Molecular characterization and phylogenetic analysis of small ruminant lentiviruses isolated from Canadian sheep and goats

L’Homme, Yvan; Ouardani, Mourad; Lévesque, Valérie; Bertoni, Giuseppe; Simard, C; Pisoni, G.

doi:10.1186/1743-422x-8-271

Cited by 27 publications

(21 citation statements)

References 20 publications

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“…There were 17 amino acid deletions in the Philippine CAEV isolate that could indicate that it is a new strain of the virus. In accordance with the study of L'Homme et al [31], the present study also showed that CAEV sequences isolated from goats contained a double glycine ''GG'' motif ( Fig. 1) that is characteristic of the CAEV-Cork lineage, and they were most similar to prototypical CAEV strains.…”

Section: Resultssupporting

confidence: 93%

“…The partial and complete coding sequences of the gag gene of CAEV from area 1 (Northern Philippines) and area 3 (Southern Philippines) ( Table 2) were successfully amplified using primers designed to detect proviral DNA of goats infected with caprine lentiviruses in the province of Quebec, Canada [31], and newly designed primers based on the CAEV Shanxi strain ( Table 1).…”

Section: Resultsmentioning

confidence: 99%

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Molecular characterization of the gag gene of caprine arthritis encephalitis virus from goats in the Philippines

et al. 2015

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Caprine arthritis encephalitis virus (CAEV) causes caprine arthritis encephalitis syndrome, which is an emerging disease of goats in the Philippines. DNA sequence analysis showed homology of 86-93 % between Philippine CAEV and available CAEV sequences in GenBank. CAEV was detected using nested polymerase chain reaction (PCR), and new sets of primers were designed in order to amplify the gag gene, which is a highly conserved region of the viral genome. In addition, the Philippine CAEV isolate clustered in group B with the prototype caprine lentivirus. Based on amino acid sequence alignments, it is possible that the Philippine CAEV isolate is a new strain of CAEV, but it is also possible that it was already present in the country even before the start of goat importation. Molecular characterization of the CAEV gag gene is important for the development of a detection kit specific for the local strain of CAEV and the establishment of small ruminant lentivirus eradication programs in the Philippines. This study is the first report to describe the molecular characteristics of CAEV circulating in the Philippines.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Molecular characterization of the gag gene of caprine arthritis encephalitis virus from goats in the Philippines

et al. 2015

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“…The members of this SRLV group in the Retroviridae family, maedi-visna virus (MVV) and caprine arthritis-encephalitis virus (CAEV), infect sheep and goats (L'Homme et al 2011). Small ruminant lentiviruses cause persistent infections which can induce systemic diseases affecting joints, mammary glands and respiratory and central nervous systems after long incubation periods (Narayan et al 1983).…”

mentioning

confidence: 99%

“…The gag and pol genes are relatively well conserved among SRLVs, which makes them ideal targets for Polymerase Chain Reaction (PCR) primer design (Pepin et al 1998;Grego et al 2007). The MVV prototypical strains such as strain K1514, EV-1, and SA-OMVV from sheep and the CAEV strain Cork-CAEV isolated from goat (L'Homme et al 2011) were originally viewed as distinct viral species restricted to respective host animal species. As more SRLV sequences had become available for phylogenetic analyses, it became evident that SRLVs can cross the species barrier and can be transmitted from sheep to goats under favourable conditions (Shah et al 2004b;Pisoni et al 2005).…”

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confidence: 99%

Genetic characterisation of small ruminant lentiviruses in sheep and goats from the Czech Republic

et al. 2018

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The aim of this study was to determine the prevalence of small ruminant lentivirus (SRLV) infections on sheep and goat farms which are exempt from state monitoring and carry molecular characterisation of strains circulating amongst these farms without SRLV eradication. A total number of 3,410 blood samples of sheep and goats from 21 herds were collected for the purpose of the project. The detected serological prevalence of maedi visna in sheep was 19.9% (556/2801) and the seroprevalence of caprine arthritis and encephalitis in goats was 14.1% (86/609). All positive animals were tested by the nested polymerase chain reaction (nPCR) method for the presence of provirus in the buffy-coats from EDTA-blood samples. Phylogenetic analysis of 93 SRLV strains identified the genotype in 77 sequences, where 60 of them were genotype A and 17 belonged to genotype B. Whereas all of the genotype B sequences were classified in subtype B2, the genotype A group of isolates showed higher variability and were related to subgenotypes A2 and A3. This study represents the first report of genetic characterisation of SRLV strains circulating in the territory of the Czech Republic. Maedi visna, caprine arthritis, nested PCR, ELISA

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“…Furthermore, the three goats were finally confirmed for CAEV appearance in their blood by PCR technique according to a method described previously (17). The primers were designed corresponding to the most highly conserved sequences in the gag regions from available small ruminant lentivirus genomes in public databases to produce a 1327-bp amplicon.…”

Section: Goat Samplesmentioning

confidence: 99%

Cocultivation of caprine arthritis encephalitis virus-infected macrophages with primary goat synovial cells

Panneum¹,

Chumsing²,

Rukkwamsuk³

2017

Turk J Vet Anim Sci

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Primary goat synovial cells were developed to be cocultivated with caprine arthritis encephalitis virus (CAEV)-infected monocytes. Two CAEV-seropositive goats and one CAEV-seronegative goat were confirmed for viral particles in the blood using the PCR method. Thereafter, heparinized blood samples from the three goats were collected and isolated for monocytes, which were further cultivated to differentiate into macrophages. Cultivated macrophages of 7-10 days old were cocultivated with the primary goat synovial cells for 7 days. Microscopic examination was performed every 2 days for multinucleated syncytial formation, and immunofluorescence assay was applied using monoclonal antibody against CAEV on day 7 to detect viral particles. Results showed that the primary goat synovial cells cultivated with CAEV-infected macrophages from the two seropositive goats had multinucleated syncytial formation with positive immunofluorescence, while the cocultured macrophages from the seronegative goats showed normal appearance with negative immunofluorescence. The multinucleated syncytial cells were prepared for transmission electron microscope examination and the results indicated that CAEV particles were clearly identified. In conclusion, our developed primary goat synovial cells and the cultivating system were proven to be an appropriate isolation assay for CAEV, which was the first report of cultivation and isolation of CAEV in goats in Thailand.

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Molecular characterization and phylogenetic analysis of small ruminant lentiviruses isolated from Canadian sheep and goats

Cited by 27 publications

References 20 publications

Molecular characterization of the gag gene of caprine arthritis encephalitis virus from goats in the Philippines

Molecular characterization of the gag gene of caprine arthritis encephalitis virus from goats in the Philippines

Genetic characterisation of small ruminant lentiviruses in sheep and goats from the Czech Republic

Cocultivation of caprine arthritis encephalitis virus-infected macrophages with primary goat synovial cells

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