Molecular characterization of some brinjal genotypes (Solanum melongena L) using simple sequence repeat (SSR) markers

Abstract

To analyze genetic variability of 48 brinjal genotypes by Simple Sequence Repeat (SSR) markers, four primers named smSSR01, smSSR03, smSSR11 and smSSR14 were selected for analyze the data. Four primers generated clear bands and a total of 44 alleles were detected among the 48 brinjal genotypes (9.0 to 14.0 alleles per locus with a mean of 11.0 alleles per locus). According to Nei's (1973), the highest level of gene diversity value 0.8672 was observed in loci smSSR11 and the lowest level of gene diversity value 0.6580 was observed in the loci smSSR14 with a mean diversity of 0.7847. As a measure of the informativeness of microsatellites, the PIC value ranges from a low of 0.6413 (smSSR14) to a high of 0.8536 (smSSR11) and averaged 0.7660. The genetic distance (GD) between genotypes was computed from combined data for the four primers, ranging from 0.250 to 1.000. The highest genetic distance (1.000) was observed between Morich Begun (Small) vs. Laffa (Long, Violet), Morich Begun (Small) vs. BARI Begun-1 and many other genotype pairs and also the lowest genetic distance (0.250) was found in Morich Begun (Small) vs. Pahoza-2, Morich Begun (Small) vs. BAU Begun-1, Singnath (Long, Violet) vs. Laffa BAU (Long, Violet), Borka (Long, Green) vs. Purple Long and many other genotype pairs. The Unweighed Pair Group Method with Arithmetic Means (UPGMA) cluster tree analysis lead to the grouping of the 48 genotypes into seven major clusters and the genotypes that are derivatives of genetically similar type form cluster together. Maximum brinjal genotypes include cluster IV. The SSR markers used in the present study were able to differentiate forty eight brinjal genotypes genetically. The results of the study suggested that SSR can be used as a suitable genetic marker to identify the brinjal genotypes. However, further studies involving large number of genotypes and primers need to be conducted to get more precise information and help enhance the knowledge of students and researchers relating to molecular characterization of other solanaceious crops.

Genetic diversity of exotic and local eggplants (Solanum spp.) cultivated in Côte d’Ivoire based on ISSR markers

“…The brinjal eggplants, S. melongena, are derived from the wild African species S. incanum L. and was domesticated in India and southeast China (Taher et al 2017). Now, brinjal eggplant is cultivated throughout the world (Ahmed et al 2019).…”

“…However, a comprehensive understanding of the molecular variation and the knowledge about the genetic relationships among these eggplant genotypes are needed for not only breeding purposes, but also for the implementation of potential genetic conservation programs (Ahmed et al 2019).…”

Microsatellite marker assisted molecular and morpho-physiological genetic diversity assessment in 38 genotypes of sesame (Sesamum indicum L.)

“…To evaluate the genetic diversity of different sesame genotypes the means of genetic distances between genotypes were used and it also revealed wide variability among the tested genotypes. Using SSR marker, Ahmed et al (2019) recorded genetic distance between 48 genotypes of brinjal genotypes ranging from 0.250 to 1.00 indicating good capacity of SSR markers to clearly discriminate among plants of near or distant genetic backgrounds. The information on microsatellite markers collected in our research was also capable to analyze diversity among all the genotypes through cluster analysis.…”