Molecular cloing and bioinformatics analysis of lactate dehydrogenase from Taenia multiceps

Guo, Cheng; Wang, Yu; Huang, Xing; Wang, Ning; Yan, Ming; He, Ran; Gu, Xiaobin; Xie, Yue; Lai, Weimin; Jing, Bo; Peng, Xuerong; Yang, Guangyou

doi:10.1007/s00436-017-5568-x

Cited by 6 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sixteen sheep (6 months old) (From 1 October 2020 to 30 March 2021 in Najaf city) were recruited to perform the experiment, in which eight of them were injected twice at 21-day interval using the cellular metabolic antigen of C. cerebralis protoscolex. The second group of eight sheep served as controls (12). Blood samples were collected from all animals at day-0 (before injection) and at day 10, 18, and 24 after the first injection, and at day 10, 16, 26, 40, 48, 53, 61, 80, 85, and 89 after the second injection.…”

Section: Samples and Experimental Designmentioning

confidence: 99%

Protoscolex metabolites of Coenurus cerebralis as antigenic-produced humoral immune response in sheep

Alkhuzaie¹

2022

IJVS

View full text Add to dashboard Cite

The purpose of the current experiment was to test the immunization against Coenurus cerebralis in sheep. Sixteen animals (6 months old, from 1 October 2020 to 30 March 2021 in Najaf city) were recruited to perform the experiment, in which eight of them were injected twice at 21-day interval using the cellular metabolic antigen of C. cerebralis protoscolex cultivated and then emulsified by complete Freund's adjuvant (CFA). The shots were injected intramuscularly at a dose of 1 ml (15 mg of antigenic protein determined in a separate experiment). The second group of eight sheep served as controls (injected intramuscularly with 1 ml sterile saline only at the days of injections). Blood samples were collected from all animals at day-0 (before injection) and at day 10, 18, and 24 after the first injection, and at day10, 16,26, 40, 48, 53, 61, 80, 85, and 89 after the second injection. Serum activity was studied by indirect enzyme-linked immunosorbent assay (iELISA). The findings, by iELISA, revealed that the cellular antigen of C. cerebralis protoscolices is an active stimulator of antibody response. Day-10 (after the first injection) showed significantly (P<0.05) 3.4 to 9.9 time-higher antibody levels compared to those from day-0. This elevation in the titer of antibodies was increased after receiving the second dose showing 6.3 to 12 time-higher antibody presence even at the final days of blood collection compared to those from day-0. No changes were noticed in the sera of the control animals. The obtained data allow us to conclude that metabolites synthesized by cultivation are active immunogenic components that activate the humoral part of the immune system manifested by the increases in the antibody titers. This gives a solid ground for future work regarding alternative methods of discovering immunization techniques against cestodes.

show abstract

Section: Samples and Experimental Designmentioning

confidence: 99%

Protoscolex metabolites of Coenurus cerebralis as antigenic-produced humoral immune response in sheep

Alkhuzaie¹

2022

IJVS

View full text Add to dashboard Cite

show abstract

“…Adult worms adhere to the small intestine of canids, while the larval stage (coenurus cerebralis) causes coenurosis, a disease affecting herbivores, resulting in great economic loss to animal husbandry in Europe, the USA, Africa and Asia [ 1 – 4 ]. The larval parasites are localized in the central nervous system, as well as subcutaneous and muscle tissue of the intermediate host, most of which are herbivorous animals [ 5 – 7 ], but humans can also act as intermediate hosts if eggs are eaten by mistake [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterisation and expression analysis of two heat-shock proteins in Taenia multiceps

et al. 2019

Self Cite

View full text Add to dashboard Cite

BackgroundTaenia multiceps is a harmful tapeworm and its larval form (coenurus cerebralis) is the causative agent of coenurosis, a disease affecting the health of herbivores, resulting in great economic loss to animal husbandry. Heat-shock proteins (HSPs), expressed in all prokaryotes and eukaryotes, act as molecular chaperones and can affect pathogenicity.MethodsHerein, cDNAs of T. multiceps genes Tm-HSP60 and Tm-p36 were cloned and molecularly characterised by bioinformatics analyses. The immunogenicity and immunoreactivity of recombinant rTm-HSP60 and rTm-p36 proteins were investigated by immunoblotting and indirect ELISA was established to evaluate their serodiagnostic potential. Tissue localisation and transcriptional level at different life stages of T. multiceps were determined by immunohistochemical and quantitative real-time PCR analyses.ResultThe 533 residue rTm-HSP60 and the 314 residue rTm-p36 proteins share typical highly conserved features of HSPs. Tm-p36 shares structural characteristics with metazoan small HSPs, with two N-terminal α-crystallin domains. Compared with Tm-p36, Tm-HSP60 displayed stronger immunogenicity, and the indirect ELISA based on rTm-HSP60 exhibited a sensitivity of 83.3% and a specificity of 87.5%, while rTm-p36 was not suitable to develop indirect ELISA. Tm-HSP60 was widely distributed in all stages of T. multiceps, albeit at relatively low levels, while Tm-p36 was specifically distributed in the protoscolex and oncosphere.ConclusionsThe sequence, structural and functional analyses of these two HSPs indicates that they may play important roles in the life-cycle of T. multiceps as molecular chaperones. Tm-HSP60 displayed stronger immunogenicity compare to Tm-p36, and has the potential for antibody detection. Tm-p36 was strongly associated with the activation of oncospheres and has potential interest for vaccination.Electronic supplementary materialThe online version of this article (10.1186/s13071-019-3352-8) contains supplementary material, which is available to authorized users.

show abstract

“…Intermediate hosts, such as goats, sheep, cattle and yaks, are infected with coenurosis following ingestion of pasture containing parasite eggs. Metacestodes of T. multiceps , known as Coenurus cerebralis , then parasitize the central nervous system and intermuscular, subcutaneous, and liver tissues of these intermediate hosts [ 4 , 5 , 6 ] and cause clinical manifestations such as circling symptoms, depression and even death [ 7 , 8 ], which lead to serious economic losses to farming, especially in economically developing countries within Africa and Southeast Asia [ 9 , 10 , 11 ]. Furthermore, this parasite also causes zoonotic infections in humans and poses severe threats to public health [ 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Annexin B2, B3 and B12 in Taenia multiceps

Guo

Xie

Liu

et al. 2018

Genes

Self Cite

View full text Add to dashboard Cite

Coenurus cerebralis, the metacestode of Taenia multiceps, causes coenurosis, a disease severely affecting goat, sheep, cattle and yak farming and resulting in huge economic losses annually. Annexins bind calcium ions and play an important role in flatworm parasite development. To explore potential functions of annexins in T. multiceps, three homologous genes, namely, TmAnxB2, TmAnxB3 and TmAnxB12, were screened from the transcriptome dataset, amplified from C. cerebralis cDNA and subjected to bioinformatics analysis. Then, polyclonal antibodies recognizing the recombinant TmAnxB2 (rTmAnxB2) and rTmAnxB3 were prepared for localization of TmAnxB2 and TmAnxB3 in different tissues and developmental stages by immunofluorescence. The transcription of all three genes was also measured by relative fluorescent quantitative PCR. The sizes of rTmAnxB2, rTmAnxB3 and rTmAnxB12 were 58.00, 53.06 and 53.51 kDa, respectively, and rTmAnxB12 was unstable. Both rTmAnxB2 and rTmAnxB3 were recognized by goat-positive T. multiceps sera in Western blots. Immunofluorescence revealed that TmAnxB2 and TmAnxB3 were localized in the protoscolex and cyst wall and TmAnxB3 was also detected in adult cortex. TmAnxB2 and TmAnxB12 mRNA levels were determined to be highest in oncospheres and protoscolex, whereas transcription of TmAnxB3 was highest in scolex and immature segments. Taken together, these findings indicate that TmAnxB2 and TmAnxB12 may play critical roles in T. multiceps larvae, while TmAnxB3 may have important functions in adults. These results will lay the foundation for functional research of annexins in T. multiceps.

show abstract

Molecular cloing and bioinformatics analysis of lactate dehydrogenase from Taenia multiceps

Cited by 6 publications

References 30 publications

Protoscolex metabolites of Coenurus cerebralis as antigenic-produced humoral immune response in sheep

Protoscolex metabolites of Coenurus cerebralis as antigenic-produced humoral immune response in sheep

Molecular characterisation and expression analysis of two heat-shock proteins in Taenia multiceps

Molecular Characterization of Annexin B2, B3 and B12 in Taenia multiceps

Contact Info

Product

Resources

About