Molecular Cloning, Tissue Distribution, and Chromosomal Localization of the Human Homolog of the R2/Th/Stylar Ribonuclease Gene Family

Cloning and characterization of a senescence inducing and class II tumor suppressor gene in ovarian carcinoma at chromosome region 6q27

“…The gene was cloned at 6q27 Acquati et al, 2000), a chromosome region often deleted in ovarian tumors and in di erent human neoplasms, such as melanoma, breast cancer, nonHodgkin B-cell lymphoma and several others (Saito et al, 1993;Foulkes et al, 1993;Cooke et al, 1996;Orphanos et al, 1995;Tibiletti et al, 1996;Develee et al, 1991;Theile et al, 1996;Chappel et al, 1997;Queimado et al, 1995;DeSouza et al, 1995;Honchel et al, 1996;Morita et al, 1991;Tahara et al, 1996;Millikin et al, 1991;Gaidano et al, 1992;Hayashi et al, 1990). RNASE6PL belongs to a group of RNases which are very conserved among species Anderson et al, 1986;Clark et al, 1990;Schneider et al, 1993;Meadoe and Kennell, 1990;McClure et al, 1989;Hime et al, 1995).…”

“…Physical measurements indicate that the distance between D6S193 and D6S149 is approximately 900 Kb, whereas the smaller D6S193 ± D6S297 interval spans about 100 Kb (Tibiletti et al, 1998;Hauptschein et al, 1998). Within the latter genomic region we have recently mapped, cloned and characterized a cDNA (RNASE6PL) Acquati et al, 2000) coding for a protein with a great similarity to a class of highly conserved cytoplasmic RNases present in plants (Anderson et al, 1986;Clark et al, 1990), animal viruses (Schneider et al, 1993), bacteria (Meador and Kennell, 1990), fungi (McClure et al, 1989), drosophila (Hime et al, 1995) and mouse . Interestingly, although these enzymes from di erent organisms ful®ll di erent biological functions, a common feature of their activity is the control of cell growth (McClure et al, 1990).…”

The RNase Rny1p cleaves tRNAs and promotes cell death during oxidative stress in Saccharomyces cerevisiae

“…The similarity of tRNA cleavage in yeast and mammalian cells, and the ability of the human orthologue RNASET2 to complement an rny1∆ yeast strain, suggested that the biological response to oxidative stress involving these nucleases is a con served process. Previous work has shown that additional ex pression of RNASET2, or addition of recombinant RNASET2 protein, inhibits colony formation and metastasis of tumor cell lines, though in a manner independent of the catalytic activity of RNASET2 (Acquati et al, 2001(Acquati et al, , 2005Smirnoff et al, 2006). This suggested two possible overlapping mechanisms by which Rny1p could influence cell fate.…”

Stress-induced RNASET2 overexpression mediates melanocyte apoptosis via the TRAF2 pathway in vitro

“…Studies to determine the role of RNASET2 in cancers have elucidated its suppressive effects – independent of its nuclease activity – on cell growth and metastatic potential, both in vitro and in vivo . 5, 6, 7 To date, RNASET2 is the only member of the Rh/T2/S family of acidic hydrolases identified in humans, 8 but more recent studies of the RNASET2 ortholog in Saccharomyces cerevisiae , Rny1, have demonstrated a similar function, in that Rny1 can mediate inhibition of cell growth in response to oxidative stress conditions and that this activity is independent of the enzyme's catalytic activity. 9 …”