Molecular Characterization of Fusarium Solani Degrades a Mixture of Low and High Molecular Weight Polycyclic Aromatic Hydrocarbons

Hesham, Abd El-Latif; Elsayed, Mohamed; Mawad, Asmaa M. M.; Elfarash, Ameer; El-Fattah, Bahaa S. Abd; El-Rawy, Mahmoud A.

doi:10.2174/1874070701711010027

Cited by 15 publications

(12 citation statements)

References 37 publications

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“…Regarding fungi, this could be explained by their physiological growing capabilities. Thanks to their hyphae growth, they can widely colonize the soil, creating a homogenous mycoflora in the space ( Hesham et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…The isolation of 42 strains of Fusarium , among which 64% were ascribable to F. solani , opens to intriguing solutions for their application in bioremediation processes. Indeed F. solani isolated from oil polluted soil by enrichment method using phenanthrene as the sole source of carbon was capable of degrading a mixture of low and high molecular weight PAHs (up to 84.8%) ( Hesham et al, 2017 ). To the best of our knowledge, this is the first time that a strain of Eutypella scoparia has been isolated from a polluted soil.…”

Section: Discussionmentioning

confidence: 99%

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Molecular and Microbiological Insights on the Enrichment Procedures for the Isolation of Petroleum Degrading Bacteria and Fungi

et al. 2018

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Autochthonous bioaugmentation, by exploiting the indigenous microorganisms of the contaminated environment to be treated, can represent a successful bioremediation strategy. In this perspective, we have assessed by molecular methods the evolution of bacterial and fungal communities during the selective enrichment on different pollutants of a soil strongly polluted by mixtures of aliphatic and polycyclic hydrocarbons. Three consecutive enrichments were carried out on soil samples from different soil depths (0–1, 1–2, 2–3 m), and analyzed at each step by means of high-throughput sequencing of bacterial and fungal amplicons biomarkers. At the end of the enrichments, bacterial and fungal contaminants degrading strains were isolated and identified in order to (i) compare the composition of enriched communities by culture-dependent and culture-independent molecular methods and to (ii) obtain a collection of hydrocarbon degrading microorganisms potentially exploitable for soil bioremediation. Molecular results highlighted that for both bacteria and fungi the pollutant had a partial shaping effect on the enriched communities, with paraffin creating distinct enriched bacterial community from oil, and polycyclic aromatic hydrocarbons generally overlapping; interestingly neither the soil depth or the enrichment step had significant effects on the composition of the final enriched communities. Molecular analyses well-agreed with culture-dependent analyses in terms of most abundant microbial genera. A total of 95 bacterial and 94 fungal strains were isolated after selective enrichment procedure on different pollutants. On the whole, isolated bacteria where manly ascribed to Pseudomonas genus followed by Sphingobacterium, Bacillus, Stenothrophomonas, Achromobacter, and Serratia. As for fungi, Fusarium was the most abundant genus followed by Trichoderma and Aspergillus. The species comprising more isolates, such as Pseudomonas putida, Achromobacter xylosoxidans and Ochromobactrum anthropi for bacteria, Fusarium oxysporum and Fusarium solani for fungi, were also the dominant OTUs assessed in Illumina.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Molecular and Microbiological Insights on the Enrichment Procedures for the Isolation of Petroleum Degrading Bacteria and Fungi

et al. 2018

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“…The use of 16SrRNA gene sequences for bacterial identifications is a more accurate method than traditional methods included in Bergey’s Manual of Systematic Bacteriology [ 18 - 21 ]. The nuclear ribosomal Internal Transcribed Spacer ITS region is widely used as a DNA meta-barcoding marker to identify fungal isolates [ 13 , 22 - 24 ].…”

Section: Discussionmentioning

confidence: 99%

Role of Bacterial-Fungal Consortium for Enhancement in the Degradation of Industrial Dyes

Mawad

Hesham

Yousef

et al. 2020

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Background: The presence of anthraquinone (Disperse blue 64) and azodyes (Acid yellow 17) in a waterbody are considered among the most dangerous pollutants. Methods: In this study, two different isolated microbes, bacterium and fungus, were individually and as a co-culture applied for degradation of Disperse Blue 64 (DB 64) and Acid Yellow 17 (AY 17) dyes. The isolates were genetically identified based upon 16S (for bacteria) and ITS/5.8S (for fungus) rRNA genes sequences as Pseudomoans aeruginosa and Aspergillus flavus respectively. Results: The fungal/bacterial consortium exhibited a higher percentage of dyes degradation than the individual strains even at high concentration of 300 mg/L. Azoreductase could be identified as the main catabolic enzyme and the consortium could induce azoreductase enzyme in the presence of both dyes. However, the specific substrate which achieved the highest azoreductase specific activity was Methyl red (MR) (3.5 U/mg protein). The tentatively proposed metabolites that were detected by HPLC/MS suggested that the reduction process catalyzed the degradation of dyes. The metabolites produced by the action consortium on two dyes were safe on Vicia faba and Triticum vulgaris germination and health of seedlings. Toxicity of the dyes and their degradation products on the plant was different according to the type and chemistry of these compounds as well as the type of irrigated seeds. Conclusion: We submit that the effective microbial degradation of DB64 and AY17 dyes will lead to safer metabolic products.

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“…However, no effective method for the removal of existing PAHs in the environment has yet been developed [ 4 , 5 ]. Most of the recent researches have attempted the biological degradation of PAHs by bacteria, fungi, yeasts and higher plants [ 6 - 8 ]. Bacterial degradation of PAHs is more preferable as it is fast, can be aerobical, anaerobical and under variable environmental conditions [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Quantification of Naphthalene Dioxygenase (NahAC) and Catechol Dioxygenase (C23O) Catabolic Genes Produced by Phenanthrene-Degrading Pseudomonas fluorescens AH-40

Mawad

Abdel-Mageed

Hesham

2020

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Background: Petroleum polycyclic aromatic hydrocarbons (PAHs) are known to be toxic and carcinogenic for humans and their contamination of soils and water is of great environmental concern. Identification of the key microorganisms that play a role in pollutant degradation processes is relevant to the development of optimal in situ bioremediation strategies. Objective: Detection of the ability of Pseudomonas fluorescens AH-40 to consume phenanthrene as a sole carbon source and determining the variation in the concentration of both nahAC and C23O catabolic genes during 15 days of the incubation period. Methods: In the current study, a bacterial strain AH-40 was isolated from crude oil polluted soil by enrichment technique in mineral basal salts (MBS) medium supplemented with phenanthrene (PAH) as a sole carbon and energy source. The isolated strain was genetically identified based on 16S rDNA sequence analysis. The degradation of PAHs by this strain was confirmed by HPLC analysis. The detection and quantification of naphthalene dioxygenase (nahAc) and catechol 2,3-dioxygenase (C23O) genes, which play a critical role during the mineralization of PAHs in the liquid bacterial culture were achieved by quantitative PCR. Results: Strain AH-40 was identified as pseudomonas fluorescens. It degraded 97% of 150 mg phenanthrene L-1 within 15 days, which is faster than previously reported pure cultures. The copy numbers of chromosomal encoding catabolic genes nahAc and C23O increased during the process of phenanthrene degradation. Conclusion: nahAc and C23O genes are the main marker genes for phenanthrene degradation by strain AH-40. P. fluorescence AH-40 could be recommended for bioremediation of phenanthrene contaminated site.

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Molecular Characterization of Fusarium Solani Degrades a Mixture of Low and High Molecular Weight Polycyclic Aromatic Hydrocarbons

Cited by 15 publications

References 37 publications

Molecular and Microbiological Insights on the Enrichment Procedures for the Isolation of Petroleum Degrading Bacteria and Fungi

Molecular and Microbiological Insights on the Enrichment Procedures for the Isolation of Petroleum Degrading Bacteria and Fungi

Role of Bacterial-Fungal Consortium for Enhancement in the Degradation of Industrial Dyes

Quantification of Naphthalene Dioxygenase (NahAC) and Catechol Dioxygenase (C23O) Catabolic Genes Produced by Phenanthrene-Degrading Pseudomonas fluorescens AH-40

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