2022
DOI: 10.1080/23766808.2022.2080334
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Molecular characterization of mahogany tree (Swietenia macrophylla King, Meliaceae) in the remnant natural forest of Ecuador

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Cited by 2 publications
(3 citation statements)
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“…The effective size of the variance Ne is important to be able to determine the minimum number of seed donor trees in the collection process. Thus, the results show the importance of estimating the size of the samples designated for use in breeding programs, genetic conservation, seed collection, and addressing environmental recovery, as well as for the monitoring of genetic diversity in manipulated populations [24,42,44].…”
Section: Crossing Systemmentioning
confidence: 97%
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“…The effective size of the variance Ne is important to be able to determine the minimum number of seed donor trees in the collection process. Thus, the results show the importance of estimating the size of the samples designated for use in breeding programs, genetic conservation, seed collection, and addressing environmental recovery, as well as for the monitoring of genetic diversity in manipulated populations [24,42,44].…”
Section: Crossing Systemmentioning
confidence: 97%
“…These microsatellite loci were amplified by polymerase chain reaction (PCR) using a Veriti thermal cycler (Applied Biosystems, Foster City, CA, USA) with a total volume of 10 µL per reaction (containing 10 ng of genomic DNA, 1× buffer, 210 µM of each dNTP, 1.5 mM MgCl 2 , 0.16 µM forward primer and M13 (FAM or NED) broth [23], 0.32 µM reverse primer, 1.05 U Taq DNA polymerase (Invitrogen, Carlsbad, CA, USA), and 3.49 µL ultrapure water. These PCR amplifications consisted of two steps, of which the first was primer-specific and the second M13 binding [24]. The amplification products were checked by electrophoresis on 1.5% agarose gels stained with GelRed (Biotium, Fremont, CA, USA) in 1× TBE buffer (pH 8.0).…”
Section: Amplification Of Microsatellite Markersmentioning
confidence: 99%
“…Swietenia macropnylla King (SMK) belongs to the family Meliaceae, which is commonly known as mahogany. It is native to the tropical regions of America, spreading from Materials 2023, 16, 6558 2 of 11 southern Mexico to the North of Brazil [11]. In Taiwan, SMK has been extensively planted in the plain area to exploit it as available wood material for making furniture and other advanced wood products.…”
Section: Introductionmentioning
confidence: 99%