Molecular Characterization by Rapds and Micropropagation of Italian Hazelnut Cultivars

Bacchetta, Loretta; Bernardini, C.; Stefano, G. Di; Pelliccia, O.; Cavicchioni, G; Bonito, R. Di

doi:10.17660/actahortic.2005.686.12

Cited by 7 publications

(6 citation statements)

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“…In this case, Na-merthiolate proved more effective than Na-hypochlorite, because it is able to penetrate cells without causing necrosis (data not shown). Moreover, according to our results, isolated buds were the most suitable explants for in vitro establishment of hazelnut dormant cultivars; the percentage of healthy responsive explants was higher in single buds than in uninodal explants (Bacchetta et al, 2005). The same considerations were reported by Messeguer and Mele (1987) for the in vitro establishment of the Spanish cv.…”

Section: Resultssupporting

confidence: 84%

“…Even if, in some regions, this crop is not the major agricultural resource, it nevertheless represents an interesting source of income for the local sustainable production system and a precious food for traditional local use. Italy, the world's second largest producer, boasts several traditional cultivars, which are mainly cultivated in Campania, Latium, Piedmont, and Sicily with a large number of local genotypes (Bacchetta et al, 2005). In the last few years, some of the major cultivars (Tonda Romana from Latium, Tonda di Giffoni from Campania, and Tonda delle Langhe from Piedmont) obtained the European Community quality stamp for their quality and traditional peculiarity.…”

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confidence: 99%

“…Moreover, several media formulations have been proposed for optimizing shoot multiplication and elongation in the main hazelnut cultivars (Andres et al, 2002;Damiano et al, 2005;Messeguer and Mele, 1987;Yu and Reed, 1993). However, when the standardized protocol was applied to local and minor cultivars, the results were contradictory and some difficulties in shoot growth were observed (Bacchetta et al, 2005). recently proposed a novel method for medium formulation based on the concept that nut nutritional reserves are able to guarantee suitable conditions for seedlings as well as for in vitro shoots.…”

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confidence: 99%

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In Vitro Propagation of Traditional Italian Hazelnut Cultivars as a Tool for the Valorization and Conservation of Local Genetic Resources

Bacchetta¹,

Aramini²,

Bernardini³

et al. 2008

horts

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The hazelnut (Corylus avellana) is one of the most important crops in the Mediterranean basin. The availability of efficient and reliable in vitro propagation could valorize the local genetic resources. Different studies have been carried out for the definition of an efficient hazelnut micropropagation protocol. These have usually been performed on the most important cultivars, but the application of the micropropagation protocol to the minor ones has produced contradictory results and the technique sometimes had less success than the traditional one. The aim of this work was to gather knowledge and additional information on the in vitro performance of some minor cultivars in comparison with the most used for micropropagation. A revised procedure for the specific medium formulation is suggested. The sterilization and culture establishment phases are discussed in detail. The role of zeatin and 6-benzylamminopurine (BA) in shoot proliferation in the Italian traditional cultivars is compared to improve this phase. The rooting stage proves to be one of the most crucial steps in achieving a large-scale commercial application of hazelnut micropropagation.

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Section: Resultssupporting

confidence: 84%

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confidence: 99%

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confidence: 99%

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In Vitro Propagation of Traditional Italian Hazelnut Cultivars as a Tool for the Valorization and Conservation of Local Genetic Resources

Bacchetta¹,

Aramini²,

Bernardini³

et al. 2008

horts

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“…In other trials, Bacchetta et al (2005) concluded that zeatin (1 mg/L) was more suitable than BAP (0.5 mg/L) for shoot elongation.…”

Section: Discussionmentioning

confidence: 92%

Effect of cytokinin type and concentration on in vitro shoot proliferation of hazelnut (Corylus avellanaL.)

Thomson¹,

Deering²

2011

New Zealand Journal of Crop and Horticultural Science

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Corylus avellana L. (hazelnut) is traditionally multiplied by layering because it is difficult to propagate vegetatively using shoot cuttings or grafting. Over the past two decades, in vitro micropropagation has become a successful technique for rapidly building large numbers of many new plant cultivars, but success with hazel remains mixed. The limiting factor for hazel has been low rates of in vitro shoot proliferation. Our study of hazel cultivar 'Daviana' examined the response of shoot multiplication to different cytokinin types and concentrations with the aim of maximizing new plant yields for commercial benefit. Four cytokinins (BAP, kinetin, zeatin, IPA) were evaluated at three concentrations. BAP at 5 mg/L (22.2 mM) was effective for initiation and elongation of new shoots when used with 'Knoxfield2' medium formulated for hazel. New shoot initiation was lowest when the medium contained kinetin.Keywords: cytokinin; Corylus avellana; filbert; hazelnut; in vitro; micropropagation IntroductionAs with most tree crops it is necessary to propagate new Corylus avellana L. (hazelnut, hazel, filbert) plants asexually in order to maintain the genotype and its cropping characteristics in the next generation. However, traditional vegetative propagation of this species is still difficult. Hazel plants are often multiplied by layering stems in stool-beds to exploit a natural tendency to sucker (i.e. develop adventitious shoots) but these beds may take many seasons to establish commercial viability. Hazel is also challenging to multiply using traditional systems that utilize shoot cuttings, grafting or budding. More specialized procedures are required for successful adventitious rooting and callus formation (e.g. hot-callus grafting) but these too are relatively slow processes that prevent rapid build-up of new, favoured cultivars as nursery stock. Expansion of the hazelnut industry in southeastern

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“…According to our knowledge, the recovery of various Sicilian hazelnut genotypes, and also the success of multiplication and sanitation, in a single step after sowing, are reported for the first time. Yet, the rooting stage is the most sensitive step ( Bacchetta et al, 2004 ). This phase needs to be further investigated to achieve synthetic seed conversion in one single step.…”

Section: Discussionmentioning

confidence: 99%

Is It Possible to Produce Certified Hazelnut Plant Material in Sicily? Identification and Recovery of Nebrodi Genetic Resources, in vitro Establishment, and Innovative Sanitation Technique From Apple Mosaic Virus

et al. 2021

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Eight Sicilian cultivars of hazelnut (Corylus avellana L.), namely-Curcia, Nociara Collica, Panottara Collica, Panottara Galati Grande, Parrinara, Panottara Baratta Piccola, Enzo, and Rossa Galvagno, registered into the Italian Cultivar Register of fruit tree species in 2017 were selected from Nebrodi area and established in vitro. The aim of the work was to carry out the sanitation of the cultivars and get virus-free plants from the most important viral pathogen threat, the apple mosaic virus. Virus-free plant material is essential for the production of certified plants from Sicilian hazelnut cultivars, complying the CE (cat. CAC) quality and the technical standards established in 2017 for voluntary certification by the Italian Ministry of Agricultural, Food and Forestry Policies (MIPAAF). In this study, we investigated the possibility of establishing in vitro true-to-type and virus-free hazelnut plantlets via the encapsulation technology of apexes. The in vitro shoot proliferation rates were assessed for the different cultivars, sampling periods, temperature treatments, and type of explant used for culture initiation. Viability, regrowth, and conversion rates of both conventional meristem tip culture (MTC) and not conventional (MTC combined with the encapsulation technology) sanitation techniques were evaluated.

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Molecular Characterization by Rapds and Micropropagation of Italian Hazelnut Cultivars

Cited by 7 publications

References 0 publications

In Vitro Propagation of Traditional Italian Hazelnut Cultivars as a Tool for the Valorization and Conservation of Local Genetic Resources

In Vitro Propagation of Traditional Italian Hazelnut Cultivars as a Tool for the Valorization and Conservation of Local Genetic Resources

Effect of cytokinin type and concentration on in vitro shoot proliferation of hazelnut (Corylus avellanaL.)

Is It Possible to Produce Certified Hazelnut Plant Material in Sicily? Identification and Recovery of Nebrodi Genetic Resources, in vitro Establishment, and Innovative Sanitation Technique From Apple Mosaic Virus

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