Molecular characterization of G1 human rotaviruses detected in children from Belém, Pará, Brazil

Abstract

Caracterização molecular de rotavírus humanos do tipo G1 detectados em crianças de Belém, Pará, Brasil Caracterización molecular de rotavirus humano tipo G1 detectado en niños de Belém (Estado de Pará, Brasil)

Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia

“…Ten G types and 11 P types (mostly, G1-G6, G8-G10, and G12, and P [4], P [6], P [8], and P [9], respectively) have been recovered in association with human infections. The combinations G1P [8], G2P [4], G3P [8], and G9P [8] are the most frequently found [3][4] .…”

“…Ten G types and 11 P types (mostly, G1-G6, G8-G10, and G12, and P [4], P [6], P [8], and P [9], respectively) have been recovered in association with human infections. The combinations G1P [8], G2P [4], G3P [8], and G9P [8] are the most frequently found [3][4] . Recently, a study on the etiology of gastroenteritis in the infantile population showed group A rotavirus to be the most frequent enteropathogen, occurring in 23.6% of the total diarrhea cases; the second group consisted entirely of pathogenic categories of diarrheogenic Escherichia coli differentiated by PCR technique, occurring in 18.2% of cases, followed by Salmonella sp.…”

“…For P genotypes, the first PCR was performed using the consensus primer pair con2/con3; one fragment of 876bp was amplified. In the nested reaction, the combinations were between con3 primer and the pool of P-specific primers for variable regions of the VP4 gene, 1T-1, 2T-1, 3T-1, and 4T-1, specifically to the P [8], P [4], P [6], and P[9] genotypes 9 . The products were visualized under UV in 1% agarose gels stained with ethidium bromide.…”

“…When the G and P genotyping results were combined, the most frequent genotypes observed were identified as G1P [8], G2P [4], G3P [8], and G4P [8] (Table 1), the G1P [8] genotype being the most common, found in 61% of samples; this was followed by G2P [4] in 20%, G3P [8] in 6.7%, and G4P [8] in 3.3%. Some samples with G1 and G2 genotypes did not have had their P genotype identified; these represented 3.3% and 5% of the samples, respectively.…”

Diversidade molecular de rotavírus do grupo A na cidade de Manaus, Estado do Amazonas, Brasil, 2004 a 2006

“…23 Outros genótipos G que podem ser citados neste estudo são o G8 e o G10, que embora considerados atípicos, já foram relatados em outro estudo. 26 Outros genótipos G e P que podem ser citados são o G11, o G12 e o P [25], identiicados em estudo realizado no Nepal, onde, na caracterização molecular do RVA em amostras de fezes de crianças e adultos, foram encontrados os genótipos G11 e G12 e o genótipo P [25], este último na forma da combinação P [25]G11. 27 Os dados encontrados neste estudo demonstram que genótipos anteriormente tidos como restritos a animais, que ocorrem em humanos como resultado de eventos de transmissão zoonótica -a exemplo do G5 encontrado em suínos 28 ou do G6, do G8 e do G10 isolados inicialmente em bovinos 29 -, podem estar sendo transmitidos de animais para humanos, seja como vírus inteiros ou como reorganização de segmentos de genes.…”