Molecular characterization of bot flies, Oestrus spp., (Diptera, Oestridae), from domestic and wild Bovidae hosts

Moreno, Virginia; Romero-Fernández, Ismael; Marchal, Juan A.; Beltrán, Manuel; Granados, José E.; Habela, M.; Tamadon, Amin; Rakhshandehroo, Ehsan; Sarasa, Mathieu; Pérez, Jesús M.; Sánchez, Antonio

doi:10.1016/j.vetpar.2015.08.002

Cited by 20 publications

(10 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 658 bp region of CO1 is widely accepted as a universal and standard marker for all animal taxa (14). During the last decade, DNA barcoding using CO1 sequences has provided an efficient tool for molecular identification and phylogenetic characterization of several kinds of insect species including Oestridae family (16)(17)(18)(19). In this study, we aimed to characterize the O. ovis larvae from an infested human with naso-pharyngeal myiasis by using CO1 barcode sequences.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Phylogenetic Analyses of <i>Oestrus ovis</i> Larvae Causing Human Naso-pharyngeal Myiasis Based on CO1 Barcode Sequences

Karademir¹,

Usluğ²,

Okur³

et al. 2020

Turkiye Parazitol Derg

View full text Add to dashboard Cite

Amaç: Bu çalışmada, naso-pharyngeal myiasis ile enfekte bir insandan elde edilmiş bot sineği larvalarının identifikasyonu ve moleküler karakterizasyonu amaçlanmıştır. Yöntemler: Adana bölgesinde naso-pharyngeal myiasis enfeksiyonu olan 49 yaşındaki bir kadından elde edilen sekiz adet bot sineği larvası çalışmanın materyalini oluşturmuştur. Larvaların teşhis anahtarlarına göre morfolojik teşhisleri yapılmıştır. Larva örneklerinin genomik DNA ekstraktlarında barkod CO1 geni amplifiye edilmiştir. CO1 sekanslarında haplotip ve genetik farklılık analizleri yapılmış ve ilişkileri göstermek için filogenetik ağaç oluşturulmuştur. Bulgular: Morfolojik özelliklerine göre tüm bot sineği larvaları O. ovis'in ikinci dönem larvası olarak teşhis edilmiştir. İzolatların CO1 sekansları arasında polimorfizm belirlenmemiş olup bu sonuç O. ovis için yeni bir haplotipin varlığını ortaya çıkarmıştır. Çalışmada yeni karakterize edilen haplotip Bosna-Hersek, Hırvatistan, Brezilya ve İran'dan rapor edilmiş O. ovis haplotipleriyle ortalama %99,5 identiklik göstererek kümelenme göstermiştir. Oestridae alt aileleri arasındaki interspesifik genetik farklılıklar %19,8-%30,8 olarak belirlenmiştir. Sonuç: Bu çalışma Türkiye'de rastlantısal bir insan konakta belirlenen O. ovis larvaları üzerine CO1 barkod sekansları temelinde ilk moleküler karakterizasyon verilerini sağlamıştır.

show abstract

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Phylogenetic Analyses of <i>Oestrus ovis</i> Larvae Causing Human Naso-pharyngeal Myiasis Based on CO1 Barcode Sequences

Karademir¹,

Usluğ²,

Okur³

et al. 2020

Turkiye Parazitol Derg

View full text Add to dashboard Cite

show abstract

“…Oestrid species' molecular data is extremely scarce. Similarly, a study by (Moreno et al, 2015) found that O.…”

Section: Discussionmentioning

confidence: 79%

“…Data on Oestrid species is extremely limited. Also (Moreno et al, 2015) found that O. ovis was parasitizing all of the host species studied, except for Iberian ibex (which is probably the focus of a different Oestrus species). (Stevens , 2003) found a (98)% homology with accession number AJ551428.1 in Italy: Apulia.…”

Section: Discussionmentioning

confidence: 90%

Sequence analyzing of Oestrus ovis in sheeps in Al-Diwaniyah province

Al-Hameedawi

Jasim

2022

ijhs

View full text Add to dashboard Cite

A total of 364 head sheep were examine from fourteen region in Al- Diwaniyah province 224 positive to infestation and larvae were collected and sent to the laboratory of veterinary medicine, from October 2021 to March 2022 for both sexes. The rate of Oestrus ovis infestation in sheep in the study areas was 61.53%. Al-Dighara abattoir had the highest infestation rate (82.75%) and Al-Salahia had the lowest infestation rate (17.64%). DNA extraction sequencing and phylogenetic analysis were used to identify Oestrus ovis larvae (16S rRNA) gene, the sequence was recoded in the National Center Biotechnology (NCBI) with accession numbers (ON059972, ON059973, ON059974, ON059975, ON059976, ON059977, and ON059978) Iraq for O.ovis larvae. The percentage of homology sequence identity between this gene and another global gene registered in NCBI was (99–100%) with Brazil and Bosnia and Herzegovina isolates, (97.17%) with USA isolates, and (96.17, 96.33, and 96.68%) with Brazil, Bosnia and Herzegovina, and USA isolates, respectively.

show abstract

“…Besides their hosts of predilection, O. ovis has been reported in wild animals as Ovis canadensis (CAPELLE, 1966), Lama glama (GOMEZ-PUERTA et al, 2013), Ovis orientalis musimon (MORENO et al, 1999(MORENO et al, , 2015, Capra sibirica (SÁNCHEZ et al, 2017) and Ammotragus lervia (BARROSO et al, 2017). This larvae is also common cause of ophthalmomyiasis in humans (FASIH et al, 2014; SANTE FERNÁNDEZ et al, 2017; RAO et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Immune humoral response of young lambs naturally infested by Oestrus ovis (Diptera: Oestridae)

Silva

Bassetto

Amarante

2018

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

Twenty-six newborn lambs were evaluated for 21 weeks, from birth to slaughter, to assess their plasma anti-Oestrus ovis immunoglobulin (IgG) using the ELISA technique. On the last day of sampling, all the lambs were slaughtered and O. ovis larvae were recovered, quantified and identified according to the larval stage. High levels of IgG were observed over the first three weeks of life, thus indicating that antibodies are transferred via colostrum from ewes to lambs. Afterwards, the antibody levels declined progressively until the lambs were 11 weeks of age and subsequently started to increase again when they were around 13 weeks of age, reaching the apex on the last week of sampling. All the lambs were parasitized with different larval stages of O. ovis, with an average of 39 larvae per lamb, and the intensity of the infestation ranged from 10 to 97 larvae. However, there was non-significant correlation coefficients between IgG levels and O. ovis larval burden (P > 0.05). In conclusion, although the lambs became infested with O. ovis at an early age, the larval burden was not associated with specific IgG levels.

show abstract

Molecular characterization of bot flies, Oestrus spp., (Diptera, Oestridae), from domestic and wild Bovidae hosts

Cited by 20 publications

References 23 publications

Molecular Characterization and Phylogenetic Analyses of <i>Oestrus ovis</i> Larvae Causing Human Naso-pharyngeal Myiasis Based on CO1 Barcode Sequences

Molecular Characterization and Phylogenetic Analyses of <i>Oestrus ovis</i> Larvae Causing Human Naso-pharyngeal Myiasis Based on CO1 Barcode Sequences

Sequence analyzing of Oestrus ovis in sheeps in Al-Diwaniyah province

Immune humoral response of young lambs naturally infested by Oestrus ovis (Diptera: Oestridae)

Contact Info

Product

Resources

About