Molecular Characterization of Corynebacterium bovis causing Clinical Mastitis and Increasing Somatic-Cell Count

Langoni, Hélio; Guimarães, F. F.; Salina, A.; Ribeiro, Márcio Garcia; P.V.P., Baio; Ramos, Juliana Nunes; Mota, Higor Franceschi; Vieira, Verônica Viana; Mattos-Guaraldi, A L

doi:10.23953/cloud.ijavst.172

Cited by 3 publications

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References 11 publications

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“…On the other hand, the H-SCC group tended to have more Corynebacterium spp. Although there are conflicting opinions on the relevance of the commensal pathogen Corynebacterium bovis as an etiological agent of mastitis [ 1 ], the general consensus is that it is an important causative agent of mastitis [ 22 ]. Coryneforms do not reach udder tissues, but seem to colonize only the teat canal [ 2 ].…”

Section: Discussionmentioning

confidence: 99%

Examination of the microbiota of normal cow milk using MinION<sup>TM</sup> nanopore sequencing

Shinozuka

Kawai

Kurumisawa

et al. 2021

The Journal of Veterinary Medical Science

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The aim of this study was to evaluate the microbiota of normal milk in dairy cows and their relationship with host factors, such as the age of the cow (Age), somatic cell counts in milk (SCCs), and days in milk (DIM). We investigated 48 milk samples from 22 cows with no systemic or local clinical signs using MinION TM nanopore sequencing for a 16S rRNA gene amplicon. Bacterial richness was positively correlated with the DIM (p = 0.043), and both the Shannon-Wiener Index and Simpson's Index, which are metrics of alpha-diversity, were also significantly positively correlated with the SCC (p < 0.001). The composition ratios of both Actinobacteria at the phylum level and Kocuria spp. at the genus level in the milk microbiota were significantly correlated with the SCC (p < 0.001 and p < 0.001, respectively). In the beta-diversity test, the one-way analysis of similarities test showed a significant difference (p = 0.0051) between the low-and high-SCC groups. This study clarified that the composition of the normal milk microbiota in this herd was related to the SCC. It also raised the possibility of variations in bacterial genera in the normal milk microbiota between the low-and high-SCC groups.However, to clarify the actual condition of the milk microbiota and to elucidate the relationship with the SCC, it is necessary to perform further analyses taking into account not only the relative abundance, but also the absolute abundance of microbes.

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Section: Discussionmentioning

confidence: 99%

Examination of the microbiota of normal cow milk using MinION<sup>TM</sup> nanopore sequencing

Shinozuka

Kawai

Kurumisawa

et al. 2021

The Journal of Veterinary Medical Science

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Considerações sobre o tratamento das mastites

et al. 2017

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RESUMO: A mastite é considerada o maior problema dos animais destinados à produção de leite. Altera a sua composição e aumenta a contagem de células somáticas (CCS). Os micro-organismos envolvidos na doença podem ser origem infecciosa, como Staphylococcus aureus, ou ambiental, tal como Escherichia coli. A cultura bacteriana é uma ferramenta de diagnóstico e auxilia na detecção do patógeno causador da mastite. No entanto, fatores como fagocitose podem desencadear um resultado negativo. Quando estabelecido um programa de controle de mastite, o diagnóstico precoce e o início do tratamento adequado dos casos clínicos são fundamentais para se atingir os objetivos e seu sucesso, está relacionado com o patógeno envolvido. A indicação do tratamento de longa duração, ou terapia estendida, tem melhorado a resposta ao tratamento em casos de mastite por S. aureus, no entanto, com 30-50% de cura. Do ponto de vista do manejo dos animais, devido a alta contagiosidade deste patógeno, sua persistência no rebanho e custo em função ao tratamento, muitas vezes, o descarte do animal tem sido priorizado a fim de controlar os casos de mastite em propriedades. As medidas de controle são muito importantes para contribuir com a redução de casos de mastite por este patógeno. A indicação do tratamento intramamário associada com sistêmico tem poder efetivo em casos de mastite por E. coli, cujos casos agudos apresentam-se com sepse e toxemia. São abordados ainda aspectos de tratamentos alternativos das mastites, utilizados principalmente no processo orgânico de produção leiteira.

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Short communication: Identification of Corynebacterium bovis by MALDI-mass spectrometry

Langoni

Silva

Troncarelli

et al. 2017

Journal of Dairy Science

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Corynebacterium bovis is a mastitis-causing microorganism responsible for economic losses related to decrease in milk production. The aim of the study was identify Corynebacterium spp. strains recovered from milk samples of subclinical mastitis by using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Samples were collected during a 10-mo mastitis-monitoring program in a high-production dairy farm. In this study, 80 strains were analyzed; from these 54 (67.5%) were identified at species level as Corynebacterium bovis, 24 (31.2%) isolates were identified at the genus level as Corynebacterium spp., and only 1 (1.35%) isolated had unreliable identification. Results demonstrated that MALDI-MS could be an important technique for the identification of Corynebacterium spp. in milk.

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Molecular Characterization of Corynebacterium bovis causing Clinical Mastitis and Increasing Somatic-Cell Count

Cited by 3 publications

References 11 publications

Examination of the microbiota of normal cow milk using MinION<sup>TM</sup> nanopore sequencing

Examination of the microbiota of normal cow milk using MinION<sup>TM</sup> nanopore sequencing

Considerações sobre o tratamento das mastites

Short communication: Identification of Corynebacterium bovis by MALDI-mass spectrometry

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