Molecular characterization of the two postharvest biological control agents Aureobasidium pullulans L1 and L8

Francesco, Alessandra Di; Calassanzio, Matteo; Ratti, Claudio; Mari, Marta; Folchi, A.; Baraldi, Elena

doi:10.1016/j.biocontrol.2018.05.005

Cited by 26 publications

(25 citation statements)

References 24 publications

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“…However, A. melanogenum was recognized as an opportunistic human pathogen (Gostinčar et al 2014 ), so its use in biological control due to the potential health risk is considered inappropriate. Importantly, given that this species was only recently separated from A. pullulans , a phylogenetic analysis of the twenty A. pullulans strains used so far in biocontrol confirmed that only A. pullulans strains (Zajc et al 2020 ) were used in extensive postharvest disease control studies (Di Francesco et al 2018 ; Parafati et al 2015 ).…”

Section: Discussionmentioning

confidence: 94%

“…Gostinčar et al ( 2014 ) elevated the varieties of the A. pullulans species to the level of separate and well defined species based on large differences in genomic data together with distinctive ecology and physiology: A. pullulans, A. subglaciale, A. namibiae, and A. melanogenum . As A. melanogenum is recognized as opportunistic human pathogen (Gostinčar et al 2019 ), it should not be used as a biocontrol agent, while A. pullulans is one of the most extensively studied and used biocontrol agents, in particular for postharvest diseases (Di Francesco et al 2018 , 2020a ; Zhang et al 2010 ). Aureobasidium subglaciale, isolated so far only in some specialized environments and characterised by low temperatures and oligotrophic conditions, was recently proposed to be used in biocontrol of storage fungal diseases (Di Francesco et al 2020b ).…”

Section: Discussionmentioning

confidence: 99%

“…While VOCs production and bioactivity of A. pullulans have been studied in the past, no such studies were performed for A. subglaciale or even A. melanogenum. Also, the strains of A. pullulans studied and characterized for the biological control against crop diseases, were isolated from plants habitats such as fruit or leaf surfaces (Di Francesco et al 2018 ; Mounir et al 2007 ). Conversely, the present study considered the possible antagonistic activity of A. subglaciale and A. melanogenum strains isolated from non-plant related environment.…”

Section: Discussionmentioning

confidence: 99%

“…Whit regard to A. namibiae , the species name was based on a single isolate from Namib Desert marble (Zalar et al 2008 ), an extreme environment that could represent an interesting source for new microorganisms characterized by interesting and new antimicrobial metabolites. On the other hand, the species A. pullulans is recognized as one of the most promising biocontrol agents used in plant protection, in particular against postharvest diseases (Di Francesco et al 2018 ; Di Francesco et al 2020a ; Zhang et al 2010 ), while A. subglaciale is the least studied species in the field of crop defense, without any recognized biocontrol potential.…”

Section: Introductionmentioning

confidence: 99%

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Bioactivity of volatile organic compounds by Aureobasidium species against gray mold of tomato and table grape

Francesco

Zajc

Gunde‐Cimerman

et al. 2020

World J Microbiol Biotechnol

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Aureobasidium strains isolated from diverse unconventional environments belonging to the species A. pullulans, A. melanogenum, and A. subglaciale were evaluated for Volatile Organic Compounds (VOCs) production as a part of their modes of action against Botrytis cinerea of tomato and table grape. By in vitro assay, VOCs generated by the antagonists belonging to the species A. subglaciale showed the highest inhibition percentage of the pathogen mycelial growth (65.4%). In vivo tests were conducted with tomatoes and grapes artificially inoculated with B. cinerea conidial suspension, and exposed to VOCs emitted by the most efficient antagonists of each species (AP1, AM10, AS14) showing that VOCs of AP1 (A. pullulans) reduced the incidence by 67%, partially confirmed by the in vitro results. Conversely, on table grape, VOCs produced by all the strains did not control the fungal incidence but were only reducing the infection severity (< 44.4% by A. pullulans; < 30.5% by A. melanogenum, and A. subglaciale). Solid-phase microextraction (SPME) and subsequent gas chromatography coupled to mass spectrometry identified ethanol, 3-methyl-1-butanol, 2-methyl-1-propanol as the most produced VOCs. However, there were differences in the amounts of produced VOCs as well as in their repertoire. The EC50 values of VOCs for reduction of mycelial growth of B. cinerea uncovered 3-methyl-1-butanol as the most effective compound. The study demonstrated that the production and the efficacy of VOCs by Aureobasidium could be directly related to the specific species and pathosystem and uncovers new possibilities for searching more efficient VOCs producing strains in unconventional habitats other than plants.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bioactivity of volatile organic compounds by Aureobasidium species against gray mold of tomato and table grape

Francesco

Zajc

Gunde‐Cimerman

et al. 2020

World J Microbiol Biotechnol

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show abstract

“…The yeast like fungus Aureobasidium pullulans (De Bary) G. Armaud is a candidate biocontrol agent (BCA) and is naturally present in the phyllosphere and carposphere of several fruits and vegetables, and accompanied with the endophyte population of various species of plant (Bozoudi and Tsaltas 2018). A. pullulans has potential to control fruit and vegetable diseases caused by fungal pathogens primarily during the postharvest phase and can be used either alone or in combination with other sustainable physical methods (Di Francesco et al 2018;Di Francesco et al 2020;Di Francesco and Mari 2014;Zhang et al 2010). Certain A. pullulans strains have shown the ability to control fungal pathogens involved in causing post-harvest diseases, such as B. cinerea in grapes, Monilinia spp.…”

Section: Introductionmentioning

confidence: 99%

Biological control of strawberry crown rot, root rot and grey mould by the beneficial fungus Aureobasidium pullulans

Iqbal

Jamshaid

Zahid

et al. 2021

Preprint

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Utilization of biocontrol agents is a sustainable approach to reduce plant diseases caused by fungal pathogens. In the present study, we tested the effect of the candidate biocontrol fungus Aureobasidium pullulans (De Bary) G. Armaud on strawberry under in vitro and in vivo conditions to control, crown rot, root rot and grey mould caused by Phytophthora cactorum (Lebert and Cohn) and Botrytis cinerea Pers respectively. A dual plate confrontation assay showed that mycelial growth of P. cactorum and B. cinerea was reduced by 33- 48% when challenged by A. pullulans as compared with control treatments. Likewise, detached leaf and fruit assays showed that A. pullulans significantly reduced necrotic lesion size on leaves and disease severity on fruits caused by P. cactorum and B. cinerea . In addition, greenhouse experiments with whole plants revealed enhanced biocontrol efficacy against root rot and grey mould when treated with A. pullulans either in combination with the pathogen or pre-treated with A. pullulans followed by inoculation of the pathogens. Our results demonstrate that A. pullulans is an effective biocontrol agent to control strawberry diseases caused by fungal pathogens and can be an effective alternative to chemical-based fungicides.

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SCAR marker: A potential tool for authentication of agriculturally important microorganisms

Ambreetha

Balachandar

2022

J Basic Microbiol

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Microbial inoculants are globally recommended for plant growth promotion and control of plant pathogens. These inoculants require stringent quality checks for sustainable field efficacy. Questionable regulatory frameworks constantly deteriorate the reliability of bio‐inoculant technology. Existing global regulations do not involve any rapid molecular technique for the routine inspection of microbial preparations. Sequence characterized amplified region (SCAR) marker offers rapid and precise strain‐level authentication of target microbes. Such advanced molecular techniques must be exploited to accurately validate the microbial formulations. Besides, the global dissemination of plant pathogenic microbes has always been an alarming threat to food security. SCAR markers could be used at the plant quarantine centers to rapidly detect catastrophic pathogens, thereby circumventing the import and export of contagious plant materials. The current review is focused on promoting the SCAR marker technology to validate commercial bio‐inoculants and predict plant pandemics.

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Molecular characterization of the two postharvest biological control agents Aureobasidium pullulans L1 and L8

Cited by 26 publications

References 24 publications

Bioactivity of volatile organic compounds by Aureobasidium species against gray mold of tomato and table grape

Bioactivity of volatile organic compounds by Aureobasidium species against gray mold of tomato and table grape

Biological control of strawberry crown rot, root rot and grey mould by the beneficial fungus Aureobasidium pullulans

SCAR marker: A potential tool for authentication of agriculturally important microorganisms

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