substitution lines provide new ideas and resources for disease resistance and high-yield breeding on further utilization of 3Ns chromosomes of P. huashanica or L. mollis.

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Section: Discussionmentioning

confidence: 99%

Molecular Cytogenetic and Agronomic Characterization of the Similarities and Differences Between Wheat–Leymus mollis Trin. and Wheat–Psathyrostachys huashanica Keng 3Ns (3D) Substitution Lines

Cheng

et al. 2021

Front. Plant Sci.

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“…Crosses between wheat and P. huashanica are relatively recent, and P. huashanica is significant among numerous species related to wheat because of its excellent agronomic characteristics. Several wheat-P. huashanica progeny lines were generated in previous studies, including disomic addition lines [33], disomic substitution lines [34], and small fragment translocation lines [17]. In our laboratory, 35 derived lines were developed by crossing common wheat 7182 with P. huashanica via embryo rescue culture and backcrossing, and these intermediate materials with different agronomic traits provide a suitable foundation for exploiting genes from P. huashanica.…”

Section: Discussionmentioning

confidence: 99%

A 1Ns Disomic Addition from Psathyrostachys Huashanica Keng Confers Resistance to Powdery Mildew in Wheat

Liu¹,

Hou²,

Li³

et al. 2020

Agronomy

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Powdery mildew is a fungal disease that threatens wheat production throughout the world. Breeding resistant cultivars is an effective way to reduce harm caused by powdery mildew. In this study, 35 wheat-Psathyrostachys huashanica-derived lines were developed by crossing common wheat and P. huashanica Keng (2n = 2x = 14, NsNs) using embryo culture. Resistance to powdery mildew in the derived lines was identified at the seedling and adult stages. Line H5-5-4-2 was selected with immunity to powdery mildew at both growth stages. The chromosome structure of this line was characterized by cytology, genomic in situ hybridization (GISH), and expressed sequence tag-sequence-tagged site (EST-STS) analysis. The chromosome configuration was 2n = 44 = 22II. Two P. huashanica chromosomes with strong hybridization signals were detected by GISH analysis. Among 83 EST-STS markers that covered all seven homologous groups in wheat, three pairs of STS markers, BE497584, BF202643, and BG262410, located in wheat homologous group 1 amplified clear specific bands related to P. huashanica. The results indicated that resistant line H5-5-4-2 was a wheat-P. huashanica 1Ns disomic addition line.

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“…Genome specific DNA markers can be used to identify specific wheat chromosomes and determine homoeology of alien chromosome(s) with wheat. Du et al [13] identified a wheat-P. huashanica 5Ns disomic addition line using EST-STS markers; Li et al [19] identified a wheat-P. huashanica 5Ns(5D) disomic substitution line using SSR, EST-STS, sequence characterized amplified region (SCAR) markers. In this study, we used wheat genomespecific SSRs and P. huashanica genome specific EST-STSs to determine the chromosomal composition of TR77 and found that SSRs on 5DS and 5DL near the centromere amplified wheat genome-specific bands in parent 7182, but not in the translocation line TR77, whereas three EST-STSs on the fifth homoeologous group [13] amplified Ns genome-specific bands in both TR77 and P. huashanica.…”

Section: Discussionmentioning

confidence: 99%

“…The colchicine treated H811 was crossed and backcrossed to 7182 to get heptaploid hybrid H8911 (2n = 49, AABBDDNs). Subsequently, H8911 was then backcrossed to 7182 or crossed to several other wheat cultivars, and generated a series of wheat-P. huashanica derived lines including wheat-P. huashanica 1Ns-7Ns disomic addition lines [12][13][14][15][16][17], 2Ns(2D) disomic substitution line [18], 5Ns(5D) disomic substitution line [19], and translocation lines [20,21]. These wheat-P. huashanica derived lines have more desired agronomic traits than their wheat parents, demonstrating that P. huashanica is a useful wild relative for wheat improvement.…”

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confidence: 99%

Molecular cytogenetic characterization of a novel wheat–Psathyrostachys huashanica Keng T3DS-5NsL•5NsS and T5DL-3DS•3DL dual translocation line with powdery mildew resistance

Zhao

Cheng

et al. 2020

BMC Plant Biol

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Background: Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs) carries many outstanding agronomic traits, therefore is a valuable resource for wheat genetic improvement. Wheat-P. huashanica translocation lines are important intermediate materials for wheat breeding and studying the functions of alien chromosomes. However, powdery mildew resistance in these translocation lines has not been reported previously.Results: This study developed a novel wheat-P. huashanica translocation line TR77 by selecting a F 7 progeny from the cross between heptaploid hybrid H8911 (2n = 7x = 49, AABBDDNs) and durum wheat line Trs-372. Chromosome karyotype of 2n = 42 = 21II was observed in both mitotic and meiotic stages of TR77. Genomic in situ hybridization analysis identified two translocated chromosomes that paired normally at meiosis stage in TR77. Molecular marker analysis showed that part of chromosome 5D was replaced by part of alien chromosome fragment 5Ns. It meant replacement made part 5DL and part 5NsL·5NsS existed in wheat background, and then translocation happened between these chromosomes and wheat 3D chromosome. Fluorescence in situ hybridization demonstrated that TR77 carries dual translocations: T3DS-5NsL·5NsS and T5DL-3DS·3DL. Analysis using a 15 K-wheat-SNP chip confirmed that SNP genotypes on the 5D chromosome of TR77 matched well with these of P. huashanica, but poorly with common wheat line 7182. The translocation was physically located between 202.3 and 213.1 Mb in 5D. TR77 showed longer spikes, more kernels per spike, and much better powdery mildew resistance than its wheat parents: common wheat line 7182 and durum wheat line Trs-372.(Continued on next page)

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Molecular cytogenetic characterization of a novel wheat–Psathyrostachys huashanica Keng 5Ns (5D) disomic substitution line with stripe rust resistance

Cited by 15 publications

References 59 publications

Molecular Cytogenetic and Agronomic Characterization of the Similarities and Differences Between Wheat–Leymus mollis Trin. and Wheat–Psathyrostachys huashanica Keng 3Ns (3D) Substitution Lines

Molecular Cytogenetic and Agronomic Characterization of the Similarities and Differences Between Wheat–Leymus mollis Trin. and Wheat–Psathyrostachys huashanica Keng 3Ns (3D) Substitution Lines

A 1Ns Disomic Addition from Psathyrostachys Huashanica Keng Confers Resistance to Powdery Mildew in Wheat

Molecular cytogenetic characterization of a novel wheat–Psathyrostachys huashanica Keng T3DS-5NsL•5NsS and T5DL-3DS•3DL dual translocation line with powdery mildew resistance

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