Molecular cloning, expression and chromosomal localization of a novel human REG family gene, REG III

Nata, Koji; Liu, Yi; Xu, Liang; Ikeda, Takayuki; Akiyama, Takako; Noguchi, Naoya; Kawaguchi, Shoko; Yamauchi, Akiyo; Takahashi, Ikuo; Shervani, Nausheen J.; Onogawa, Tohru; Takasawa, Shin; Okamoto, Hiroshi

doi:10.1016/j.gene.2004.06.010

Cited by 65 publications

(34 citation statements)

References 32 publications

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“…However, the correlations reported among serum markers (CRP and IL-6, IL-6, and IL-10) are a proof of concept of their transcendental role upon the inflammatory network aroused during AP, as has been previously reported by other authors [5,21,22]. It was unexpected not to find any association between IL-6 and PAP, because experimental models have shown response elements for IL-6 in the gene codifying for PAP; thus high levels of IL-6 would induce stimulation of PAP gene transcription [11,23]. This lack of correlation may be explained by the different timing for each marker to reach its peak serum concentration.…”

Section: Discussionsupporting

confidence: 70%

Time Frames for Analysis of Inflammatory Mediators in Acute Pancreatitis: Improving Admission Triage

et al. 2008

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Improving the outcome of acute pancreatitis through prognostic markers has been a matter of ample research. We evaluate the clinical usefulness of four serum markers in comparison to Ranson's score. Serum measurements of C-reactive protein (CRP), interleukin-6, -10 (IL-6, IL-10), and pancreatitis-associated protein (PAP) were performed. The usefulness of each marker for predicting severity was compared with that of Ranson's score. Time of evolution was considered for improving their usefulness. Seventy-one patients were studied. Severe cases had higher levels of all markers, although only IL-10 had better accuracy than Ranson's. In patients admitted during the first 48 h, IL-6, IL-10, and PAP had improved accuracy over Ranson's; however, after this time frame, only CRP outperformed Ranson's score. Analysis of time frames improved the accuracy of all markers. Therefore, time of evolution should be considered when using these parameters for a better prognosis.

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Section: Discussionsupporting

confidence: 70%

Time Frames for Analysis of Inflammatory Mediators in Acute Pancreatitis: Improving Admission Triage

et al. 2008

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“…The antimicrobial proteins REG3B and REG3G are secreted C-type lectins (Vaishnava et al, 2011) that are predominantly expressed in the gastrointestinal tract, but not in the liver, under homeostatic conditions (Nata et al, 2004). REG3G has bactericidal activity against Gram-positive bacteria and helps maintain the spatial segregation of luminal bacteria and the intestinal epithelial surface (Cash et al, 2006; Mukherjee et al, 2014; Vaishnava et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Intestinal REG3 Lectins Protect against Alcoholic Steatohepatitis by Reducing Mucosa-Associated Microbiota and Preventing Bacterial Translocation

Wang

Fouts

Stärkel

et al. 2016

Cell Host & Microbe

307

281

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Summary Approximately half of all deaths from liver cirrhosis, the 10th leading cause of mortality in the United States, are related to alcohol use. Chronic alcohol consumption is accompanied by intestinal dysbiosis and bacterial overgrowth, yet little is known about the factors that alter the microbial composition or their contribution to liver disease. We previously associated chronic alcohol consumption with lower intestinal levels of the antimicrobial-regenerating islet-derived (REG)-3 lectins. Here, we demonstrate that intestinal deficiency in REG3B or REG3G increases numbers of mucosa-associated bacteria and enhances bacterial translocation to the mesenteric lymph nodes and liver, promoting the progression of ethanol-induced fatty liver disease toward steatohepatitis. Overexpression of Reg3g in intestinal epithelial cells restricts bacterial colonization of mucosal surfaces, reduces bacterial translocation, and protects mice from alcohol-induced steatohepatitis. Thus, alcohol appears to impair control of the mucosa-associated microbiota, and subsequent breach of the mucosal barrier facilitates progression of alcoholic liver disease.

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“…Reg proteins are members of Reg (Regenerating gene) multigene family [11] [12] [13], classified as C-type lectins that exhibit carbohydrate specificity via C-type lectin like domain (CTLD) [14]. Class III Reg family proteins show antibacterial activity against Salmonella Typhimurium, Yersinia pseudituberculosis, Bacillus subtilis, Enterococcus faecalis, Listeria innocua and L. monocytogens [15]- [20] owing to their ability to bind to molecules displayed on bacterial cell [22].…”

Section: Introductionmentioning

confidence: 99%

Recombinant Human REG I<i>α</i> Aggregates <i>Staphylococcus aureus</i>—Exhibits a Lectin-Like Function

Jamal¹,

Kezuka²,

Nonaka³

et al. 2017

ABB

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Staphylococcus aureus is pathogenic to humans with worldwide health care concern due to its ability to evade the immune system and develop resistance to multiple drugs. Reg family proteins are C-type lectins with antimicrobial properties. Bacterial aggregation through binding to microbial cell surface sugar and/or lipid moieties is key mechanism employed in the process. In the present study we have analysed the antimicrobial effect of human REG Iα on S. aureus. Aggregation of mid-log phase culture of S. aureus was observed in presence of purified recombinant REG Iα. Therefore REG Iα can be applied in eliminating S. aureus infections in humans.

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Molecular cloning, expression and chromosomal localization of a novel human REG family gene, REG III

Cited by 65 publications

References 32 publications

Time Frames for Analysis of Inflammatory Mediators in Acute Pancreatitis: Improving Admission Triage

Time Frames for Analysis of Inflammatory Mediators in Acute Pancreatitis: Improving Admission Triage

Intestinal REG3 Lectins Protect against Alcoholic Steatohepatitis by Reducing Mucosa-Associated Microbiota and Preventing Bacterial Translocation

Recombinant Human REG I<i>α</i> Aggregates <i>Staphylococcus aureus</i>—Exhibits a Lectin-Like Function

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Molecular cloning, expression and chromosomal localization of a novel human REG family gene, REG III

Cited by 65 publications

References 32 publications

Time Frames for Analysis of Inflammatory Mediators in Acute Pancreatitis: Improving Admission Triage

Time Frames for Analysis of Inflammatory Mediators in Acute Pancreatitis: Improving Admission Triage

Intestinal REG3 Lectins Protect against Alcoholic Steatohepatitis by Reducing Mucosa-Associated Microbiota and Preventing Bacterial Translocation

Recombinant Human REG I&lt;i&gt;α&lt;/i&gt; Aggregates &lt;i&gt;Staphylococcus aureus&lt;/i&gt;—Exhibits a Lectin-Like Function

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Recombinant Human REG I<i>α</i> Aggregates <i>Staphylococcus aureus</i>—Exhibits a Lectin-Like Function