Molecular cloning and characterization of the full-length Hsp90 gene from Matricaria recutita

Abstract

ABSTRACT. Heat shock protein 90 (Hsp90) is one of the most abundant and conserved chaperone proteins and plays important roles in plant growth and responses to environmental stimuli. However, little is known regarding the sequence and function of Hsp90s in Matricaria recutita. In the present study, we cloned the full-length cDNA sequence of the hsp90 gene from this species. Using rapid amplification of cDNA ends technologies with 2 degenerate primers that were designed based on the hsp90 gene sequence from other members of Asteraceae, we isolated and characterized an Hsp90 homolog gene from M. recutita (Mr-Hsp90). The full-length Mr-hsp90 cDNA sequence, containing 2097 base pairs, encodes a protein of 698 amino acids. Based on amino acid sequence identity, Mr-Hsp90 showed high similarity to other cloned Hsp90 proteins. The Mr-Hsp90 protein was closely clustered with the Lactuca sativa in a phylogenetic tree. These results indicate that the cloned sequence of Mr-Hsp90 is a member of the Hsp90 family, which is reported for the first time in M. recutita. Next, we conducted a salt stress experiment to determine the protein's function under salt stress conditions. Survival of chamomile seedlings subjected to heat-shock pretreatment was significantly increased compared with groups that had not undergone heat-shock pretreatment in a salt stress 10995 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (4): 10994-11003 (2014) Cloning and characterization of environment. This indicates that Mr-Hsp90 plays an important role in the salt resistance of chamomile seedlings.

Tonsillectomy and the Risk for Deep Neck Infection—A Nationwide Cohort Study

“…Many studies on the effects of tonsillectomy on the immune demonstrate discordant results. Some studies indicated that tonsillectomy decreases the serum immunoglobulin level during some or all of their follow-up periods[ 14 , 27 – 30 ] or alters cellular immunity[ 31 ]. In contrast, some studies reveal that tonsillectomies do not alter immune functions [ 15 , 17 , 32 ].…”

Enhanced salt tolerance in tomato plants constitutively expressing heat-shock protein in the endoplasmic reticulum

“…ER-sHSP has the functional activity of a molecular chaperone (Zhao et al, 2007;Mamedov and Shono, 2008) and its overexpression can alleviate ER stress caused by tunicamycin and DTT exposure (Zhao et al, 2007). The aim of this study was to determine whether the constitutive expression of ER-sHSP would improve the abiotic tolerance of plants, as does overexpression of BiP (Alvim et al, 2001;Valente et al, 2009) and an increased HSP90 level (Ling et al, 2014). To this end, we examined salt tolerance in transgenic tomato plants expressing ER-sHSP.…”