Molecular characterization of olive (Olea europaea L.) Sicilian cultivars using SSR markers

Casas, Giuseppina Las; Scollo, Francesco; Distefano, Gaetano; Continella, Alberto; Gentile, Alessandra; Malfa, Stefano La

doi:10.1016/j.bse.2014.07.010

Cited by 20 publications

(22 citation statements)

References 21 publications

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“…Statistically, a total of 110 marker alleles over ten SSR genomic loci utilized in this study showed an average number of observed alleles per locus Na = 9. This number is comparable with the 135 marker alleles with an average of 9.6 per locus published by Lopes et al (2004), and 67 marker alleles with an average 8.4 found among Sicilian accessions reported by Las Casas et al (2014). Similarly, 75 marker alleles with an average of 6.8 were reported for olive cultivars in Southern Italy by Muzzalupo et al (2009), and higher than 104 marker alleles with average 3.6 reported by Cipriani et al (2002).…”

Section: First Evidences On the Origin And Spreading Of Veneto Olivessupporting

confidence: 79%

Venetian olive (Olea europaea) germplasm: disclosing the genetic identity of locally grown cultivars suited for typical extra virgin oil productions

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Mariotti

Ruperti

et al. 2018

Genet Resour Crop Evol

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Olive (Olea europaea L.) is one of the most important tree crops of the Mediterranean regions. In spite of the increasing appreciation of typical extra virgin olive oils at world level, based on the use of local traditional varieties, very few studies have focused on the genetic characterisation of olive cultivars of regional interest, such as those grown in Veneto, a NorthEastern Italy region. A deep knowledge of the varieties cultivated in this territory is a key step to address the product quality, to increase market demand and to certify the origin of local olive oils. Here we have analyzed olive cultivars and cultivar groups within the olive cultivation area in Veneto, from the Garda Lake to the Euganean and Trevisan hills, by using discriminant SSR markers, in order to obtain a systematic genetic survey of the Veneto regional olive germplasm patrimony. A total of 203 previously uncharacterized olive samples were collected from ancient trees still grown by local farmers. The analyzed samples included also 36 olive reference cultivars from Veneto and neighbour Regions. We found 57 unique molecular profiles out of this set of olive accessions that were split into 15 cultivar groups corresponding to genetically distinct STRUCTURE clusters. Based on a common SSR database, our 239 Venetian accessions were compared with 280 olive reference genotypes representative of the Mediterranean cultivation area. From the genetic structure analysis, it has been observed that 80% of Venetian cultivars clustered in the central Mediterranean group, about 9% and 2% with the eastern and western varieties, respectively, and all the others resulted intermixed among two or three populations. We found that regionally the most common variety was ''Casaliva'', corresponding to the widely diffused cultivar ''Frantoio'', while others showed identity with known varieties grown in close regions, such as ''Leccino'', ''Miniol'', ''Capolga'' and ''Bianchera''. Besides these genotypes, others were not matching any known reference and therefore they could be classified as true local varieties of indigenous origin, possibly deriving from the hybridization and selection made by

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Section: First Evidences On the Origin And Spreading Of Veneto Olivessupporting

confidence: 79%

Venetian olive (Olea europaea) germplasm: disclosing the genetic identity of locally grown cultivars suited for typical extra virgin oil productions

Hmmam

Mariotti

Ruperti

et al. 2018

Genet Resour Crop Evol

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“…French olive growing is still mainly founded on a traditional system involving a diverse range of crops and varieties (Pinatel, 2015). This could be viewed as a key factor favoring varietal diversity, as previously noted by several authors (Gemas et al, 2004;Khadari et al, 2008;Kaya et al, 2013;Marra et al, 2013;Las Casas et al, 2014;Xanthopoulou et al, 2014). Here, we assumed that the French varietal diversity could mainly be explained by active farmer selection, probably due to the impact of relatively frequent climatic accidents on local germplasm.…”

Section: French Olive Agroecosystems As Varietal Diversification Incumentioning

confidence: 53%

Cultivated Olive Diversification at Local and Regional Scales: Evidence From the Genetic Characterization of French Genetic Resources

et al. 2019

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Molecular characterization of crop genetic resources is a powerful approach to elucidate the origin of varieties and facilitate local cultivar management. Here we aimed to decipher the origin and diversification of French local olive germplasm. The 113 olive accessions of the ex situ collection of Porquerolles were characterized with 20 nuclear microsatellites plus their plastid haplotype. We then compared this collection to Mediterranean olive varieties from the Worldwide Olive Germplasm Bank of Marrakech, Morocco. High genetic diversity was observed within local French varieties, indicating a high admixture level, with an almost equal contribution from the three main Mediterranean gene pools. Nearly identical and closely related genotypes were observed among French and Italian/Spanish varieties. A high number of parent-offspring relationships were also detected among French varieties and between French and two Italian varieties ('Frantoio' and 'Moraiolo') and the Spanish variety ('Gordal Sevillana'). Our investigations indicated that French olive germplasm resulted from the diffusion of material from multiple origins followed by diversification based on parentage relationships between varieties. We strongly suggest that farmers have been actively selecting olives based on local French varieties. French olive agroecosystems more affected by unexpected frosts than southernmost regions could also be seen as incubators and as a bridge between Italy and Spain that has enhanced varietal olive diversification.

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“…However, the moderate levels of genetic diversity but low degrees of genetic differentiation of the tested germplasms may be because of the limited number of SSR primers, not evenly distributed throughout the genome. More SSR primers or other markers, such as single-nucleotide polymorphisms (SNPs), may be applicable (Las et al, 2014) for effective genetic characterization and population structure analysis of Chinese fir. Genetic variations in red-colored heartwood Chinese fir have been analyzed in a variety of species (Zhang, 2009); however, further experiments are required to study Chinese fir genetic diversity.…”

Section: Discussionmentioning

confidence: 99%

Genetic characterization of red-colored heartwood genotypes of Chinese fir using simple sequence repeat (SSR) markers

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The present study investigated the genetic characterization of red-colored heartwood Chinese fir [Cunninghamia lanceolata (Lamb.) Hook.] in Guangxi using 21 simple sequence repeat (SSR) markers and analyzes of the genetic variation (N = 149) in samples obtained from five sites in Guangxi Province, China. The number of different alleles and the Shannon's information index per locus ranged from 3 to 12 and from 0.398 to 2.258 with average values of 6 and 1.211, respectively, indicating moderate levels of genetic diversity within this germplasm collection. The observed and expected heterozygosity ranged from 0.199 to 0.827 and from 0.198 to 0.878 with an average of 0.562 and 0.584, respectively. Although, the mean fixation index was 0.044, indicative of a low level of genetic differentiation among germplasms, analysis of molecular variance revealed considerable differentiation (99%) within the (2015) samples. The neighbor-joining dendrogram revealed that the majority of red-colored Chinese fir genotypes were apparently not associated with their geographic origins. Further analysis by STRUCTURE showed that this Guangxi germplasm collection could be divided into three genetic groups comprising 76, 37, and 36 members, respectively; these were classified into mixed groups with no obvious population structure. These results were consistent with those of the cluster analysis. On the whole, our data provide a starting point for the management and conservation of the current Guangxi germplasm collection as well as for their efficient use in Chinese fir-breeding programs.

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Molecular characterization of olive (Olea europaea L.) Sicilian cultivars using SSR markers

Cited by 20 publications

References 21 publications

Venetian olive (Olea europaea) germplasm: disclosing the genetic identity of locally grown cultivars suited for typical extra virgin oil productions

Venetian olive (Olea europaea) germplasm: disclosing the genetic identity of locally grown cultivars suited for typical extra virgin oil productions

Cultivated Olive Diversification at Local and Regional Scales: Evidence From the Genetic Characterization of French Genetic Resources

Genetic characterization of red-colored heartwood genotypes of Chinese fir using simple sequence repeat (SSR) markers

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