Molecular cloning and characterization of a chlorophyll degradation regulatory gene (ZjSGR) from Zoysia japonica

Teng, Ke; Chang, Zhihui; Xiao, G Z; Guo, Weier; Xu, Lixin; Chao, Yuehui; Han, Liebao

doi:10.4238/gmr.15028176

Cited by 7 publications

(8 citation statements)

References 28 publications

(38 reference statements)

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“…The data showed that HAB1 and SNRK2-9 were up-regulated in ABA signal transduction, reflecting a possible negative feedback regulation of ABA metabolism (Wang, 2015), and ABA mediate pathway for stress response in ZjSGR -overexpressing plants (Fujii et al, 2011). This is consistent with our previous study that overexpression of SGR drastically increased the ABA contents in tobacco (Teng et al, 2015). ERF1 and ERF2 are biosynthetic and responsive factors involving in ethylene pathway (Tsai et al, 2014).…”

Section: Discussionsupporting

confidence: 94%

Functional and RNA-Sequencing Analysis Revealed Expression of a Novel Stay-Green Gene from Zoysia japonica (ZjSGR) Caused Chlorophyll Degradation and Accelerated Senescence in Arabidopsis

Teng

Chang

et al. 2016

Front. Plant Sci.

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Senescence is not only an important developmental process, but also a responsive regulation to abiotic and biotic stress for plants. Stay-green protein plays crucial roles in plant senescence and chlorophyll degradation. However, the underlying mechanisms were not well-studied, particularly in non-model plants. In this study, a novel stay-green gene, ZjSGR, was isolated from Zoysia japonica. Subcellular localization result demonstrated that ZjSGR was localized in the chloroplasts. Quantitative real-time PCR results together with promoter activity determination using transgenic Arabidopsis confirmed that ZjSGR could be induced by darkness, ABA and MeJA. Its expression levels could also be up-regulated by natural senescence, but suppressed by SA treatments. Overexpression of ZjSGR in Arabidopsis resulted in a rapid yellowing phenotype; complementary experiments proved that ZjSGR was a functional homolog of AtNYE1 from Arabidopsis thaliana. Over expression of ZjSGR accelerated chlorophyll degradation and impaired photosynthesis in Arabidopsis. Transmission electron microscopy observation revealed that overexpression of ZjSGR decomposed the chloroplasts structure. RNA sequencing analysis showed that ZjSGR could play multiple roles in senescence and chlorophyll degradation by regulating hormone signal transduction and the expression of a large number of senescence and environmental stress related genes. Our study provides a better understanding of the roles of SGRs, and new insight into the senescence and chlorophyll degradation mechanisms in plants.

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Section: Discussionsupporting

confidence: 94%

Functional and RNA-Sequencing Analysis Revealed Expression of a Novel Stay-Green Gene from Zoysia japonica (ZjSGR) Caused Chlorophyll Degradation and Accelerated Senescence in Arabidopsis

Teng

Chang

et al. 2016

Front. Plant Sci.

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“…In Z. japonica , functional analyses of SAGs have been performed by transgenic approaches that introduce them in Arabidopsis. Arabidopsis plants expressing ZjSGR, ZjPPH , and ZjNOL genes that involved in chlorophyll degradation, exhibited rapid chlorophyll degradation and senescence; this phenotype closely resembled functional studies of homologous genes in Arabidopsis ( Teng et al., 2016b ; Teng et al., 2021 ; Guan et al., 2022a ). Taken together, these findings suggest that SAGs have a conserved regulatory role in Arabidopsis and Z. japonica , although the number of genes tested is limited.…”

Section: Discussionsupporting

confidence: 54%

“…However, to date few studies have addressed leaf senescence in Z. japonica , despite the potential economic benefits of studying senescence-related phenotypes—such as visual greenness, nutritional levels, and biomass accumulation—in turfgrass ( Teng et al., 2016a ). Overexpression of ZjSGR, ZjPPH , and ZjNOL genes involved in chlorophyll degradation has been found to result in rapid leaf yellowing and/or early senescence phenotypes in Arabidopsis ( Teng et al., 2016b ; Teng et al., 2021 ; Guan et al., 2022a ). In addition, approximately 200 SAG genes were identified as potential markers of senescence via subtractive hybridization of dark-induced leaf senescence in Z. japonica ( Cheng et al., 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Comprehensive transcriptomic analysis of age-, dark-, and salt-induced senescence reveals underlying mechanisms and key regulators of leaf senescence in Zoysia japonica

et al. 2023

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The lawn grass Zoysia japonica is widely cultivated for its ornamental and recreational value. However, its green period is subject to shortening, which significantly decreases the economic value of Z. japonica, especially for large cultivations. Leaf senescence is a crucial biological and developmental process that significantly influences the lifespan of plants. Moreover, manipulation of this process can improve the economic value of Z. japonica by extending its greening period. In this study, we conducted a comparative transcriptomic analysis using high-throughput RNA sequencing (RNA-seq) to investigate early senescence responses triggered by age, dark, and salt. Gene set enrichment analysis results indicated that while distinct biological processes were involved in each type of senescence response, common processes were also enriched across all senescence responses. The identification and validation of differentially expressed genes (DEGs) via RNA-seq and quantitative real-time PCR provided up- and down-regulated senescence markers for each senescence and putative senescence regulators that trigger common senescence pathways. Our findings revealed that the NAC, WRKY, bHLH, and ARF transcription factor (TF) groups are major senescence-associated TF families that may be required for the transcriptional regulation of DEGs during leaf senescence. In addition, we experimentally validated the senescence regulatory function of seven TFs including ZjNAP, ZjWRKY75, ZjARF2, ZjNAC1, ZjNAC083, ZjARF1, and ZjPIL5 using a protoplast-based senescence assay. This study provides new insight into the molecular mechanisms underlying Z. japonica leaf senescence and identifies potential genetic resources for enhancing its economic value by prolonging its green period.

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“…The Z. japonica beta-actin was used as the internal reference gene (GenBank accession no. GU290546) [54]. Relevant primer sequences used are shown in Table S1.…”

Section: Expression Levels Of the Psy Gene And Pigment-related Genesmentioning

confidence: 99%

Expression of ZjPSY, a Phytoene Synthase Gene from Zoysia japonica Affects Plant Height and Photosynthetic Pigment Contents

Dong

Zhao

Teng

et al. 2022

Plants

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Phytoene synthase (PSY) is a key limiting enzyme in the carotenoid biosynthesis pathway for regulating phytoene synthesis. In this study, ZjPSY was isolated and identified from Zoysia japonica, an important lawn grass species. ZjPSY cDNA was 1230 bp in length, corresponding to 409 amino acids. ZjPSY showed higher expression in young leaves and was downregulated after GA3, ABA, SA, and MeJA treatments, exhibiting a sensitivity to plant hormones. Regulatory elements of light and plant hormone were found in the upstream of ZjPSY CDS. Expression of ZjPSY in Arabidopsis thaliana protein led to carotenoid accumulation and altered expression of genes involved in the carotenoid pathway. Under no-treatment condition, salt treatment, and drought treatment, transgenic plants exhibited yellowing, dwarfing phenotypes. The carotenoid content of transgenic plants was significantly higher than that of wild-type under salt stress and no-treatment condition. Yeast two-hybrid screening identified a novel interacting partner ZjJ2 (DNAJ homologue 2), which encodes heat-shock protein 40 (HSP40). Taken together, this study suggested that ZjPSY may affect plant height and play an important role in carotenoid synthesis. These results broadened the understanding of carotenoid synthesis pathways and laid a foundation for the exploration and utilization of the PSY gene.

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Molecular cloning and characterization of a chlorophyll degradation regulatory gene (ZjSGR) from Zoysia japonica

Cited by 7 publications

References 28 publications

Functional and RNA-Sequencing Analysis Revealed Expression of a Novel Stay-Green Gene from Zoysia japonica (ZjSGR) Caused Chlorophyll Degradation and Accelerated Senescence in Arabidopsis

Functional and RNA-Sequencing Analysis Revealed Expression of a Novel Stay-Green Gene from Zoysia japonica (ZjSGR) Caused Chlorophyll Degradation and Accelerated Senescence in Arabidopsis

Comprehensive transcriptomic analysis of age-, dark-, and salt-induced senescence reveals underlying mechanisms and key regulators of leaf senescence in Zoysia japonica

Expression of ZjPSY, a Phytoene Synthase Gene from Zoysia japonica Affects Plant Height and Photosynthetic Pigment Contents

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