Molecular cloning and expression of cDNA encoding a murine myeloid leukaemia inhibitory factor (LIF).

Abstract

Leukaemia inhibitory factor (LIF) can induce macrophage differentiation in M1 murine myeloid leukaemic cells and suppress their proliferation in vitro. It does not stimulate the proliferation of normal progenitor cells and is apparently distinct from known colony‐stimulating factors. We have used oligo‐nucleotides complementary to partial amino acid sequence of LIF to isolate a LIF clone from a T lymphocyte cDNA library. When this cDNA was coupled to a yeast expression vector (YEpsec1) and introduced into yeast cells, a molecule with the biological properties characteristic of native LIF was secreted into the growth medium. The amino acid sequence of LIF established it to be a unique molecular entity, distinct from the other known haemopoietic growth factors. Since LIF is encoded by a unique gene, two biochemically separable forms of LIF probably represent post‐transcriptional or posttranslational variants of the same gene product. In contrast to several other haemopoietic regulators, the 0.8‐ to 1‐kb LIF mRNA was expressed constitutively in two murine T lymphocyte cell lines examined, and its abundance was not enhanced by stimulation with concanavalin A. Cloning, sequencing and expressing LIF has resolved several discrepancies in the literature concerning the identity of factors capable of inducing differentiation of murine myeloid leukaemic cells in vitro.

Molecular cloning and functional analysis of cDNA encoding a rat leukemia inhibitory factor: towards generation of pluripotent rat embryonic stem cells

“…It was characterized and cloned by its ability to induce the di erentiation of the murine myeloid leukemia cell line M1 , Gearing et al, 1987. Although initially characterized by its di erentiation-inducing e ect on myeloid leukemic cells, LIF also displays a number of other biological activities (Gough and Williams, 1989).…”

Estudo experimental comparativo da ação das neurocinas cardiotrofina-1 e oncostatina-m na regeneração nervosa periférica

“…Previous studies (ADLER et al, 1979;GEARING et al, 1987;YAMAMORI et al, 1989;RICHARDSON, 1994;PEREIRA, 1998;MAJITHIA, 1998) demonstrated that CNTF and LIF stimulated nerve regeneration after their administration on lesion of adult mice sciatic nerves.…”

“…Também fazem parte deste grupo, CNTF, LIF, GPA e as interleucinas 6 e 11 (IL-6/IL11) (HOPKINS; ROTHWELL, 1995). Nos chamou a atenção que em estudos prévios (ADLER et al, 1979;GEARING et al, 1987;YAMAMORI et al, 1989;RICHARDSON, 1994;PEREIRA, 1998;MAJITHIA, 1998) o CNTF e o LIF estimularam a regeneração nervosa após a sua administração sobre a lesão de nervos ciáticos de camundongos adultos.…”

Free LIF receptor α-chain distal cytoplasmic motifs enhance Jak2-independent STAT3 phosphorylation and induce differentiation in HL-60 cells

“…the leukemia inhibitory factor (lIF) has been cloned and purified on the basis of its capacity to induce differentiation of the M1 murine myeloid leukemia cell line (1,2). As a member of the Il-6 family of cytokines, lIF mediates its biological effects by binding to its receptor, which consists of a lowaffinity lIFr α-chain termed lIFr α and a high-affinity subunit termed gp130, both of which are found in other Il-6 family members (3).…”