Molecular cloning of a teleost growth hormone receptor and its functional interaction with human growth hormone

Lee, Leo T. O.; Nong, Guangmin; Chan, Yat‐Sun; Tse, Dicky L.Y.; Chen, Cheng

doi:10.1016/s0378-1119(01)00488-7

Cited by 82 publications

(42 citation statements)

References 27 publications

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“…The homology analysis based on the amino acid sequences revealed that the tilapia GH-R showed the highest identity (74%) to the GH-R of turbot, Scophthalmus maximus (Calduch-Giner et al 2001). The tilapia GH-R also shared identities with the counterparts of seabream, Acanthopagrus schlegel (Tse et al 2003) (73%), goldfish, Carassius auratus (Lee et al 2001) (49%), masu salmon, Oncorhynchus masou (AB071216) (40%), mouse, Mus musculus (AF120489) (34%), and human, Homo sapiens (NM-000163) (35%). On the other hand, the tilapia GH-R shared only 21% identity with tilapia PRL receptor (PRL-R) (L34783).…”

Section: Cdna Cloning Of Tilapia Gh-rmentioning

confidence: 99%

“…On the other hand, localization of IGF-I and IGF-I receptor were reported in the ovarian follicle of tilapia and seabream, suggesting that fish also have an IGF-I system within the ovary (Reinecke et al 1997, Perrot et al 2000. Recently, GH-R cDNA has been isolated in several teleost species (Calduch-Giner et al 2001, Lee et al 2001, Tse et al 2003. However, the mode of actions of GH/IGF-I in ovarian development in the fish remains unknown.…”

Section: Introductionmentioning

confidence: 99%

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Identification of the growth hormone receptor in an advanced teleost, the tilapia (Oreochromis mossambicus) with special reference to its distinct expression pattern in the ovary

Kajimura

Kawaguchi²,

Kaneko

et al. 2004

Journal of Endocrinology

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There is considerable evidence that the GH/IGF-I axis plays an important role in female reproduction. We report the isolation and characterization of the GH receptor (GH-R) and its gene expression profile during oogenesis in the tilapia, Oreochromis mossambicus. cDNA encoding GH-R was cloned and sequenced from the tilapia liver. The predicted GH-R preprotein consisted of 635 amino acids and contained a putative signal peptide, an extracellular region with a characteristic motif, a single transmembrane region, and a cytoplasmic region with conserved box 1 and 2 domains. The tilapia GH-R shared 34-74% identities with known GH-Rs in vertebrates. A binding assay using COS-7 cells showed that the cloned GH-R bound specifically to tilapia GH. Northern blot analysis showed a single mRNA transcript in the liver and ovary. In situ hybridization revealed intense signals of GH-R in the cytoplasm and nucleus of immature oocytes. The granulosa and theca cells surrounding vitellogenic oocytes also contained the GH-R mRNA signals. About a tenfold greater level of GH-R mRNA was found in the immature oocytes versus the mature oocytes, along with high levels of IGF-I mRNA. There were no significant changes in mRNA levels of GH-R and IGF-I in the liver or in plasma IGF-I levels during oocyte development. No correlation was found between hepatic GH-R mRNA and ovarian GH-R mRNA. These results suggest that the GH/IGF-I axis in the ovary may be involved in the early phases of oogenesis, under a different regulatory mechanism of GH-R gene expression from that of the liver.

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Section: Cdna Cloning Of Tilapia Gh-rmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of the growth hormone receptor in an advanced teleost, the tilapia (Oreochromis mossambicus) with special reference to its distinct expression pattern in the ovary

Kajimura

Kawaguchi²,

Kaneko

et al. 2004

Journal of Endocrinology

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“…T he characterist ic landm ark of GHR is t he YGEF S mot if found w it hin common carp GH R in t he position of aa224 228. In common carp, t he first aa of t his mot if is a phenylalanine inst ead of t ryosine as goldfish GH R [ 10] . So t he mot if t urns to a F GEFS mot if ac cordingly.…”

Section: Discussionmentioning

confidence: 99%

“…T he conservation of ex tracellular domain partly explains w hy fish GHRs can recog nize GH s of other species [ 10,11] .…”

Section: Discussionmentioning

confidence: 99%

“…U p t o date GHR cDNAs have been cloned from many species [ 7 9] , including various kinds of mamm alian anim als; av ian of chicken and domestic pig eon; rept ilian of soft shelled turt le ( Pelodiscus si nensis j ap onicus ) and amphibian of African clawed frog ( Xenop us laev is) . In the year of 2001, Lee et al cloned the f ull length cDNA of GHR from goldfish ( Car assius aurat us ) [ 10] . T his is t he f irst report about t he cloning of GH R in teleost f ish.…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning of growth hormone receptor (GHR) from common carp (Cyprinus carpio L.) and identification of its two forms of mRNA transcripts

Sun¹,

Qionglin²,

Hu³

et al. 2006

TPNS

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The cD NA of growt h hormone recept or ( GH R) w as cloned f rom t he liver of 2 year common carp ( Cypr inu s carpi o L. ) by reverse t ranscript ion polymerase chain react ion ( R T PCR ) and rapid amplification of cDN A end ( RA CE) . It s open reading frame ( O RF) of 1806 nucleot ides is t ranslated into a put at ive pept ide of 602 amino acids, including an extracellular ligand bin ding domain of 244 amino acids ( aa) , a single t ransmembrane domain of 24 aa and an int racellular signal transduct ion domain of 334 aa. Sequence analysis indi cated t hat common carp G HR is highly homologous t o goldf ish ( Car assi us aurat us) G HR at bot h gene and prot ein levels. U sing a pair of gen e specific primers, a G HR fragment w as amplified from t he cD NA of 2 year common carp, a 224 bp product w as identif ied in liver and a 321 bp product in ot her t issues. T he sequencing of the product s and t he partial genomic DN A indicat ed t hat the diff erence in product size w as t he result of a 97 bp intron t hat alt ernat ively spliced. In addit ion, t he 321 bp f ragm ent could be amplified f rom all t he tissues of 4 mont h common carp in cluding liver, demonst rat ing the occurrence of t he alternat ive splicing of t his int ron during t he development of com mon carp. M oreover, a semi quantit at ive RT PCR w as performed t o analyze t he expression level of G HR in t issues of 2 year common carp and 4 mont h common carp. T he result revealed that in t he tissues of gill, t hymus and brain, t he expression level of GHR in 2 year com mon carp w as sign if icant ly low er t han t hat of 4 mont h common carp.Keywords: growth hormone receptor ( GHR) , molecul ar cl oni ng, transcript, expressi on analysis, common carp.Growt h hormone receptor ( GH R) belongs t o the hem at opo ietic recept or superfam ily [ 1] . T he act ion of g row t h hormone ( GH ) in reg ulat ing g row t h [ 2] , re product ion [ 3] and im munit y [ 4] has been elucidated. T he binding of GH to the GHR on t arget t issues trig gers a cascade of tyrosine and prot ein phosphorylation events, w hich culm inat es in t he biolog ical action of GH [ 5,6] . U p t o date GHR cDNAs have been cloned from many species [ 7 9] , including various kinds of mamm alian anim als; av ian of chicken and domestic pig eon; rept ilian of soft shelled turt le ( Pelodiscus si nensis j ap onicus ) and amphibian of African clawed frog ( Xenop us laev is) . In the year of 2001, Lee et al. cloned the f ull length cDNA of GHR from goldfish ( Car assius aurat us ) [ 10] . T his is t he f irst report about t he cloning of GH R in teleost f ish. Subsequent ly T se successf ully cloned t he GHR cDNA f rom black seabream ( A canthop agr us schl egel ii ) , and found t wo kinds of GHR cDNAs in all the t issues, w hich result ed f rom a 93 bp intron alt ernat ively spliced [ 11] . T he diversity of GH R has been proved in mammals. For ex am ple, 8 GHR mRNAs ( V1 V8) have been ident i fied in human [ 12] , 10 dif ferent GHR cDNAs w ere cloned from a cow endomet rium...

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Calcitonin Gene-Related Peptide (CGRP), a New Endocrine Factor Involved in the Complex Multi-hormonal Control of the Gill Osmoregulation: Evidence from Studies in the European Eel, Anguilla anguilla

Lafont

Dufour

Fouchereau-Péron

2009

Spawning Migration of the European Eel

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Molecular cloning of a teleost growth hormone receptor and its functional interaction with human growth hormone

Cited by 82 publications

References 27 publications

Identification of the growth hormone receptor in an advanced teleost, the tilapia (Oreochromis mossambicus) with special reference to its distinct expression pattern in the ovary

Identification of the growth hormone receptor in an advanced teleost, the tilapia (Oreochromis mossambicus) with special reference to its distinct expression pattern in the ovary

Molecular cloning of growth hormone receptor (GHR) from common carp (Cyprinus carpio L.) and identification of its two forms of mRNA transcripts

Calcitonin Gene-Related Peptide (CGRP), a New Endocrine Factor Involved in the Complex Multi-hormonal Control of the Gill Osmoregulation: Evidence from Studies in the European Eel, Anguilla anguilla

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