Molecular characterization and phylogenetic position of Rotylenchus pini Mamiya, 1968 (Nematoda: Hoplolaimidae) from Korea, with remarks on its morphology and morphometrics

A Revision of the Phylogeny of Helicotylenchus Steiner, 1945 (Tylenchida: Hoplolaimidae) as Inferred from Ribosomal and Mitochondrial DNA

“…Polymerase chain reaction (PCR) was performed with a PCR cycler (T100, Bio-Rad, Hercules, CA, USA), the PCR program with specific primers being set as follows: initial denaturation at 95°C for 5 min, 35 cycles at 95°C for 30 s, followed by an annealing step at 58°C for 30 s (S-ITS1/Vrain2R), 54°C for 40 s (COIHF/COIPR), 56°C for 40 s (COIF5/COIR9); 72°C for 60 s (S-ITS1/Vrain2R), 72°C for 80 s (COIHF/COIPR and COIF5/COIR9), and finally one cycle at 72°C for 5 min (COIHF/COIPR) and 72°C for 10 min (S-ITS1/Vrain2R and COIF5/COIR9). The cycle for D2A/D3B and JB3/JB5 primer sets was as described by Mwamula et al (2023a) while the thermal profiles for TW81/AB28, 988F/1912R and 1813F/2646R primer sets were set as detailed by Mwamula et al (2023b) . The PCR products were purified using a PCR purification kit (Qiagen, Hilden, Germany) and quantified using a quickdrop spectrophotometer (Molecular Devices, San Jose, CA, USA).…”

“…described by Mwamula et al (2023a) while the thermal profiles for TW81/ AB28, 988F/1912R and 1813F/2646R primer sets were set as detailed by Mwamula et al (2023b). The PCR products were purified using a PCR purification kit (Qiagen, Hilden, Germany) and quantified using a quickdrop spectrophotometer (Molecular Devices, San Jose, CA, USA).…”