“…Each ligation reaction was then transformed into JM109 competent cells according to the method of Chung et al (Chung et al, 1989) and plated on ampicillin containing plates. The transformants were screened to find recombinant DNA containing colonies by Quick Screening (Huang et al, 2004). In Quick Screening, a toothpick was used to isolate a colony and the cells were resuspended in 25 μl of STE solution (100 mM NaCl, 20 mM Tris-Cl pH 7.5, and 10 mM EDTA).…”