Molecular cloning, expression and functional characterization of interferon-γ-inducible lysosomal thiol reductase (GILT) gene from mandarin fish (Siniperca chuatsi)

Song, Jiuzhou; Liu, Hongzhen; Ma, Lei; Ma, Lixin; Gao, Cuixiang; Zhang, Shuangquan

doi:10.1016/j.fsi.2014.03.021

Cited by 12 publications

(2 citation statements)

References 28 publications

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“…In mammals, the gamma-interferon-inducible lysosomal thiol reductase (GILT) is involved in the processing and presentation of antigens into the major histocompatibility complex (MHC) class II [13]. Similar functions have also been described for several orthologous fish genes [14][15][16][17][18][19][20][21]. Furthermore, it has been described that GILT controls the replication of murine leukaemia virus and human immunodeficiency virus type 1 [22,23]; therefore, the gilt downregulation detected in wt160-infected animals could contribute to increase viral virulence.…”

Section: Discussionmentioning

confidence: 80%

Immune Response of Senegalese Sole against Betanodavirus Mutants with Modified Virulence

et al. 2021

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Nervous necrosis virus (NNV), genus Betanodavirus, the etiological agent of the viral encephalopathy and retinopathy (VER), presents a genome with two positive-sense single-stranded RNA segments. Striped jack nervous necrosis virus (SJNNV) and red-spotted grouper nervous necrosis virus (RGNNV), together with reassortants RGNNV/SJNNV, are the betanodaviruses predominantly isolated in Southern Europe. An RGNNV/SJNNV reassortant isolated from Senegalese sole (wt160) causes high mortalities in this fish species. This virus presents differences in the sequence of the 3’ non-coding region (NCR) of both segments compared to RGNNV and SJNNV reference strains. Previously, it has been reported that the reversion of two of these differences (nucleotides 1408 and 1412) in the RNA2 3’NCR to the SJNNV-type (recombinant r1408-1412) resulted in a decrease in sole mortality. In the present study, we have applied an OpenArray® to analyse the involvement of sole immune response in the virulence of several recombinants: the r1408-1412 and two recombinants, developed in the present study, harbouring mutations at positions 3073 and 3093 of RNA1 3’NCR to revert them to RGNNV-type. According to the correlation values and to the number of expressed genes, the infection with the RNA2-mutant provoked the most different immune response compared to the immune response triggered after the infection with the rest of the viruses, and the exclusive and high upregulation of genes related to the complement system. The infection with the RNA1-mutants also provoked a decrease in mortality and their replication was delayed at least 24 h compared to the wt160 replication, which could provoke the lag observed in the immune response. Furthermore, the infection with the RNA1-mutants provoked the exclusive expression of pkr and the downregulation of il17rc.

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Section: Discussionmentioning

confidence: 80%

Immune Response of Senegalese Sole against Betanodavirus Mutants with Modified Virulence

et al. 2021

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“…In zebrafish, purified recombinant GILT was capable of catalyzing the reduction of protein disulfide bonds [89]. GILT was constitutively expressed in several tissues of zebrafish, large yellow croaker and mandarin fish (Siniperca chuatsi) and upregulated in the spleen and kidney after stimulation [89][90][91].…”

Section: Peptide Processing Loading and Transport By Mhc In Teleost Fishmentioning

confidence: 99%

Antigen Presentation and Autophagy in Teleost Adaptive Immunity

Johnstone

Chaves-Pozo

2022

IJMS

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Infectious diseases are a burden for aquaculture. Antigen processing and presentation (APP) to the immune effector cells that fight pathogens is key in the adaptive immune response. At the core of the adaptive immunity that appeared in lower vertebrates during evolution are the variable genes encoding the major histocompatibility complex (MHC). MHC class I molecules mainly present peptides processed in the cytosol by the proteasome and transported to the cell surface of all cells through secretory compartments. Professional antigen-presenting cells (pAPC) also express MHC class II molecules, which normally present peptides processed from exogenous antigens through lysosomal pathways. Autophagy is an intracellular self-degradation process that is conserved in all eukaryotes and is induced by starvation to contribute to cellular homeostasis. Self-digestion during autophagy mainly occurs by the fusion of autophagosomes, which engulf portions of cytosol and fuse with lysosomes (macroautophagy) or assisted by chaperones (chaperone-mediated autophagy, CMA) that deliver proteins to lysosomes. Thus, during self-degradation, antigens can be processed to be presented by the MHC to immune effector cells, thus, linking autophagy to APP. This review is focused on the essential components of the APP that are conserved in teleost fish and the increasing evidence related to the modulation of APP and autophagy during pathogen infection.

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Molecular cloning, expression and functional characterization of interferon-γ-inducible lysosomal thiol reductase (GILT) gene from mandarin fish (Siniperca chuatsi)

Cited by 12 publications

References 28 publications

Immune Response of Senegalese Sole against Betanodavirus Mutants with Modified Virulence

Immune Response of Senegalese Sole against Betanodavirus Mutants with Modified Virulence

Antigen Presentation and Autophagy in Teleost Adaptive Immunity

Transcriptome analysis of red sea bream (Pagrus major) head kidney and spleen infected by Vibrio anguillarum

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