Molecular cloning of glutathione reductase cDNAs and analysis of GR gene expression in cowpea and common bean leaves during recovery from moderate drought stress

Torres-Franklin, Maria Lucia; Contour-Ansel, Dominique; Zuily-Fodil, Yasmine; Pham-Thi, Anh-Thu

doi:10.1016/j.jplph.2007.03.011

Cited by 54 publications

(32 citation statements)

References 37 publications

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“…The expression of GR is enhanced in plants by drought/desiccation (Contour-Ansel et al 2006;Torres-Franklin et al 2008) and salt (Kaminaka et al 1998). Abscisic acid (ABA) and H 2 O 2 accumulate in plants under salt stress (Moons et al 1995;Montero et al 1997;Tsai et al 2005;Hong et al 2007).…”

Section: Introductionmentioning

confidence: 99%

NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid

et al. 2009

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Reactive oxygen species (ROS) play an important role in NaCl stress. Plants tolerant to NaCl stress may evolve certain strategies to remove these ROS, thus reducing their toxic effects. Therefore, the expression patterns of the gene family encoding glutathione reductase (GR, EC 1.6.4.2) were analyzed in roots of etiolated rice (Oryza sativa L.) seedlings in response to NaCl stress. Semi-quantitative RT-PCR was applied to quantify the mRNA levels for one cytosolic (OsGR2) and two chloroplastic (OsGR1 and OsGR3) isoforms of glutathione reductase identified in the rice genome. The expression of OsGR2 and OsGR3 but not OsGR1 was increased in rice roots treated with 150 mM NaCl. The Rab16A is an abscisic acid (ABA)-responsive rice gene. Increasing concentrations of ABA, from 1 to 12 μM, progressively increased the expression of OsRab16A in rice roots. In the present study, the ABA level was judged by the expression of OsRab16A in rice roots. Treatment with 150 mM NaCl induced the expression of OsRab16A, and the expression increased with increasing concentrations of ABA, which suggests that ABA may be involved in this response in rice roots. In fact, exogenous application of ABA enhanced the expression of OsGR2 and OsGR3 in rice roots. On inhibiting ABA accumulation with sodium tungstate (Tu), an inhibitor of ABA biosynthesis, the expression of OsGR2 and OsGR3 was still induced by NaCl; therefore, NaCl-triggered expression of OsGR2 and OsGR3 in rice roots is not mediated by accumulation of ABA. However, NaCl treatment could induce H 2 O 2 production in rice roots, and H 2 O 2 treatment resulted in enhanced OsGR2 and OsGR3 induction. On inhibiting the NaCl-induced accumulation of H 2 O 2 with diphenylene iodonium, the expression of OsGR2 and OsGR3 was also suppressed. Moreover, the increase in H 2 O 2 level was prior to the induction of OsGR2 and OsGR3 in NaCl-treated rice roots. Thus, H 2 O 2 , but not ABA, is involved in regulation of OsGR2 and OsGR3 expression in NaCltreated rice roots.

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Section: Introductionmentioning

confidence: 99%

NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid

et al. 2009

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“…This faster recovery could be attributed to the higher GR activity in transgenic potato. Similarly, up regulation of GR was associated with recovery from drought in tolerant cow pea cultivars (Torres-Franklin et al 2008). In drought tolerant wheat cultivars, expression level and early induction of GST during drought was suggested to play a major role in protection of cell optimal metabolism (Galle et al 2009).…”

Section: Discussionmentioning

confidence: 98%

Greater protection against oxidative damages imposed by various environmental stresses in transgenic potato with higher level of reduced glutathione

et al. 2010

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Potato (Solanum tuberosum L.) is the world's number one non-cereal food crop and ranks fourth among most important crops grown worldwide in terms of acreage, yield and value. In order to maintain greater protection against environmental stresses, we developed transgenic potato overexpressing Arabidopsis thaliana glutathione reductase gene (AtGR1). The transgenic potato maintained up to 6.5 folds higher GR activity, 5.8 folds glutathione (GSH) contents and up to 2.2 folds higher glutathione S-transferase activity compared to non transformed plants (NT). Interestingly, while the transgenic plants exhibited decreased dehydroascorbate reductase (DHAR) activity, the relative reduced ascorbate (AsA) contents were higher while the relative dehydroascorbate (DHA) were lower compared to NT which provide a support to the hypothesis that an active glutathione-independent pathway for DHA reduction might exists in vivo. The transgenic plants maintained an enhanced tolerance to methylviologen, and cadmium. When subjected to drought stress, the transgenic plants exhibited faster recovery with less visual injury compared to NT. These results suggest that manipulation of glutathione levels provides reliable strategy for the development of industrial transgenic potato plants with enhanced tolerance to multiple environmental stresses.

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“…The experiments proved that the tolerance of French beans to climatic stresses involved many stages where not only a single enzyme or compound is responsible for the overexpression or accumulation of antioxidants. The recovery ability after desiccation relates to the maintenance or elevation of level of antioxidants (Torres-Franklin et al 2008). The rapid recovery of plants after shorter or prolonged stress periods was affected by their protective mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…Decreases in sugars and oligosaccharides in Arabidopsis have been shown (Anderson and Kohorn 2001) while in others, such as maize and rice, sugars accumulated during drought (Pelleschi et al 1997;Vue et al 1998). The accumulation or absence of these compounds was determined by overexpression of regulated genes or down-regulated genes (Liu et al 2007;Micheletto et al 2007;Meglic et al 2008;Torres-Franklin et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Study of the defensive mechanism against drought in French bean (Phaseolus vulgaris L.) varieties

et al. 2010

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The response to drought of six green-and yellowpodded varieties of green bean was investigated in a phytotron. These varieties were also grown without irrigation in field experiments and analysis of leaf samples was carried out three times; before and under flowering and pod ripening, respectively. At first, the carotene and raffinose contents of leaves increase during mild drought (30/15°C). High temperature (35/25°C) combined with water deficiency resulted in a considerable decrease in leaf weight, chlorophyll b, maltose content, as well as the level of water-soluble antioxidants of the leaves, while the content of lipid-soluble antioxidants and raffinose content increased when compared with the control. The yellow-podded varieties of green beans responded to drought much more sensitively than the green-podded ones. The results suggested that a selection method based on the activity of antioxidants in the leaves can be used for testing the adaptability of numerous bean genotypes to drought.

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Molecular cloning of glutathione reductase cDNAs and analysis of GR gene expression in cowpea and common bean leaves during recovery from moderate drought stress

Cited by 54 publications

References 37 publications

NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid

NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid

Greater protection against oxidative damages imposed by various environmental stresses in transgenic potato with higher level of reduced glutathione

Study of the defensive mechanism against drought in French bean (Phaseolus vulgaris L.) varieties

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