Molecular cloning of chicken TET family genes and role of chicken TET1 in erythropoiesis

Okuzaki, Yuya; Kaneoka, Hidenori; Nishijima, Ken‐ichi; Murakami, Seitaro; Ozawa, Yuki; Iijima, Shinji

doi:10.1016/j.bbrc.2017.06.113

Cited by 6 publications

(6 citation statements)

References 33 publications

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“…This demethylation mechanism is similar to the passive demethylation of 5-methyl cytosine. Similar to mammals, TET family proteins are expressed in chickens and are involved in DNA demethylation ( Okuzaki et al , 2017 ). In this study, the gene expression of TET-1 in the hypothalamus increased with increasing duration of thermal conditioning ( Fig.…”

Section: Discussionmentioning

confidence: 99%

mRNA Expressions of Methylation Related Enzymes and Duration of Thermal Conditioning in Chicks

2022

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DNA methylation regulates gene expression by modifying the nucleosome structure of DNA, without altering the gene sequence. It has been reported that DNA methylation reactions are catalyzed by several enzymes. In chickens, thermalconditioning treatment affects the central DNA methylation levels. The purpose of this study was to clarify the changes in DNA methylation and demethylation factors during thermal conditioning in the hypothalamus of 3-day-old chicks. Male chicks (3-days old) were exposed to 40 ± 0.5 C as a thermal conditioning treatment for 1, 2, 6, 9, or 12 h.The control chicks were kept in a thermoneutral zone (30 ± 0.2 °C). After thermal conditioning, the mRNA levels of DNA methyltransferase (DNMT)-1, -3a, -3b, and teneleven translocation (TET)-1, -2, and -3 in the hypothalamus were measured by q-PCR.The mRNA levels of DNMT-3a and TET-1 were increased by thermal conditioning.Moreover, the expression level of TET-1 increased with the loading time of the thermal conditioning. The gene expressions of DNMT-1, DNMT-3b, TET-2, and TET-3 were not affected by thermal conditioning. Since DNMT-3a is a catalyst for de-novo DNA methylation and TET-1 catalyzes the oxidation of methylated cytosine, it is suggested that the thermal conditioning increased the activation of DNA methylation and demethylation factors, which occur in the hypothalamus of neonatal chicks.

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Section: Discussionmentioning

confidence: 99%

mRNA Expressions of Methylation Related Enzymes and Duration of Thermal Conditioning in Chicks

2022

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“…DNMTs expression level increased and the catalysis of DNMTs contributes to its hypermethylation in tumorous spleens. TETs may be involved in the demethylation of 5-methylcytosine through 5-hydroxymethylcytosine as an intermediate in the chicken [ 44 – 46 ]. The expression of TET2 was downregulated in tumorous spleens, which may contribute to the lower demethylation in the upstream region of gga-miR-130b-3p gene.…”

Section: Discussionmentioning

confidence: 99%

“…Primers for DNMT1, DNMT3a and DNMT3b genes used for the RT-qPCR were reffered from Tian et al (2013) [ 23 ]. Primers for TET1, TET2 and TET3 genes used for the RT-qPCR were reffered from Okuzaki et al (2017) [ 46 ].The relative genes expression level was calculated with reference to expression of 5S and β-actin. The results are described as the fold change determined by 2 −ΔΔCt method.…”

Section: Methodsmentioning

confidence: 99%

Gga-miR-130b-3p inhibits MSB1 cell proliferation, migration, invasion, and its downregulation in MD tumor is attributed to hypermethylation

Zhao

Han

et al. 2018

Oncotarget

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Marek's disease is an oncogenic and lymphoproliferative disease of chickens caused by Marek's disease virus. Hypermethylation or hypomethylation of CpG islands in gene promoter region are involved in the initiation and progression of carcinogenesis. In this study, we analyzed differential methylation levels of upstream region of gga-miR-130b-3p gene between Marek's disease virus-infected tumorous and non-infected spleens. Around the upstream 1 kb of gga-miR-130b-3p gene, two amplicons were designed that covered 616 bp. There were forty-eight CpG sites in this region. CpG sites in this region presented higher methylation level in tumorous spleens compared with that in non-infected ones. There were eight CpG sites significantly hypermethylated in tumorous spleens. The expression level of three DNA methyltransferases including DNMT1, DNMT3a and DNMT3b increased and the expression level of Tet ten-eleven translocation protein 2 remarkably decreased in tumorous spleens. Hypermethylation in the upstream region of gga-miR-130b-3p gene might be a direct reason for its downregulation in MD tumorous tissues. Moreover, cell proliferation of Marek's disease lymphoblastoid cell line MDCC-MSB1 was remarkably inhibited at 24, 36, 48, 60 and 72 h post-gga-miR-130b-3p-agomir transfection. The transwell migration assay indicated cell number of migration was significantly lower in miRNA agomir transfection group. Matrix metalloproteinases MMP2 and MMP9 are involved in tumor invasion, and their protein levels were significantly downregulated at 72 h post-miRNA-agomir transfection. Collectively, these results indicated that hypermethylation in upstream region of gga-miR-130b-3p gene contributed to its downregulation in tumorous tissues. Gga-miR-130b-3p plays an inhibitory role in lymphomatous cell transformation.

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“…Chickens express about the same number of TET enzymes as mammals (TET1/2/3) but very little is known about their function in the chicken genome (Okuzaki et al 2017). In a recent study, the chicken TET genes (cTET1/2/3) were cloned, and their expression levels determined at different developmental stages in the chick embryo and in adult chickens.…”

Section: Non-coding Rnamentioning

confidence: 99%

The chicken model organism for epigenomic research

Beacon

Davie

2021

Genome

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The chicken model organism has advanced the areas of developmental biology, virology, immunology, oncology, epigenetic regulation of gene expression, conservation biology, and genomics of domestication. Further, the chicken model organism has aided in our understanding of human disease. Through the recent advances in high-throughput sequencing and bioinformatic tools, researchers have successfully identified sequences in the chicken genome that have human orthologs, improving mammalian genome annotation. In this review, we highlight the importance of chicken as an animal model in basic and pre-clinical research. We will present the importance of chicken in poultry epigenetics and in genomic studies that trace back to their ancestor, the last link between human and chicken tree of life. There are still many genes of unknown function in the chicken genome yet to be characterized. By taking advantage of recent sequencing technologies, it is possible to gain further insight into the chicken epigenome.

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Molecular cloning of chicken TET family genes and role of chicken TET1 in erythropoiesis

Cited by 6 publications

References 33 publications

mRNA Expressions of Methylation Related Enzymes and Duration of Thermal Conditioning in Chicks

mRNA Expressions of Methylation Related Enzymes and Duration of Thermal Conditioning in Chicks

Gga-miR-130b-3p inhibits MSB1 cell proliferation, migration, invasion, and its downregulation in MD tumor is attributed to hypermethylation

The chicken model organism for epigenomic research

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