Molecular Cloning of a Novel Apoptosis-Related Gene, Human Nap1 (NCKAP1), and Its Possible Relation to Alzheimer Disease

Suzuki, Takashi; Nishiyama, Kazutoshi; Yamamoto, Ayako; Inazawa, J; Iwaki, Toru; Yamada, Takeshi; Kanazawa, Ichiro; Sakaki, Yoshiyuki

doi:10.1006/geno.1999.6053

Cited by 51 publications

(28 citation statements)

References 38 publications

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“…Similarly, Haslinger et al (38) have described HEM1 overexpression in trisomy 12 subgroups. HEM2, a homologue of HEM1 that is expressed only in the brain, when down-regulated with antisense treatment, induces apoptosis in neurons (39). This observation also prompted us to use an antisense against HEM1.…”

Section: Discussionmentioning

confidence: 99%

ATM, CTLA4, MNDA, andHEM1in High versus Low CD38–Expressing B-Cell Chronic Lymphocytic Leukemia

Joshi

Hegde

Dickinson

et al. 2007

Clinical Cancer Research

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Purpose: In B-cell chronic lymphocytic leukemia (CLL), high CD38 expression has been associated with unfavorable clinical course, advanced disease, resistance to therapy, shorter time to first treatment, and shorter survival. However, the genes associated with CLL patient subgroups with high and low CD38 expression and their potential role in disease progression is not known. Experimental Design: To identify the genes associated with the clinical disparity in CLL patients with high versus low CD38 expression, transcriptional profiles were obtained from CLL cells from 39 different patients using oligonucleotide microarray. Gene expression was also compared between CLL cells and B cells from healthy individuals. Results: Gene expression analysis identified 76 differentially expressed genes in CD38 high versus low groups. Out of these genes, HEM1, CTLA4, and MNDA were selected for further studies and their differential expression was confirmed by real-time PCR. HEM1 overexpression was associated with poor outcome, whereas the overexpression of CTLA4 and MNDA was associated with good outcome. Down-regulation of HEM1 expression in patient CLL cells resulted in a significant increase in their susceptibility to fludarabine-mediated killing. In addition, when gene expression patterns in CD38 high and low CLL cells were compared with normal B-cell profiles, ATM expression was found to be significantly lower in CD38 high compared with CD38 low CLL as confirmed by real-time reverse transcription-PCR. Conclusions: These results identify the possible genes that may be involved in cell proliferation and survival and, thus, determining the clinical behavior of CLL patients expressing high or low CD38.

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Section: Discussionmentioning

confidence: 99%

ATM, CTLA4, MNDA, andHEM1in High versus Low CD38–Expressing B-Cell Chronic Lymphocytic Leukemia

Joshi

Hegde

Dickinson

et al. 2007

Clinical Cancer Research

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“…1B). NCKAP1 has been shown to interact with activated Rac, and to be downregulated in the brains of Alzheimer's disease patients (Kitamura et al 1997;Suzuki et al 2000). Drosophila HEM2/NAP1/KETTE is required for axonal pathfinding and for appropriate actin cytoarchitecture.…”

Section: Gex-3 Is Homologous To Hem2/nap1/nckap1 a Mammalian Rac1 Inmentioning

confidence: 99%

The GEX-2 and GEX-3 proteins are required for tissue morphogenesis and cell migrations in C. elegans

Soto¹,

Qadota²,

Kasuya³

et al. 2002

Genes Dev.

123

181

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During body morphogenesis precisely coordinated cell movements and cell shape changes organize the newly differentiated cells of an embryo into functional tissues. Here we describe two genes, gex-2 and gex-3, whose activities are necessary for initial steps of body morphogenesis in Caenorhabditis elegans. In the absence of gex-2 and gex-3 activities, cells differentiate properly but fail to become organized. The external hypodermal cells fail to spread over and enclose the embryo and instead cluster on the dorsal side. Postembryonically gex-3 activity is required for egg laying and for proper morphogenesis of the gonad. GEX-2 and GEX-3 proteins colocalize to cell boundaries and appear to directly interact. GEX-2 and GEX-3 are highly conserved, with vertebrate homologs implicated in binding the small GTPase Rac and a GEX-3 Drosophila homolog, HEM2/NAP1/KETTE, that interacts genetically with Rac pathway mutants. Our findings suggest that GEX-2 and GEX-3 may function at cell boundaries to regulate cell migrations and cell shape changes required for proper morphogenesis and development.

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“…The sizes of the amplified products for h-BD-2 and h-BD-1 are 241 and 258 bp, respectively. For the analysis of Nckap1 (Nap1), cDNAs were amplified for 21 cycles using the primers and conditions described in (48). The size of the amplified product was 184 bp.…”

Section: Northern Blotting and Rt-pcr Analysismentioning

confidence: 99%

Pseudomonas aeruginosa Flagellin and Alginate Elicit Very Distinct Gene Expression Patterns in Airway Epithelial Cells: Implications for Cystic Fibrosis Disease

Cobb

Mychaleckyj

Wozniak

et al. 2004

The Journal of Immunology

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Infection with the opportunistic pathogen Pseudomonas aeruginosa remains a major health concern. Two P. aeruginosa phenotypes relevant in human disease include motility and mucoidy. Motility is characterized by the presence of flagella and is essential in the establishment of acute infections, while mucoidy, defined by the production of the exopolysaccharide alginate, is critical in the development of chronic infections, such as the infections seen in cystic fibrosis patients. Indeed, chronic infection of the lung by mucoid P. aeruginosa is a major cause of morbidity and mortality in cystic fibrosis patients. We have used Calu-3 human airway epithelial cells to investigate global responses to infection with motile and mucoid P. aeruginosa. The response of airway epithelial cells to exposure to P. aeruginosa motile strains is characterized by a specific increase in gene expression in pathways controlling inflammation and host defense. By contrast, the response of airway epithelia to the stimuli presented by mucoid P. aeruginosa is not proinflammatory and, hence, may not be conducive to the effective elimination of the pathogen. The pattern of gene expression directed by flagellin, but not alginate, includes innate host defense genes, proinflammatory cytokines, and chemokines. By contrast, infection with alginate-producing P. aeruginosa results in an overall attenuation of host responses and an antiapoptotic effect.

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Molecular Cloning of a Novel Apoptosis-Related Gene, Human Nap1 (NCKAP1), and Its Possible Relation to Alzheimer Disease

Cited by 51 publications

References 38 publications

ATM, CTLA4, MNDA, andHEM1in High versus Low CD38–Expressing B-Cell Chronic Lymphocytic Leukemia

ATM, CTLA4, MNDA, andHEM1in High versus Low CD38–Expressing B-Cell Chronic Lymphocytic Leukemia

The GEX-2 and GEX-3 proteins are required for tissue morphogenesis and cell migrations in C. elegans

Pseudomonas aeruginosa Flagellin and Alginate Elicit Very Distinct Gene Expression Patterns in Airway Epithelial Cells: Implications for Cystic Fibrosis Disease

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