Molecular Characterization of blaIMP–4-Carrying Enterobacterales in Henan Province of China

Liu, Wentian; Dong, Huiyue; Yan, Tingting; Liu, Xuchun; Cheng, Jing; Liu, Congcong; Zhang, Songxuan; Feng, Xiaohai; Liu, Luxin; Wang, Zhenya; Qin, Shangshang

doi:10.3389/fmicb.2021.626160

Cited by 12 publications

(20 citation statements)

References 33 publications

(32 reference statements)

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“… 7 P. aeruginosa plasmids have multiple incompatibility (Inc) or replicon types, such as IncF, N, HI2, P2, and are associated with the blaIMP gene propagation. 8 , 9 Mutations in P. aeruginosa outer membrane porin D (OprD) reduce carbapenems sensitivity and lead to carbapenem resistance. 10 However, carbapenem resistance due to OprD mutations cannot be horizontally transferred.…”

Section: Introductionmentioning

confidence: 99%

Emergence of Carbapenem-Resistant ST244, ST292, and ST2446 Pseudomonas aeruginosa Clones in Burn Patients in Yunnan Province

Fang¹,

Baloch²,

Zhang³

et al. 2022

IDR

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Introduction The prevalence of carbapenem-resistant Pseudomonas aeruginosa is increasing persistently, particularly in burn ward isolates. Here, we investigate the prevalence of carbapenem-resistant Pseudomonas aeruginosa in a burn ward of a provincial-level hospital at Kunming, Yunnan province, China. Methods A total of 118 P. aeruginosa strains were isolated from 57 hospitalized patients, and their MICs were measured. Carbapenem-resistant isolates were selected for multilocus sequence typing (MLST). Carbapenem-resistance mechanisms were identified by examining carbapenemase genes and OprD protein and Carba-NP testing. Representative isolates were further characterized by de novo sequencing for carbapenemase molecular background. Results Among 118 P. aeruginosa isolates, 54 (54/118,45.8%) were carbapenem-resistant Pseudomonas aeruginosa , and 3 genotypes were found (ST292, ST244, and ST2446). Non-carbapenemase-producing ST292 was the most prevalent ST, followed by ST2446 and ST244. A novel 13-bp oprD deletion was found in the ST292 clone, which formed the truncated outer membrane protein and may cause carbapenem resistance. ST244 and ST2446 harbored blaIMP-45 and blaIMP-87 , respectively. blaIMP-45 is located in a megaplasmid, together with aac(6’)-Ib3, blaOXA-1, catB3, qnrVC6, armA, msr(E), mph(E), aph(3’)-Ia, tetC / tetR, aac(6’)-Ib3, floR, mexC-mexD-oprJ, fosA and lead to extensive drug resistance. ST2446 contains a carbapenem-resistant gene blaIMP-87 on the chromosome and is acquired by a novel gene cassette array ( blaIMP-87-ant(2”)-Ia-blaOXA-10-aac(6’)-Ib3 ) of class 1 integron. Discussion For the first time, ST244, ST292 and ST2446 are reported emerging in burn patients, with distinctive carbapenem-resistance mechanisms, respectively. The obtained results highlight the need to surveillance carbapenem-resistant isolates in burn patients.

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Section: Introductionmentioning

confidence: 99%

Emergence of Carbapenem-Resistant ST244, ST292, and ST2446 Pseudomonas aeruginosa Clones in Burn Patients in Yunnan Province

Fang¹,

Baloch²,

Zhang³

et al. 2022

IDR

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“…In addition to integrons and the conjugative plasmids, the insertion sequence also plays an important role in the transmission of resistant genes. Previous studies about bla IMP−4 -carrying plasmids emphasized the role of the IS26 mobile element, which may play an important role in the dissemination of IMP-4 in different plasmids (Xu et al, 2020;Liu et al, 2021); the corresponding situation also exists in our strains. Timely determination of the resistance mechanism and the transmission mechanism of resistance genes is very important for clinical anti-infective treatment and controlling the wide spread of these multi-drug resistant bacteria.…”

Section: Discussionmentioning

confidence: 68%

“…The strains in both studies all harbored a conjugative bla IMP−4 -carrying plasmid, which accounts for the carbapenem resistance phenotype. In both plasmids, the bla IMP−4 gene was found in class 1 integron, identical to the IMP-4-carrying plasmids before (Xu et al, 2020;Liu et al, 2021). Class 1 integrons are responsible for the transmission of the bla IMP gene; so far, many Class 1 integrons carrying the bla IMP−4 gene have been reported, such as In 809, 823, 1,456, 1,460, and 1,589 (Lee et al, 2017;Matsumura et al, 2017;Wang et al, 2017;Dolejska et al, 2018).…”

Section: Discussionmentioning

confidence: 87%

First Report of blaIMP–4 and blaSRT–2 Coproducing Serratia marcescens Clinical Isolate in China

et al. 2021

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Carbapenem-resistant Enterobacterales (CRE) has become a major therapeutic concern in clinical settings, and carbapenemase genes have been widely reported in various bacteria. In Serratia marcescens, class A group carbapenemases including SME and KPC were mostly identified. However, there are few reports of metallo-β-lactamase-producing S. marcescens. Here, we isolated a carbapenem-resistant S. marcescens (S378) from a patient with asymptomatic urinary tract infection which was then identified as an IMP-4-producing S. marcescens at a tertiary hospital in Sichuan Province in southwest of China. The species were identified using MALDI-TOF MS, and carbapenemase-encoding genes were detected using PCR and DNA sequencing. The results of antimicrobial susceptibility testing by broth microdilution method indicated that the isolate S. marcescens S378 was resistant to meropenem (MIC = 32 μg/ml) and imipenem (MIC = 64 μg/ml) and intermediate to aztreonam (MIC = 8 μg/ml). The complete genomic sequence of S. marcescens was identified using Illumina (Illumina, San Diego, CA, United States) short-read sequencing (150 bp paired-end reads); five resistance genes had been identified, including blaIMP–4, blaSRT–2, aac(6′)-Ic, qnrS1, and tet(41). Conjugation experiments indicated that the blaIMP–4-carrying plasmid pS378P was conjugative. Complete sequence analysis of the plasmid pS378P bearing blaIMP–4 revealed that it was a 48,780-bp IncN-type plasmid with an average GC content of 50% and was nearly identical to pP378-IMP (99% nucleotide identity and query coverage).

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“…The transfer capacity of the blaIMP-4 -harboring plasmid was investigated by conjugation experiments, which were conducted using previously described methods. 25 Rifampin-resistant E. coli EC600 was used as the recipient, and the wzx-IMP strain was used as the donor. Transconjugants were selected on Mueller–Hinton (MH) agar supplemented with sodium rifampin (200 µg/mL) and meropenem (2 µg/mL) and identified by detecting antimicrobial susceptibility and resistance genes using PCR.…”

Section: Methodsmentioning

confidence: 99%

Section: Conjugation Experimentsmentioning

confidence: 99%

IncN1 ST7 Epidemic Plasmid Carrying blaIMP-4 in One ST85-Type Klebsiella oxytoca Clinical Isolate with Porin Deficiency

Sun

Wei

2021

IDR

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Purpose Klebsiella oxytoca is an opportunistic pathogen causing nosocomial infections. This study was designed to characterize the genomic features of a carbapenem-resistant K. oxytoca strain and analyze its molecular characteristics. Materials and Methods The strain wzx-IMP was isolated from the blood of a 2-year-old girl diagnosed with acute myeloid leukemia-M7. Species identification was performed, and the minimal inhibitory concentration of the strain was measured. Multilocus sequence typing was performed to identify the subtypes of K. oxytoca . The transfer capacity of the blaIMP-4-harboring plasmid was investigated by conjugation experiments, and the genome characteristics of the strain were examined using whole-genome sequencing. Results wzx-IMP belongs to the ST85 type and is resistant to imipenem and meropenem, which harbored the blaIMP-4 gene. The blaIMP-4 gene was located in an IS26-associated class 1 integron of pwzx_IMP, which contains conserved IncN1-type backbone regions with a replication gene and its accessory structure for plasmid replication. The blaIMP-4-carrying plasmid in wzx-IMP was successfully transferred to Escherichia coli EC600 by conjugation. Whole-genome sequencing showed that the wzx-IMP isolate included the blaOXY-1-1 gene, accompanied by OmpK36 absence. Conclusion We report an ST85-type carbapenem-resistant K. oxytoca strain, which produces blaIMP-4 located in an IncN1-type plasmid and accompanied by OmpK36 porin deficiency.

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Molecular Characterization of blaIMP–4-Carrying Enterobacterales in Henan Province of China

Cited by 12 publications

References 33 publications

Emergence of Carbapenem-Resistant ST244, ST292, and ST2446 Pseudomonas aeruginosa Clones in Burn Patients in Yunnan Province

Emergence of Carbapenem-Resistant ST244, ST292, and ST2446 Pseudomonas aeruginosa Clones in Burn Patients in Yunnan Province

First Report of blaIMP–4 and blaSRT–2 Coproducing Serratia marcescens Clinical Isolate in China

IncN1 ST7 Epidemic Plasmid Carrying blaIMP-4 in One ST85-Type Klebsiella oxytoca Clinical Isolate with Porin Deficiency

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