Molecular cloning, expression and polymorphism of the porcine apolipoprotein A5 gene in a Jinhua × Pietrain F2 reference population

Abstract

As a newly described member of the apolipoprotein gene family, apolipoprotein A5 (APOA5) has been suggested to play a key role in the triglyceride metabolism in both human and mice. The aim of this study was to identify the porcine (Sus scrofa) APOA5 gene, determine its mRNA and its mutations that are associated with lipid accumulation. The porcine APOA5 cDNA was amplified by reverse transcriptase polymerase chain reaction using the information of the mouse or other mammals. It had been determined that the open reading frame of the porcine APOA5 gene consists of 1092 bp, which encodes a predicted protein composed of 363 amino acids with a similarity to bovine (80.43%) and to human (78.47%). The expression analysis indicated that the porcine APOA5 gene was expressed in hypophysis, fat and liver. Twelve single nucleotide polymorphisms (SNPs), including 4 SNPs in the 5 0 end, 1 SNP in second intron, 1 SNP in third exon and 6 SNPs in the 3 0 end, were identified in the porcine APOA5 gene and genotyped on the Jinhua 3 Pietrain F 2 reference population, it had revealed that the SNP of C1834T was significantly associated with average backfat thickness and leaf fat weight ( P , 0.01 and P , 0.05, respectively). In conclusion, this study has got basic information of the porcine APOA5 gene and provides evidence that the APOA5 gene could be a potential candidate gene for fat deposition.

Identification of Single Nucleotide Polymorphism and Pathway Analysis of Apolipoprotein A5 (APOA5) Related to Fatty Acid Traits in Indonesian Sheep

“…In animal, the significant association were reported between SNPs in T635C with abdominal fat weight in F2 cross of White Plymouth Rock x Silkies chicken (Yao et al, 2008). Jiang et al (2010) confirmed in pig that the significant relationship of SNPs in APOA5 gene were found with body fat deposition in F2 Jinhua x Pietrain population.Furthermore, genome wide association study revealed that APOA5 could be a positional candidate gene for fat deposition in Broiler chickens (Moreira et al, 2018). Three genes (APOA1, APOA4, and APOA5) belong to a gene family (Apolipoproteins -APO) that encodes important regulators of lipid biosynthesis and metabolism (Delgado-Lista et al, 2010).…”

Testosterone Deficiency Induces Changes of the Transcriptomes of Visceral Adipose Tissue in Miniature Pigs Fed a High-Fat and High-Cholesterol Diet

“…Therefore, enhanced APOE under testosterone deficiency in our data might amplify castration-induced VAT deposition under HFC diet. Actually, expression levels of other well-known lipoproteins for modulating triglyceride metabolism, such as APOA5 and APOC3 [29,30], were also upregulated under testosterone deficiency, although the expressions of APOC3 were not detected in IM pig groups (Table S1). In the previous study, APOE, APOC3, and APOA5 were shown to be positively associated with plasma triglyceride levels [31,32], which indicated VAT accumulation.…”

Non-Synonymous SNP in theApoRGene Associated with Pork Meat Quality

“…Previous studies have reported that such triglyceriderich lipoproteins as VLDL and chylomicron primarily transported fatty acids as triglycerides to extra-hepatic tissues where they were stored or oxidized for energy production and therefore had critical effects on the lipid metabolism related to meat quality traits. 1,7,8) ApoR is known to be a component of VLDL and chylomicrons and to contain two short consensus repeats (SCRs) 2) which play an important role as a common protein interaction module. 9) chr9:g.132356063C > T SNP in this study was located within an SCR region of ApoR and created single base mutations (TCC !…”

“…Some SNPs in ApoVLDL genes, encoding apolipoproteins for VLDL, have been similarly identified to have a significant association with meat quality traits; for example, C1834T SNP, which is located in 3 0 -UTR of the ApoA5 gene, has shown a significant association with the backfat thickness and leaf fat weight. 7) Intronic SNP in ApoVLDL-II gene has also been associated with multiple meat quality traits, including the intramuscular fat content. 1) SNPs in the ApoVLDL genes are therefore suggested to have broad effects on meat quality traits, although there have been few non-synonymous exonic SNPs which may influence the structural architechure of ApoVLDL.…”