Molecular Cloning and Immunologic Characterization of a Novel cDNA Coding for Progesterone-Induced Blocking Factor

Polgar, Beata; Kispál, Gyula; Lachmann, Margit; Paar, Gabriella; Nagy, Eszter; Csere, Péter; Mikó, Éva; Szereday, László; Варга, П.; Szekeres-Barthó, Júlia

doi:10.4049/jimmunol.172.4.2704

Cited by 16 publications

(35 citation statements)

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“…The abundance and predominance of the 90 kDa PIBF protein on Western blots of cell lines 5 and tumor tissues (Fig. 4) as well as its copurification with centrosomes suggested that it was mainly the full-length PIBF that was detected in the perinuclear dots by the anti-PIBF-exon17 antibody in the immunofluorescent studies.…”

Section: Subcellular Localization Of Pibfmentioning

confidence: 96%

“…1 However, previous protein expression studies revealed that PIBF was also expressed by the progesterone receptor positive human mammary carcinoma cell line, MCF-7. 5 An extensive BLAST search analysis of the cloned PIBF mRNA sequence using the human dEST library database (http://www.ncbi.nlm.nih.gov/ BLAST, est_human database) suggested a more universal expression and revealed that PIBF mRNA is expressed not only in lymphocytes but also in many other cell types, mainly in embryonic tissues, pregnant uterus, placenta and testis (ϳ 30% of all entries). PIBF mRNA was most frequently (50% of all entries) identified in cDNA libraries prepared from human malignant tumors with different tissue origin (only entries containing at least 2 exons of the PIBF mRNA sequence or the last exon with polyA tail were included).…”

Section: Overexpression Of Pibf Mrna In Malignant Tumorsmentioning

confidence: 99%

“…Northern blot analysis of normal human tissues ascertained the presence of an approximately 3,200 nt long mRNA (data not shown), which was the major RNA band and was compatible with the size predicted from the cloned cDNA (GenBank Account number NM006346). 5 Each of the 23 normal adult tissues tested (using commercially available premade Northern blots purchased from Clontech; 7760-1, 7766-1 and7767-1) showed expression of the PIBF mRNA at variable but detectable levels. The highest signal was detected with RNA samples isolated from testis, followed by thyroid tissue (data not shown).…”

Section: Overexpression Of Pibf Mrna In Malignant Tumorsmentioning

confidence: 99%

“…[1][2][3][4] Recently, evidence has emerged that PIBF is expressed by malignant cells as it was detected in the culture supernatant of the human mammary carcinoma cell line MCF-7 upon induction with insulin. 5 This suggests that PIBF produced by a tumor cell line might be secreted similarly to pregnancy or activated lymphocytes in culture. In addition to the secreted form of PIBF, a higher molecular weight intracellular isoform was detected in MCF-7 cells independently of progesterone or insulin stimulation.…”

mentioning

confidence: 99%

“…The size of the intracellular PIBF protein is 90 kDa and corresponds to that predicted by the open reading frame of the PIBF mRNA identified during the cloning of the gene. 5 Recent genetic studies indicated pibf among the candidate genes for breast cancer predisposition and cancer progression in the 13q21-q22 chromosomal region. 6 Considering its immunosuppressive effects, PIBF secreted by undifferentiated, malignant cells may contribute to tumor development.…”

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confidence: 99%

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PIBF (progesterone induced blocking factor) is overexpressed in highly proliferating cells and associated with the centrosome

Lachmann

Gelbmann

Kálmán

et al. 2004

Intl Journal of Cancer

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103

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PIBF was previously identified as a 34 kDa immunomodulatory molecule secreted by pregnancy lymphocytes and is thought to play a crucial role in preventing rejection of the embryo by the maternal immune response. Recent data suggested that PIBF protein was also expressed by the progesterone receptor (PR) positive MCF-7 breast tumor cell line. Given the notion that a number of proteins shown to be involved in tumorigenesis are associated with the centrosome and disturbed centrosome function causes unequal segregation of chromosomes, studies to evaluate whether or not PIBF that is highly expressed in tumors is directly involved in tumorigenesis are thus warranted.

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Section: Subcellular Localization Of Pibfmentioning

confidence: 96%

Section: Overexpression Of Pibf Mrna In Malignant Tumorsmentioning

confidence: 99%

Section: Overexpression Of Pibf Mrna In Malignant Tumorsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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PIBF (progesterone induced blocking factor) is overexpressed in highly proliferating cells and associated with the centrosome

Lachmann

Gelbmann

Kálmán

et al. 2004

Intl Journal of Cancer

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103

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Evidence that exposure to progesterone alone is a sufficient stimulus to cause a precipitous rise in the immunomodulatory protein the progesterone induced blocking factor (PIBF)

Cohen

Check

Dougherty

2015

J Assist Reprod Genet

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Purpose To determine if exposure to progesterone alone is sufficient to increase the production of the immunomodulatory protein known as the progesterone induced blocking factor (PIBF). Also to determine what method of progesterone delivery or form of P best stimulates PIBF secretion. Methods Serum samples from patients with infertility and paid volunteers were evaluated for both PIBF and progesterone at various times during the follicular phase and the luteal phase in both natural cycles and cycles involving embryo transfer after endogenous and exogenous progesterone exposure and after various synthetic progestins. PIBF was measured by a non-commercial research ELISA assay. Comparisons were made of serum PIBF before and after exposure to progesterone, 17-hydroxyprogesterone, and oral contraceptives. PIBF was also measured before and after transfer of embryos.Results Progesterone alone without exposure to the fetal allogeneic stimulus was able to produce a marked increase in s e r u m P I B F. N e i t h e r a s y n t h e t i c p r o g e s t i n (19-nortestosterone derivative) nor 17-hydroxyprogesterone caused an increase in PIBF. Some PIBF is generally detected even in the follicular phase. Conclusions A previous concept considered that an allogeneic stimulus, e.g., from the fetal semi-allograft, was necessary to induce de novo progesterone receptors in gamma delta T cells, which, in turn, when exposed to a high concentration of progesterone, would secrete high levels of PIBF. These data show that exposure to an allogeneic stimulus is not needed to cause a marked rise in PIBF, merely progesterone alone is sufficient.

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High viral load and deregulation of the progesterone receptor signaling pathway: Association with Hepatitis E-related poor pregnancy outcome

Bose¹,

Das²,

Kumar³

et al. 2011

Journal of Hepatology

125

128

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Molecular Cloning and Immunologic Characterization of a Novel cDNA Coding for Progesterone-Induced Blocking Factor

Cited by 16 publications

References 0 publications

PIBF (progesterone induced blocking factor) is overexpressed in highly proliferating cells and associated with the centrosome

PIBF (progesterone induced blocking factor) is overexpressed in highly proliferating cells and associated with the centrosome

Evidence that exposure to progesterone alone is a sufficient stimulus to cause a precipitous rise in the immunomodulatory protein the progesterone induced blocking factor (PIBF)

High viral load and deregulation of the progesterone receptor signaling pathway: Association with Hepatitis E-related poor pregnancy outcome

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